Immunohistochemical mapping of neurotensin in the alpaca diencephalon

Sánchez, Manuel Lisardo; Mangas, A.; Medina, L.E.; Aguilar, Luís Fuentes; Dı́az-Cabiale, Zaida; Narváez, José Ángel; Coveñas, Rafael

doi:10.5603/fhc.a2018.0003

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…Using a freezing microtome, 40-45 µm-thick brain coronal sections were obtained and processed for immunocytochemistry. [8][9][10] As previously described [8][9][10]30,31 and in order to inactivate the endogenous peroxidases, sections were pre-incubated in a mixture solution of methanol/H 2 O 2 (2v/1v) for 30 min. Then, sections were washed three times with PBS for 30 min and pre-incubated in a PBS solution containing 0.3% Triton X-100 and supplemented with 1% of normal horse serum (mixture solution) for 30 min.…”

Section: Immunocytochemical Studymentioning

confidence: 99%

Overexpression of kynurenic acid and 3-hydroxyanthranilic acid after rat traumatic brain injury

et al. 2018

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Using an immunohistochemical technique, we have studied the distribution of kynuneric acid (KYNA) and 3-hydroxyanthranilic acid (3-HAA) in a rat brain injury model (trauma). The study was carried out inducing a cerebral ablation of the frontal motor cortex. Two mouse monoclonal specific antibodies previously developed by our group directed against KYNA and 3-HAA were used. In control animals (sham-operated), the expression of both KYNA and 3-HAA was not observed. In animals in which the ablation was performed, the highest number of immunoreactive cells containing KYNA or 3-HAA was observed in the region surrounding the lesion and the number of these cells decreased moving away from the lesion. KYNA and 3-HAA were also observed in the white matter (ipsilateral side) located close to the injured region and in some cells placed in the white matter of the contralateral side. The distribution of KYNA and 3-HAA perfectly matched with the peripheral injured regions. The results found were identical independently of the perfusion date of animals (17, 30 or 54 days after brain injury). For the first time, the presence of KYNA and 3-HAA has been described in a rat trauma model. Moreover, by using a double immunocytochemistry protocol, it has been demonstrated that both metabolites were located in astrocytes. The findings observed suggest that, in cerebral trauma, KYNA and 3-HAA are involved in tissue damage and that these compounds could act, respectively, as a neuroprotector and a neurotoxic. This means that, in trauma, a counterbalance occurs and that a regulation of the indoleamine 2,3 dioxygenase (IDO) pathway could be required after a brain injury in order to decrease the deleterious effects of ending metabolites (the neurotoxic picolinic acid). Moreover, the localization of KYNA and 3-HAA in the contralateral side of the lesion suggests that the IDO pathway is also involved in the sprouting and pathfinding that follows a traumatic brain injury.

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Section: Immunocytochemical Studymentioning

confidence: 99%

Overexpression of kynurenic acid and 3-hydroxyanthranilic acid after rat traumatic brain injury

et al. 2018

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“…In addition to those works focused on the alpaca reproductive mechanisms, several studies have been previously performed on the neuroanatomical location of neuropeptides (calcitonin gene-related peptide, somatostatin-28 (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12), neurotensin, leucine-enkephalin, alphaneo-endorphin, adrenocorticotropic hormone (18-39), beta-endorphin ) in the alpaca brainstem [17][18][19][20][21][22]. In addition, a recent study has shown a close neuroanatomical relationship between the enkephalinergic (methionine-enkephalin) and tachykininergic (substance P) systems in the alpaca diencephalon and the presence of some neuropeptides (e.g., neurotensin) has also been reported in this region of the alpaca CNS [5,23]. To our best knowledge no immunohistochemical study on the distribution of SP and MET has been carried out in the alpaca brainstem.…”

Section: Introductionmentioning

confidence: 99%

Neuroanatomical distribution of the enkephalinergic and tachykininergic systems in the alpaca brainstem: an immunohistochemical study

Sánchez

Mangas

et al. 2021

Folia Histochem Cytobiol.

