Immunohistochemical Localization of Taurine in the Rat Ovary, Oviduct, and Uterus

Lobo, M.V. Toledo; Alonso, F.J. Martínez; Latorre, Amparo; Rı́o, Rafael Martı́n del

doi:10.1177/002215540104900907

Cited by 18 publications

(13 citation statements)

References 42 publications

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“…In the uterine fluid, concentration of Tau is fairly high in contrast to the bovine blood plasma or oviduct fluid (Hugentobler et al 2007). Lowered Tau amounts in rat uterine epithelial cells were confirmed during the estrous phase as well as during pregnancy and epithelial cells contain large amounts of Tau at diestrus (Lobo et al 2001). We demonstrated an increase in the expression of transcripts for AA transporter for b-AA (which include Tau and bAla) SLC6A6 (TAUT) over time, which could account for elevated Tau in the uterine lumen.…”

Section: Intrauterine Amino Acids During Early Pregnancymentioning

confidence: 86%

Increase of essential amino acids in the bovine uterine lumen during preimplantation development

Groebner

Rubio‐Aliaga²,

Schülke³

et al. 2011

Reproduction

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Amino acids (AAs) are crucial for the developing conceptus prior to implantation. To provide insights into the requirements of the bovine embryo, we determined the AA composition of the uterine fluid. At days 12, 15, and 18 post-estrus, the uteri of synchronized pregnant and non-pregnant Simmental heifers were flushed for the analysis of 41 AAs and their derivatives by liquid chromatography-tandem mass spectrometry. The ipsilateral endometrium was sampled for quantitative PCR. In addition to a pregnancy-dependent increase of the essential AAs (P!0.01), we detected elevated concentrations for most non-essential proteinogenic AAs. Histidine (His) and the expression of the His/peptide transporter solute carrier 15A3 (SLC15A3) were significantly increased at day 18 of pregnancy in vivo. In addition, SLC15A3 was predominantly stimulated by trophoblast-derived interferon-t in stroma cells of an in vitro co-culture model of endometrial cells. Our results show an increased concentration of AAs most likely to optimally provide the elongating pre-attachment conceptus with nutrients.

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Section: Intrauterine Amino Acids During Early Pregnancymentioning

confidence: 86%

Increase of essential amino acids in the bovine uterine lumen during preimplantation development

Groebner

Rubio‐Aliaga²,

Schülke³

et al. 2011

Reproduction

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“…Oyster gametes most likely accumulate osmolytes, such as taurine, because they will be released into the sea water. In addition, in mammals, the reproductive tissues contain high amounts of taurine, which is thought to play multiple physiological roles in these tissues 33,34 …”

Section: Discussionmentioning

confidence: 99%

Taurine transporter from the giant Pacific oyster Crassostrea gigas: function and expression in response to hyper- and hypo-osmotic stress

et al. 2007

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Taurine is the primary osmolyte in marine molluscs, whose cellular osmo-conforming process is vital for environmental adaptation because of a lack of osmotic homeostasis. Here, cDNA cloning and expression, and functional analyses of taurine transporter (TAUT) from the giant Pacific oyster are reported on. The deduced amino-acid sequence of oyster TAUT (oyTAUT) showed 47-51% identity to those of vertebrate TAUT, whereas identity among the vertebrates is 78-95%. Functional analysis of oyTAUT expressed in Xenopus oocytes revealed that oyTAUT has a lower affinity and specificity for taurine and a requirement for higher NaCl concentration, compared with vertebrate TAUT. Taken together with similar functional properties of TAUT from mussel, indicated by our previous study, it is possible that these functional features reflect the internal environment of the molluscs (i.e. higher taurine and NaCl concentrations). Oyster taurine transporter mRNA expression was induced by not only hyper-osmotic stress, similar to other TAUT, but also hypo-osmotic stress. It is speculated that the expression in response to hypo-osmotic stress was induced by a substantial decrease in tissue taurine content following the decrease in the internal osmolality.

