Immunohistochemical localization of synaptophysin (p38) in the pineal gland of the Mongolian gerbil (Meriones unguiculatus)

Redecker, P.; Grube, D.; Jahn, Reinhard

doi:10.1007/bf02433790

Cited by 26 publications

(36 citation statements)

References 36 publications

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“…It has been shown that SLMVs are present in large numbers within pinealocytes of the mammalian pineal gland and that pinealocytes contain several proteins which function in the regulation of synaptic vesicle trafficking in the nerve terminal (Vollrath and Schröder, 1987;Redecker et al, 1990;Huang et al, 1992;Redecker, 1993;Redecker and Bargsten, 1993;Redecker, 1995Redecker, , 1996Redecker et al, 1996). Moreover, increasing evidence supports the contention that SLMVs of pinealocytes store neuroactive amino acids as signal molecules (Redecker and Veh, 1994;Moriyama and Yamamoto, 1995).…”

Section: Introductionmentioning

confidence: 91%

Expression of synaptic membrane proteins in gerbil pinealocytes in primary culture

Redecker¹,

Pabst²,

Gebert³

et al. 1997

J. Neurosci. Res.

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Section: Introductionmentioning

confidence: 91%

Expression of synaptic membrane proteins in gerbil pinealocytes in primary culture

Redecker¹,

Pabst²,

Gebert³

et al. 1997

J. Neurosci. Res.

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“…Polyclonal rabbit antibodies against NSE, PGP 9.5 and S-100 were purchased from Dako (Hamburg, Germany), UltraClone (Cambridge, UK) or Sigma (Deisenhofen, Germany) respectively (for references, see Redecker et al, 1990;Lauweryns & Seldeslagh, 1993;Romeo et al, 1993). A monoclonal antibody against vimentin (V 9) was supplied by Boehringer (Mannheim, Germany; see Osborn et al, 1984;Redecker et al, 1990).…”

Section: Antibodiesmentioning

confidence: 99%

“…For the immunocytochemical localization of S-Ag', we used a rabbit antiserum (NEI 04111083) that was obtained after immunization with highly purified bovine S-Ag (Korf et al, 1985(Korf et al, , 1986. Polyclonal rabbit antibodies against NSE, PGP 9.5 and S-100 were purchased from Dako (Hamburg, Germany), UltraClone (Cambridge, UK) or Sigma (Deisenhofen, Germany) respectively (for references, see Redecker et al, 1990;Lauweryns & Seldeslagh, 1993;Romeo et al, 1993). A monoclonal antibody against vimentin (V 9) was supplied by Boehringer (Mannheim, Germany; see Osborn et al, 1984;Redecker et al, 1990).…”

Section: Antibodiesmentioning

confidence: 99%

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Differential immunocytochemical localization of calretinin in the pineal gland of three mammalian species

1996

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Calcium plays an important role for signal transduction in the mammalian pineal organ. The regulation of the intracellular concentration of free calcium probably involves calcium-binding proteins of the calmodulin superfamily. In the present study, we have investigated the expression of calretinin, one member of this superfamily, in the pineal organ of hamsters, gerbils and guinea-pigs by means of immunochemical and immunocytochemical analyses with a calretinin-specific antiserum. In immunoblots this antibody recognized a single protein band of approximately 29 kDa in the brain and pineal organ of all three mammalian species. Immunocytochemical investigations of serial semithin sections of plastic-embedded pineals revealed the constant occurrence of variable numbers of calretinin-positive cells throughout all glands. In order to identify the immunopositive cells precisely, adjacent sections were exposed to antibodies against various marker proteins of pineal cell types, i.e., synaptophysin, neuron-specific enolase, protein gene product 9.5, S-antigen, vimentin and S-100. By this approach, calretinin could be localized to vimentin-positive cells in the gerbil which are generally considered as interstitial glial cells. Likewise, calretinin-positive cells in the guinea-pig probably correspond to interstitial cells, taking into account their morphology and the lack of calretinin immunoreactivity in pinealocytes. The unusual expression of calretinin in astrocyte-like cells further supports the notion that pineal glial cells are endowed with peculiar properties. In contrast to gerbil and guinea-pig, a subpopulation of pinealocytes displayed calretinin immunoreactivity in the hamster. This finding adds to the hypothesis that in pinealocytes of some species calretinin plays a role in calcium-mediated signal transduction which eventually is linked to melatonin synthesis. Our results demonstrate that calretinin is a regular constituent of pineal glands in three mammalian species, but that its cellular localisation shows interspecific variation. This variation suggests that the protein is involved in diverse calcium-mediated functions in the mammalian pineal gland.

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“…The serial sections were alternatively immunostained for pitu itary hormones and synaptic vesicle proteins. Immunostaining was performed as described previously [3,24,25]. Briefly, semithin sec tions were immunostained by the avidin-biotin-peroxidase complex (ABC) technique [26].…”

Section: Immunocytochemical Protocolmentioning

confidence: 99%

Differential Distribution of Synaptotagmin I and rab3 in the Anterior Pituitary of Four Mammalian Species

Redecker

Cetin²,

Grube³

1995

Neuroendocrinology

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In the anterior pituitary of rat, gerbil, hamster and guinea pig, the presence and cellular distribution of the synaptic vesicle-associated proteins synaptotagmin I and rab3 were analyzed by immunoblotting and by immunocytochemical staining of serial semithin sections. Our results show that rab3 proteins are ubiquitously expressed in all endocrine cell types of both the anterior and intermediate lobe. In many cells, rab3 immunoreactivity was concentrated beneath the plasmalemma. This intracellular distribution coincided with the distribution of secretory granules, suggesting a possible association of rab3 proteins with the latter organelles. The staining patterns observed using two monoclonal rab3 antibodies with different isoform specificities are compatible with the recent suggestion that rab3B is the dominant rab3 isoform in anterior pituitary cells. However, we could demonstrate that also rab3A is present in endocrine adenohypophyseal cells, albeit at low levels. In contrast to rab3, synaptotagmin I immunoreactivity was only detected in a limited number of adenohypophyseal endocrine cells. Whereas the monoclonal synaptotagmin I antibody consistently failed to immunostain lactotrophs and endocrine cells of the intermediate lobe, other endocrine cell types displayed variable immunoreactivities towards this antibody. Although a low level of synaptotagmin I expression in the immunonegative cells cannot be excluded, the above observation may reflect a differential distribution of synaptotagmin isoforms in endocrine organs, as it has been described for the nervous system. Our study has established that endocrine cells of the anterior pituitary are endowed with proteins of the rab3 and synaptotagmin families which are generally thought to play important roles in the regulation of the trafficking and/ or exocytosis of secretory organelles and, hence, probably fulfil similar functions in adenohypophyseal cells.

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Immunohistochemical localization of synaptophysin (p38) in the pineal gland of the Mongolian gerbil (Meriones unguiculatus)

Cited by 26 publications

References 36 publications

Expression of synaptic membrane proteins in gerbil pinealocytes in primary culture

Expression of synaptic membrane proteins in gerbil pinealocytes in primary culture

Differential immunocytochemical localization of calretinin in the pineal gland of three mammalian species

Differential Distribution of Synaptotagmin I and rab3 in the Anterior Pituitary of Four Mammalian Species

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