Immunohistochemical mapping of calcium-binding protein immunoreactivity in the rat central nervous system

Garcia-Segura, Luis Miguel; Baetens, D.; Roth, Jürgen; Norman, Anthony W.; Orci, Lorenzo A.

doi:10.1016/0006-8993(84)90512-2

Cited by 271 publications

(137 citation statements)

References 23 publications

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“…Lists of CaBPpositive structures, published for birds (Jande et al, 198 1;Roth et al,' 1981) and mammals (Jande et al, 1981;Feldman and Christakos, 1983;Garcia-Segura et al, 1984) attest to its widespread distribution. CaBP immunoreactivity in the avian auditory system has already been reported in that NM, NL, VLV, and inferior colliculus of the chicken are listed as containing immunoreactive fibers or cell bodies (Jande et al, 1981;Roth et al, 198 1).…”

Section: Discussionmentioning

confidence: 99%

“…All neurons of the mammalian ventral cochlear nucleus, some of which phase-lock to acoustic stimuli (Lavine, 197 1;Goldberg and Brownell, 1973;Rose et al, 1974), have been reported to contain CaBP (Jande et al, 198 1;Garcia-Segura et al, 1984) in the rat. The medial superior olive, which also contains phase-locking neurons (Moushegian et al, 1967;Goldberg and Brown, 1969) and is thought to be the mammalian homolog of the avian NL (Ramon y Cajal, 1908), projects to a region in the ventrolateral portion of the ICC (Van Noort, 1969;Elverland, 1978;Henkel and Spangler, 1983).…”

Section: Discussionmentioning

confidence: 99%

“…The medial superior olive, which also contains phase-locking neurons (Moushegian et al, 1967;Goldberg and Brown, 1969) and is thought to be the mammalian homolog of the avian NL (Ramon y Cajal, 1908), projects to a region in the ventrolateral portion of the ICC (Van Noort, 1969;Elverland, 1978;Henkel and Spangler, 1983). CaBP-immunoreactive fibers, which have been reported in the mammalian inferior colliculus (Garcia-Segura et al, 1984), may be restricted to the ventrolateral sector. Finally, it would be of interest to compare the distribution of CaBP immunoreactivity with the distribution of neurons of the mammalian inferior colliculus having various binaural properties (Roth et al, 1978;Semple and Aitkin, 1979;Wenstrup et al, 1986).…”

Section: Discussionmentioning

confidence: 99%

“…It would also represent a counterexample to the association of CaBP with phase-locking ability. The study of Garcia-Segura et al (1984), however, must be interpreted with caution. They define CaBP-negative somata as those that are counterstained with Evan's blue, but give no reason why CaBP immunoreactivity, as visualized by their peroxidase method, should exclude the counterstain.…”

Section: Discussionmentioning

confidence: 99%

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Calcium binding protein-like immunoreactivity labels the terminal field of nucleus laminaris of the barn owl

Takahashi¹,

Carr²,

Brecha³

et al. 1987

J. Neurosci.

101

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Nucleus laminaris (NL) is the site at which the timing of sounds arriving in the 2 ears is compared in the auditory system of the barn owl. Earlier studies have reported vitamin D-dependent calcium binding protein (CaBP)-like immunoreactivity in the somata of NL. We report here that CaBP-like immunoreactivity stains the terminal field of NL. The specific CaBP immunoreactivity is localized to a dense plexus of fibers that have bouton-like swellings, usually around unstained somata. This type of immunoreactivity is found in a restricted portion of the central nucleus of the inferior colliculus (ICc), in the anterior division of the ventral lateral lemniscal complex (VLVA), and in the superior olivary nucleus (SO), all of which have been shown by anterograde transport of 3H-proline to be innervated by NL. The immunoreactivity is absent from the posterior division of ventral lateral lemniscal complex and from the region that surrounds the portion of ICc innervated by NL. A restricted lesion in NL results in a localized deficit in immunoreactivity in those regions of ICc and VLVA that are known to be innervated by the lesioned area of NL. In adjacent sections processed by the Fink-Heimer method, degenerating axons are present in the region of the deficit in immunoreactivity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Calcium binding protein-like immunoreactivity labels the terminal field of nucleus laminaris of the barn owl

Takahashi¹,

Carr²,

Brecha³

et al. 1987

J. Neurosci.

101

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“…We show that calbindin is localized to the somata and to nerve terminals of motor neurons in N.IX-X, a result that contrasts with the apparent absence of calbindin expression in motor neurons in other animal models (Jande et al, 1981;García-Segura et al, 1984;Séquier et al, 1991). Axotomy decreases and androgen increases calbindin immunoreactivity in N.IX-X; as a result, androgens mitigate axotomy-induced decreases in calbindin expression in motor neurons.…”

mentioning

confidence: 57%

Androgen Mitigates Axotomy-Induced Decreases in Calbindin Expression in Motor Neurons

