Immunohistochemical Localizations of Class II Antigens and Nerve Fibers in Human Carious Teeth: HLA-DR Immunoreactivity in Schwann Cells.

Yoshiba, Nagako; Yoshiba, Kunihiko; Iwaku, Masaaki; Ozawa, Hiroki

doi:10.1679/aohc.61.343

Cited by 13 publications

(9 citation statements)

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“…These results suggest that the nerve fibers may be involved in reparative dentinogenesis, after cavity preparation has been completed. Also of note is the intimate association of MHC class II‐expressing cells and the PGP 9.5‐positive nerve fibers in human carious teeth, suggesting their functional interactions in dental pulp (27, 31). A similar situation would occur during tissue repair after cavity preparation.…”

Section: Discussionmentioning

confidence: 99%

“…Based on the protocol for double‐immunofluorescence staining described in a previous study (31), free‐floating sections were incubated in PBS containing OX6 monoclonal antibodies (Serotec) at a dilution of 1 : 5000 for 24 h at 4°C. These antibodies were capable of recognizing Ia antigens.…”

Section: Methodsmentioning

confidence: 99%

“…Using confocal laser scanning microscopy and light microscopy, a number of changes have been reported in the distribution patterns and overall responses of class II-expressing cells in experimentally induced pulpitis (29), subsequent to cavity preparation (30), and beneath caries (27,31). More recently, the intimate association of MHC class II-expressing cells and nerve fibers has been demonstrated in carious human teeth, suggesting their functional interactions within dental pulp (31). The present study seeks to investigate pulpal reactions to cavity preparations in rat molars using Er:YAG lasers.…”

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confidence: 99%

“…Class II major histocompatibility complex (MHC) antigen‐expressing cells are thought to act as antigen‐expressing cells that can absorb and process complex antigens and present them to lymphocytes – a function essential to the initiation of immune responses (28). Using confocal laser scanning microscopy and light microscopy, a number of changes have been reported in the distribution patterns and overall responses of class II‐expressing cells in experimentally induced pulpitis (29), subsequent to cavity preparation (30), and beneath caries (27, 31). More recently, the intimate association of MHC class II‐expressing cells and nerve fibers has been demonstrated in carious human teeth, suggesting their functional interactions within dental pulp (31).…”

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Immunohistochemical study on pulpal response in rat molars after cavity preparation by Er:YAG laser

Tanabe

Yoshiba

et al. 2002

European J Oral Sciences

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While Er:YAG laser systems are in extensive use for caries removal and cavity preparation, the effects of such treatment on pulp tissue remain unclear. This study evaluates these systems using immunohistochemical methods and compares the results with information gained from treatment using conventional burs. Cervical cavities were prepared in the upper first molars of rats, using either an Er:YAG laser or a conventional tungsten-carbide bur. At intervals of 5 min, 6 h, 12 h, 1 d, 3 d and 7 d after cavity preparation, the teeth were processed for immunohistochemical analyses of tissue non-specific alkaline phosphatase, OX6-positive major histocompatibility complex class II antigen-expressing cells and PGP 9.5-immunoreactive nerve fibers. DNA fragmentation was detected by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method. Tissue non-specific alkaline phosphatase was observed mainly in the subodontoblastic layer under the cavity lesion, from 5 min, in both groups. The immunoreactivity was more pronounced in the laser group, but by 7 d no significant differences were recognizable. At 12 h, TUNEL-positive cells were detected around the odontoblastic layer in both groups. From 3 d to 7 d, a limited number of positive cells were still visible in the group that underwent standard treatment. Clear similarities in the distribution patterns of OX6-immunopositive cells and PGP 9.5-immunoreactive nerve fibers were also noted. From 12 h to 1 d, OX6-positive cells accumulated along the pulp-dentin border, extending their processes into the dentinal tubules. Numerous bead-like PGP 9.5-immunoreactive nerve fibers were observed under the odontoblastic layer at 7 d. These results demonstrated that there was no appreciable difference in the manner in which pulp tissue responded to treatment with either Er:YAG laser or a conventional drill. This would seem to indicate the usefulness of the Er:YAG laser system in the removal of caries and cavity preparation.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Immunohistochemical study on pulpal response in rat molars after cavity preparation by Er:YAG laser

Tanabe

Yoshiba

et al. 2002

European J Oral Sciences

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“…Considerable research has focused on mechanisms of immune surveillance during the development of pulpal infections and apical periodontitis (Jontell et al, 1998;Yoshiba et al, 1998;Yoshiba et al, 2003). Toll-4 (TLR4) and CD14 function as pattern recognition receptors for innate immune detection of tissue levels of bacterial-derived factors such as lipopolysacharide (Fitzgerald et al, 2004;Miller et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Trigeminal Nociceptors Express TLR-4 and CD14: a Mechanism for Pain due to Infection

2006

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Although certain bacterial species appear to be risk factors for pain due to odontogenic infections, comparatively little is known about the potential mechanisms mediating this effect. In this study, we tested the hypothesis that trigeminal nociceptive neurons express the TLR4 or CD14 receptors, thus enabling sensory neurons to detect and respond to tissue levels of bacterial substances such as lipopolysaccharide (LPS). Immunohistochemical analyses of human and rat trigeminal neurons demonstrated that a capsaicin-sensitive subclass of nociceptors (defined by expression of TRPV1, a capsaicin receptor) expresses both TLR4 and CD14. Moreover, human dental pulp collected from patients with caries lesions demonstrated co-localization of TLR4 and CD14, with markers of peripheral sensory neurons. Collectively, these studies indicate that the capsaicin-sensitive subclass of trigeminal nociceptors expresses TLR4 and CD14. These results indicate that pain due to bacterial infections may result, in part, from direct activation of nociceptors by bacterial products such as LPS.

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Etiology and Pathogenesis of Pulpitis and Apical Periodontitis

Fouad¹,

Khan²

2019

Essential Endodontology

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Immunohistochemical Localizations of Class II Antigens and Nerve Fibers in Human Carious Teeth: HLA-DR Immunoreactivity in Schwann Cells.

Cited by 13 publications

References 37 publications

Immunohistochemical study on pulpal response in rat molars after cavity preparation by Er:YAG laser

Immunohistochemical study on pulpal response in rat molars after cavity preparation by Er:YAG laser

Trigeminal Nociceptors Express TLR-4 and CD14: a Mechanism for Pain due to Infection

Etiology and Pathogenesis of Pulpitis and Apical Periodontitis

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