Immunohistochemical mapping of pro-opiomelanocortin- and pro-dynorphin-derived peptides in the alpaca (Lama pacos) diencephalon

Manso, Begoña; Sánchez, Manuel Lisardo; Medina, L.E.; Aguilar, Luís Fuentes; Dı́az-Cabiale, Zaida; Narváez, José Ángel; Coveñas, Rafael

doi:10.1016/j.jchemneu.2014.06.001

Cited by 6 publications

(42 citation statements)

References 27 publications

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“…In this latter study, no double‐labelled cell bodies were detectable, since tyrosine hydroxylase and somatostatinergic neuronal populations showed different morphologies and locations in the diencephalic regions. However, immunoreactive fibers containing somatostatin‐28 (1‐12), calcitonin gene‐related peptide, leucine‐enkephalin, adrenocorticotropin hormone, beta‐endorphin (1‐27), alpha‐melanocyte‐stimulating hormone and alpha‐neo‐endorphin have been located in many diencephalic nuclei of the alpaca (Coveñas et al, ; Manso et al, ). This suggests a close anatomical relationship and a possible functional interaction between somatostatin‐28 (1‐12) and the above‐mentioned peptides.…”

Section: Discussionmentioning

confidence: 99%

“…Six male adult alpacas ( Lama pacos ) (Huacaya breed) (5–8 years; 70–80 kg) were obtained from the Cayetano Heredia Peruvian University (Faculty of Veterinary Medicine and Animal Sciences, Lima, Peru). As previously reported (Coveñas et al, ; Manso et al, ), the animals were maintained at 0 m (sea level) from birth to death under standard conditions of light (lights on at 06:00 h and off at 20:00 h) and temperature (26°C) and had free access to food and water.…”

Section: Methodsmentioning

confidence: 99%

“…As previously reported (Coveñas et al, , 2012de Souza et al, 2007de Souza et al, , 2008de Souza et al, , 2014Manso et al, 2014;Marcos et al, 2011Marcos et al, , 2013, the animals were deeply anaesthetized with ketamine (10 mg/kg) and xylazine (4 mg/kg) (both intravenously), heparinized, and per-fused via the carotid artery with 3 L of cold 0.9% NaCl. This pre-rinse was immediately followed by infusion of the fixative: 5 L of cold 4% paraformaldehyde in 0.15 M phosphate-buffered saline (PBS) (pH 7.2).…”

Section: Materials and Methods Animals And Tissue Preparationmentioning

confidence: 99%

“…Alpacas are able to live at sea level and at more than 5,000 m altitude, making this animal an interesting species that could reveal the existence of special and unique regulatory mechanisms. During the last eight years several papers regarding the distribution of neuropeptides in the camelid Lama pacos have been published (Coveñas et al, , 2012de Souza et al, 2007de Souza et al, , 2008de Souza et al, , 2014Manso et al, 2014;Marcos et al, 2011Marcos et al, , 2013. Thus, with the use of immunocytochemical techniques the distribution of fibers and cell bodies containing somatostatin-28 (1-12), calcitonin generelated peptide, adrenocorticotropin hormone, alphamelanocyte-stimulating hormone, beta-endorphin (1-27), alpha-neo-endorphin and leucine-enkephalin have been reported in the alpaca diencephalon (Coveñas et al, , 2012Manso et al, 2014;Marcos et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…During the last eight years several papers regarding the distribution of neuropeptides in the camelid Lama pacos have been published (Coveñas et al, , 2012de Souza et al, 2007de Souza et al, , 2008de Souza et al, , 2014Manso et al, 2014;Marcos et al, 2011Marcos et al, , 2013. Thus, with the use of immunocytochemical techniques the distribution of fibers and cell bodies containing somatostatin-28 (1-12), calcitonin generelated peptide, adrenocorticotropin hormone, alphamelanocyte-stimulating hormone, beta-endorphin (1-27), alpha-neo-endorphin and leucine-enkephalin have been reported in the alpaca diencephalon (Coveñas et al, , 2012Manso et al, 2014;Marcos et al, 2013). Regarding the knowledge of the chemical neuroanatomy of neuropeptides in the alpaca brainstem, the distribution of fibers and perikarya containing leucineenkephalin, calcitonin gene-related peptide and neurotensin has been studied (de Souza et al, 2007(de Souza et al, , 2008(de Souza et al, , 2014Marcos et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Mapping of somatostatin‐28 (1‐12) in the alpaca (Lama pacos) brainstem