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Introduction.A recent study has shown a close neuroanatomical relationship between the enkephalinergic (methionine-enkephalin) and tachykininergic (substance P) systems in the alpaca diencephalon. In this study, our aim is to show this relationship in the alpaca brainstem.Material and methods. Using an immunohistochemical technique, the distribution of immunoreactive (Ir) fibers and cell bodies containing substance P (SP) or methionineenkephalin (MET) has been studied in the alpaca brainstem. Five adult males were used; brain tissue was fixed and processed by standard methods.Results. SP-and MET-Ir fibers showed a widespread and similar distribution in the mesencephalon, pons and medulla oblongata. The co-localization of fibers containing SP or MET was found in most of the nuclei/tracts of the alpaca brainstem. This close neuroanatomical relationship suggests multiple physiological interactions between both neuropeptides. The distribution of the cell bodies containing SP was very restricted (cell bodies were only observed in a few nuclei located in the mesencephalon and medulla oblongata), whereas MET-Ir perikarya showed a moderately widespread distribution in the mesencephalon, pons and medulla oblongata. Conclusions.This study increases the knowledge on the neuroanatomical distribution/relationship of the tachykinergic (SP) and enkephalinergic (MET) systems in the alpaca central nervous system.

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“…Since 2007, the immunohistochemical mapping of several neuropeptides has been performed in the alpaca ( Lama pacos ) brainstem and diencephalon (Coveñas et al., , ; Manso et al., ; Marcos, Arroyo‐Jiménez, Lozano, Aguilar, & Coveñas, ; Marcos et al., ; Sánchez et al., ; de Souza, Sánchez, Aguilar, Díaz‐Cabiale, & Narváez, ; de Souza et al., , , ). In the brainstem of this species, the distribution of fibres/cell bodies containing leucine‐enkephalin, calcitonin gene‐related peptide, neurotensin or somatostatin 28 (1–12) is currently known (Marcos et al., ; de Souza et al., , , , ).…”

Section: Introductionmentioning

confidence: 99%

Distribution of alpha‐neoendorphin, ACTH (18–39) and beta‐endorphin (1–27) in the alpaca brainstem

Sánchez

Souza

Aguilar

et al. 2018

Anat Histol Embryol

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Using an immunocytochemical technique, we have studied in the alpaca brainstem the distribution of immunoreactive structures containing prodynorphin (alpha-neoendorphin)- and pro-opiomelanocortin (adrenocorticotrophin hormone (18-39) (ACTH), beta-endorphin (1-27))-derived peptides. No peptidergic-immunoreactive cell body was observed. Immunoreactive fibres were widely distributed, although in most of the brainstem nuclei the density of the peptidergic fibres was low or very low. In general, the distribution of the immunoreactive fibres containing the peptides studied was very similar. A close anatomical relationship occurred among the fibres containing alpha-neoendorphin, ACTH or beta-endorphin (1-27), suggesting a functional interaction among the three peptides in many of the brainstem nuclei. The number of fibres belonging to the prodynorphin system was higher than that of the pro-opiomelanocortin system. A moderate/low density of immunoreactive fibres was observed in 65.11% (for alpha-neoendorphin (1-27)), 18.18% (for ACTH) and 13.95% (for beta-endorphin) of the brainstem nuclei/tracts. In the alpaca brainstem, a high density of immunoreactive fibres was not observed. The neuroanatomical distribution of the immunoreactive fibres suggests that the peptides studied are involved in auditory, motor, gastric, feeding, vigilance, stress, respiratory and cardiovascular mechanisms, taste response, sleep-waking cycle and the control of pain transmission.

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Immunohistochemical mapping of neurotensin in the alpaca diencephalon

Abstract: In comparison with other mammals, the thalamus of the alpaca showed the most widespread distribution of neurotensin-immunoreactive fibers. The widespread distribution of neurotensin through the alpaca diencephalon suggests that the peptide can be involved in many physiological actions.

Cited by 3 publications

References 27 publications

Overexpression of kynurenic acid and 3-hydroxyanthranilic acid after rat traumatic brain injury

Overexpression of kynurenic acid and 3-hydroxyanthranilic acid after rat traumatic brain injury

Neuroanatomical distribution of the enkephalinergic and tachykininergic systems in the alpaca brainstem: an immunohistochemical study

Distribution of alpha‐neoendorphin, ACTH (18–39) and beta‐endorphin (1–27) in the alpaca brainstem

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