Immunohistochemical mapping of natriuretic peptide receptor-A in the brainstem of Macaca fascicularis

Abdelalim, Essam M.; Osman, A. H. K.; Takada, Tatsuyuki; Torii, Ryuzo; Tooyama, Ikuo

doi:10.1016/j.neuroscience.2006.12.062

Cited by 12 publications

(3 citation statements)

References 40 publications

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“…While all NPRs have been identified in brainstem trigeminal nuclei [32–34], little is known about the possible role of the natriuretic peptide system at TG level where nociceptive signals are transduced [35–37]. A recent clinical report has shown that BNP levels are raised in the jugular vein blood during a migraine attack [38].…”

Section: Introductionmentioning

confidence: 99%

B-Type Natriuretic Peptide-Induced Delayed Modulation of TRPV1 and P2X3 Receptors of Mouse Trigeminal Sensory Neurons

et al. 2013

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Important pain transducers of noxious stimuli are small- and medium-diameter sensory neurons that express transient receptor vanilloid-1 (TRPV1) channels and/or adenosine triphosphate (ATP)-gated P2X3 receptors whose activity is upregulated by endogenous neuropeptides in acute and chronic pain models. Little is known about the role of endogenous modulators in restraining the expression and function of TRPV1 and P2X3 receptors. In dorsal root ganglia, evidence supports the involvement of the natriuretic peptide system in the modulation of nociceptive transmission especially via the B-type natriuretic peptide (BNP) that activates the natriuretic peptide receptor-A (NPR-A) to downregulate sensory neuron excitability. Since the role of BNP in trigeminal ganglia (TG) is unclear, we investigated the expression of BNP in mouse TG in situ or in primary cultures and its effect on P2X3 and TRPV1 receptors of patch-clamped cultured neurons. Against scant expression of BNP, almost all neurons expressed NPR-A at membrane level. While BNP rapidly increased cGMP production and Akt kinase phosphorylation, there was no early change in passive neuronal properties or responses to capsaicin, α,β-meATP or GABA. Nonetheless, 24 h application of BNP depressed TRPV1 mediated currents (an effect blocked by the NPR-A antagonist anantin) without changing responses to α,β-meATP or GABA. Anantin alone decreased basal cGMP production and enhanced control α,β-meATP-evoked responses, implying constitutive regulation of P2X3 receptors by ambient BNP. These data suggest a slow modulatory action by BNP on TRPV1 and P2X3 receptors outlining the role of this peptide as a negative regulator of trigeminal sensory neuron excitability to nociceptive stimuli.

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Section: Introductionmentioning

confidence: 99%

B-Type Natriuretic Peptide-Induced Delayed Modulation of TRPV1 and P2X3 Receptors of Mouse Trigeminal Sensory Neurons

et al. 2013

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“…Our previous report demonstrated the co-localization of NPR receptors (NPR-A, NPR-B and NPR-C) with ChAT-positive neurons in the central nervous system (Abdelalim et al, 2007, 2008a,b; Abdelalim and Tooyama, 2010). The relationship between NPs and cholinergic (ChAT-positive) neurons has been previously reported.…”

Section: Discussionmentioning

confidence: 92%

“…Several studies showed that BNP plays an essential role in cardiovascular homeostasis (Woodard and Rosado, 2007; Potter et al, 2009). However, other reports demonstrated that BNP and its receptors are expressed in several cell types that are not related to cardiovascular control, indicating BNP involvement in several functions (Cameron et al, 1996; Suda et al, 1998; Abdelalim et al, 2007, 2008a,b, 2013; Cao and Yang, 2008; Abdelalim and Tooyama, 2009, 2011a,b). …”

Section: Introductionmentioning

confidence: 99%

Localization of Brain Natriuretic Peptide Immunoreactivity in Rat Spinal Cord

Abdelalim¹,

Bellier²,

Tooyama³

2016

Front. Neuroanat.

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Brain natriuretic peptide (BNP) exerts its functions through NP receptors. Recently, BNP has been shown to be involved in a wide range of functions. Previous studies reported BNP expression in the sensory afferent fibers in the dorsal horn (DH) of the spinal cord. However, BNP expression and function in the neurons of the central nervous system are still controversial. Therefore, in this study, we investigated BNP expression in the rat spinal cord in detail using reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. RT-PCR analysis showed that BNP mRNA was present in the spinal cord and dorsal root ganglion (DRG). BNP immunoreactivity was observed in different structures of the spinal cord, including the neuronal cell bodies and neuronal processes. BNP immunoreactivity was observed in the DH of the spinal cord and in the neurons of the intermediate column (IC) and ventral horn (VH). Double-immunolabeling showed a high level of BNP expression in the afferent fibers (laminae I–II) labeled with calcitonin gene-related peptide (CGRP), suggesting BNP involvement in sensory function. In addition, BNP was co-localized with CGRP and choline acetyltransferase (ChAT) in the motor neurons of the VH. Together, these results indicate that BNP is expressed in sensory and motor systems of the spinal cord, suggesting its involvement in several biological actions on sensory and motor neurons via its binding to NP receptor-A (NPR-A) and/or NP receptor-B (NPR-B) at the spinal cord level.

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Mapping of NPR-B immunoreactivity in the brainstem of Macaca fascicularis

Abdelalim

Tooyama

2011

Brain Struct Funct

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C-type natriuretic peptide (CNP), the most abundant natriuretic peptide hormone in the brain, plays an important role in neuroendocrine function. The physiological effects of CNP are mediated by the natriuretic peptide receptor-B (NPR-B). Although CNP and NPR-B have been detected in several brain regions, little is known about the neuroanatomical localization of NPR-B protein in the brainstem. In the present study, we investigated the topographical distribution of NPR-B immunoreactivity in the monkey brainstem. The data demonstrate widespread NPR-B immunoreactivity throughout the brainstem. NPR-B immunoreactivity was located in the superior colliculus, inferior colliculus, periaqueductal gray, oculomotor nucleus, red nucleus, ventral tegmental area, substantia nigra, and cerebral peduncle of the midbrain, as well as in the abducens nucleus, medial vestibular nucleus, lateral vestibular nucleus, parabrachial nucleus, locus coeruleus, trigeminal motor nucleus, pontine reticular nucleus, facial nucleus, oral part of the spinal trigeminal nucleus, cochlear nucleus, raphe magnus nucleus, raphe pallidus nucleus, pontine nucleus of the pons, the dorsal motor nucleus of the vagus, hypoglossal nucleus, nucleus tractus solitarius, gracile nucleus, cuneate nucleus, medial vestibular nucleus, spinal trigeminal nucleus, nucleus ambiguus, lateral paragigantocellular nucleus, lateral reticular nucleus, and the inferior olivary nucleus of the medulla oblongata. The widespread distribution of NPR-B-immunoreactive structures throughout the monkey brainstem indicates that CNP may be involved in several physiological mechanisms, acting as a neurotransmitter and/or neuromodulator.

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Immunohistochemical mapping of natriuretic peptide receptor-A in the brainstem of Macaca fascicularis

Cited by 12 publications

References 40 publications

B-Type Natriuretic Peptide-Induced Delayed Modulation of TRPV1 and P2X3 Receptors of Mouse Trigeminal Sensory Neurons

B-Type Natriuretic Peptide-Induced Delayed Modulation of TRPV1 and P2X3 Receptors of Mouse Trigeminal Sensory Neurons

Localization of Brain Natriuretic Peptide Immunoreactivity in Rat Spinal Cord

Mapping of NPR-B immunoreactivity in the brainstem of Macaca fascicularis

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