Souza

Sánchez

Aguilar

et al. 2015

Microscopy Res & Technique

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Using an indirect immunoperoxidase technique, we studied the distribution of cell bodies and fibers containing somatostatin-28 (1-12) in the alpaca brainstem. Immunoreactive fibers were widely distributed throughout the whole brainstem: 34 brainstem nuclei/regions showed a high or a moderate density of these fibers. Perikarya containing the peptide were widely distributed throughout the mesencephalon, pons and medulla oblongata. Cell bodies containing somatostatin-28 (1-12) were observed in the lateral and medial divisions of the marginal nucleus of the brachium conjunctivum, reticular formation (mesencephalon, pons and medulla oblongata), inferior colliculus, periaqueductal gray, superior colliculus, pericentral division of the dorsal tegmental nucleus, interpeduncular nucleus, nucleus of the trapezoid body, vestibular nucleus, motor dorsal nucleus of the vagus, nucleus of the solitary tract, nucleus praepositus hypoglossi, and in the substantia nigra. This widespread distribution indicates that somatostatin-28 (1-12) is involved in multiple physiological actions in the alpaca brainstem.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Materials and Methods Animals And Tissue Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Mapping of somatostatin‐28 (1‐12) in the alpaca (Lama pacos) brainstem

Souza

Sánchez

Aguilar

et al. 2015

Microscopy Res & Technique

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Mapping of enkephalins and adrenocorticotropic hormone in the squirrel monkey brainstem

et al. 2016

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An immunocytochemical technique has been used to study for the first time the distribution of fibers and cell bodies containing leucine-enkephalin (leu-enk), methionine-enkephalin (met-enk) or adrenocorticotropic hormone (ACTH) in the whole brainstem of the squirrel monkey Saimiri sciureus. Cell bodies containing leu-enk or met-enk were found in the superior colliculus and the formatio reticularis tegmenti mesencephali, respectively. No immunoreactive cell bodies containing ACTH were observed. Leu-enk-immunoreactive fibers were observed in 40 brainstem nuclei/tracts/regions, fibers containing met-enk were found in 38 brainstem nuclei/tracts/regions and fibers containing ACTH were found in 26 nuclei/tracts/regions. In the latter case, the density of immunoreactive fibers was always low. A high/moderate density of leu-enk- or met-enk-immunoreactive fibers were found in 18 and 16 brainstem nuclei/tracts/regions, respectively. The distribution of immunoreactive fibers containing leu-enk or met-enk was quite similar, with both leu-enk and met-enk observed in 82.5 % of the squirrel monkey brainstem nuclei/tracts/regions. This relationship is less marked for met-enk and ACTH (60.5 %) and even lower for leu-enk and ACTH (52.5 %). In 42.5 % of the nuclei/tracts/regions of the squirrel monkey brainstem (colliculus superior, substantia grisea centralis, nucleus interpeduncularis, nucleus tractus spinalis nervi trigemini, nucleus tractus solitarii, nucleus parabrachialis, formatio reticularis, substantia nigra), we observed fibers containing all three neuropeptides. The widespread distribution reported here suggests that enkephalins and ACTH can be involved in several physiological functions. The distribution of the immunoreactive fibers reported here is quite similar to that previously reported for enkephalins and ACTH in Macaca species and humans.

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