Pérez

Kelley

1997

J. Neurosci.

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Androgens can rescue axotomized motor neurons from cell death. Here we examine a possible mechanism for this trophic action in juvenile Xenopus laevis: regulation of a calciumbinding protein, calbindin, after axotomy. Western analysis revealed that a monoclonal antibody to calbindin D specifically recognizes a single ϳ28 kDa band in X. laevis CNS and rat cerebellum. Retrograde transport of peroxidase combined with immunohistochemistry demonstrated that somata, axons, and synaptic terminals of laryngeal motor neurons in nucleus (N.) IX-X of X. laevis are calbindin-positive. The number of calbindin-positive cells was compared in the intact and axotomized sides of N.IX-X of gonadectomized males that were either hormonally untreated or DHT-treated for 1 month. Although axotomy decreased the number of calbindin-positive cells by 86% in hormonally untreated males, the decrease was only 56% in DHT-treated animals. Compared with hormonally untreated animals, the number of calbindin-labeled cells in N.IX-X of DHT-treated males was increased in both the intact (14%) and axotomized sides (75%). We conclude that axotomy decreases and that DHT enhances calbindin immunoreactivity in N.IX-X. Axotomy-induced decrease in calbindin immunoreactivity precedes cell loss in N.IX-X and may impair the capacity of motor neurons to regulate cytoplasmic calcium. Androgen-mediated maintenance of calbindin expression is thus a candidate cellular mechanism for trophic maintenance of hormone target neurons.

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Localization of parvalbumin, calretinin, and calbindin D-28k in identified extraocular motoneurons and internuclear neurons of the cat

Pastor

et al. 1998

J. Comp. Neurol.

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Calcium-binding proteins have been shown to be excellent markers of specific neuronal populations. We aimed to characterize the expression of calcium-binding proteins in identified populations of the cat extraocular motor nuclei by means of immunohistochemistry against parvalbumin, calretinin, and calbindin D-28k. Abducens, medial rectus, and trochlear motoneurons were retrogradely labeled with horseradish peroxidase from their corresponding muscles. Oculomotor and abducens internuclear neurons were retrogradely labeled after horseradish peroxidase injection into either the abducens or the oculomotor nucleus, respectively. Parvalbumin staining produced the highest density of immunoreactive terminals in all extraocular motor nuclei and was distributed uniformly. Around 15-20% of the motoneurons were moderately stained with antibody against parvalbumin, but their axons were heavily stained, indicating an intracellular segregation of parvalbumin. Colchicine administration increased the number of parvalbumin-immunoreactive motoneurons to approximately 85%. Except for a few calbindin-immunoreactive trochlear motoneurons (1%), parvalbumin was the only marker of extraocular motoneurons. Oculomotor internuclear neurons identified from the abducens nucleus constituted a nonuniform population, because low percentages of the three types of immunostaining were observed, calbindin being the most abundant (28.5%). Other interneurons located within the boundaries of the oculomotor nucleus were mainly calbindin-immunoreactive. The medial longitudinal fascicle contained numerous parvalbumin- and calretinin-immunoreactive but few calbindin-immunoreactive axons. The majority of abducens internuclear neurons projecting to the oculomotor nucleus (80.7%) contained calretinin. Moreover, the distribution of calretinin-immunoreactive terminals in the oculomotor nucleus overlapped that of the medial rectus motoneurons and matched the anterogradely labeled terminal field of the abducens internuclear neurons. Parvalbumin immunostained 42% of the abducens internuclear neurons. Colocalization of parvalbumin and calretinin was demonstrated in adjacent semithin sections, although single-labeled neurons were also observed. Therefore, calretinin is proven to be a good marker of abducens internuclear neurons. From all of these data, it is concluded that parvalbumin, calretinin, and calbindin D-28k selectively delineate certain neuronal populations in the oculomotor system and constitute valuable tools for further analysis of oculomotor function under normal and experimental conditions.

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Immunohistochemical mapping of calcium-binding protein immunoreactivity in the rat central nervous system

Cited by 271 publications

References 23 publications

Calcium binding protein-like immunoreactivity labels the terminal field of nucleus laminaris of the barn owl

Calcium binding protein-like immunoreactivity labels the terminal field of nucleus laminaris of the barn owl

Androgen Mitigates Axotomy-Induced Decreases in Calbindin Expression in Motor Neurons

Localization of parvalbumin, calretinin, and calbindin D-28k in identified extraocular motoneurons and internuclear neurons of the cat

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