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“…Slc6a6, also known as the taurine transporter, is highly expressed in ciliated cells (13,14). As its name implies, Slc6a6 pumps taurine into cells and is critical for the development of vision and hearing and in skeletal muscles and the central nervous system (22)(23)(24).…”

Section: Depletion Of Mir-183/96 Leads To Abolished Photopic Erg Respmentioning

confidence: 99%

“…Slc6a6, a taurine transporter, is highly expressed in ciliated cells (13,14). In diseased retinas, Slc6a6 acts as a neuronal protectant by regulating several "essential genes" of the photoreceptors via the retinal long noncoding RNA Tug1 to protect the photoreceptors from degeneration (15).…”

mentioning

confidence: 99%

miR-183/96 plays a pivotal regulatory role in mouse photoreceptor maturation and maintenance

Xiang

Chen

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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MicroRNAs (miRNAs) are known to be essential for retinal maturation and functionality; however, the role of the most abundant miRNAs, the miR-183/96/182 cluster (miR-183 cluster), in photoreceptor cells remains unclear. Here we demonstrate that ablation of two components of the miR-183 cluster, miR-183 and miR-96, significantly affects photoreceptor maturation and maintenance in mice. Morphologically, early-onset dislocated cone nuclei, shortened outer segments and thinned outer nuclear layers are observed in the miR-183/96 double-knockout (DKO) mice. Abnormal photoreceptor responses, including abolished photopic electroretinography (ERG) responses and compromised scotopic ERG responses, reflect the functional changes in the degenerated retina. We further identify Slc6a6 as the cotarget of miR-183 and miR-96. The expression level of Slc6a6 is significantly higher in the DKO mice than in the wild-type mice. In contrast, Slc6a6 is down-regulated by adenoassociated virus-mediated overexpression of either miR-183 or miR-96 in wild-type mice. Remarkably, both silencing and overexpression of Slc6a6 in the retina are detrimental to the electrophysiological activity of the photoreceptors in response to dim light stimuli. We demonstrate that miR-183/96-mediated fine-tuning of Slc6a6 expression is indispensable for photoreceptor maturation and maintenance, thereby providing insight into the epigenetic regulation of photoreceptors in mice.miR-183/96/182 cluster | regulation | photoreceptor | taurine transporter | degeneration M icroRNAs (miRNAs) are known to act as important epigenetic coordinators during posttranscriptional processing via the regulation of hundreds of target genes with great temporal and spatial precision (1-3). Wholesale and individual disruption of miRNAs has been shown to result in various retinal defects and other sensorial diseases (4-6). The retinal photoreceptor is a type of ciliated neuron in which the miR-183 cluster represents the most highly expressed miRNAs. Although different mouse models have been generated to determine the roles of the miR-183 cluster, many previous studies have used nonspecific or incomplete miRNA depletion to examine the effects of eliminating specific miRNAs on photoreceptor degeneration (5, 6). Little is known about the impact of a "null" miR-183/96/182 model in vivo.A recent study of miR-182 and miR-183 identified these two miRNAs as essential for the maturation and function of cone photoreceptors (7). In addition, their expression is necessary and sufficient for the formation of cone outer segments (OSs) (7). Another study likewise showed that inactivation of the miR-183 cluster by gene trapping resulted in abnormal electroretinography (ERG) responses, progressive synaptic defects, and progressive retinal degeneration (4). Other investigators have reported that impairment of miR-96 resulted in hair cell death in the inner ear, as well as progressive hearing loss in zebrafish, mice, and humans (8-10). Notwithstanding these findings, however, our understanding of the re...

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Immunohistochemical Localization of Taurine in the Rat Ovary, Oviduct, and Uterus

Cited by 18 publications

References 42 publications

Increase of essential amino acids in the bovine uterine lumen during preimplantation development

Increase of essential amino acids in the bovine uterine lumen during preimplantation development

Taurine transporter from the giant Pacific oyster Crassostrea gigas: function and expression in response to hyper- and hypo-osmotic stress

miR-183/96 plays a pivotal regulatory role in mouse photoreceptor maturation and maintenance

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