Immunohistochemical observation of growth-associated protein 43 (GAP-43) in the developing circumvallate papilla of the rat

Wakisaka, Satoshi; Daikoku, Hiroshi; Miyawaki, Y.; Youn, Suk Hyun; Kurisu, Kojiro; Maeda, Takeyasu

doi:10.1007/s004410051142

Cited by 15 publications

(15 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar pattern was demonstrated in the vallate papilla (AhPin et al, 1989;Hirata and Kanaseki, 1989;Wakisaka et al, 1996Wakisaka et al, , 1998Mbiene and Roberts, 2003). However, in contrast to NP, there was drastic shortfall in taste buds in the apical epithelium with few remaining in the adult vallate papilla.…”

supporting

confidence: 74%

Immunohistochemical distribution of growth‐associated protein 43 (GAP‐43) in developing rat nasoincisor papilla

2004

View full text Add to dashboard Cite

We employed immunohistochemistry of growth-associated protein 43 (GAP-43) to trace the early development of gustatory nerves and ␣-gustducin to demonstrate mature taste buds in the rat nasoincisor papilla (NP). The sequential changes of gustatory structures revealed eight characteristic stages. One, at embryonic day 16 (E16), GAP-43-immunoreactive (IR) nerve fibers were observed in close relation with presumptive taste buds in the lateral apical epithelium on each side of NP; meanwhile, no immunoreactivity could be observed in the papillary epithelium. Two, at E17, fine GAP-43-IR nerve fibers first invaded the apical epithelium of the papilla. Three, at E19, GAP-43-IR nerve fibers were extensive in apical epithelium and colonized in immature taste buds. Four, at E20, GAP-43-IR nerve fibers were first observed in ductal epithelium (lining the medial wall of nasoincisor ducts). Five, at postnatal day 1 (P1), immunoreactive nerve fibers first coincided with immature taste buds in the ductal epithelium. Six, at P3, ␣-gustducin-IR cells identical for mature taste buds were simultaneously demonstrated in both apical and ductal epithelium. Seven, at P14, progressive taste bud proliferation and maturation as well as neural invasion were demonstrated in all regions of the epithelium. Eight, during advanced stage in adult animals, extensive innervation was traced especially in close relation with taste buds. The sequential topographic patterns of NP gustatory structures seem very specific as compared to those in other locations of mammalian gustatory system. The present study reveals that gustatory nerves preceded the development of taste buds. However Key words: innervation; gustatory epithelium; neural inductionMammalian taste buds are distributed throughout the oropharyngeal epithelium most likely within papillary structures such as vallate, fungiform, foliate, and nasoincisor papillae, while they are diffusely scattered among the soft palatal and laryngeal epithelium (Miller, 1977;Miller and Smith, 1984;Belecky and Smith, 1990). Previous publications revealed that the temporal and spatial patterns of taste buds differ according to their locations and their source of innervation (Belecky and Smith, 1990). Briefly, the fungiform and soft palate taste buds that are innervated by the seventh cranial nerve (facial nerve) develop mostly during prenatal life, while for those innervated by the ninth (glossopharyngeal nerve) cranial nerve such as vallate and foliate or the tenth cranial nerve (vagus nerve), laryngeal taste buds appear at later stages of development. In a previous report, we found the taste

show abstract

supporting

confidence: 74%

Immunohistochemical distribution of growth‐associated protein 43 (GAP‐43) in developing rat nasoincisor papilla

2004

View full text Add to dashboard Cite

show abstract

“…It is a good marker for fine peripheral nerve fibers and has been extensively used for the study of lingual and gustatory papillae innervation (AhPin et al 1989; Wakisaka et al 1996Wakisaka et al , 1998Mbiene and Mistretta 1997;Ringstedt et al 1999). Wakisaka et al (1996) reported the presence of PGP 9.5-IR nerve fibers and neurons in the vallate papilla in the developing mouse.…”

Section: Discussionmentioning

confidence: 99%

The distribution of PGP9.5, BDNF and NGF in the vallate papilla of adult and developing mice

Chou¹,

Chien²,

Lu³

2001

Anatomy and Embryology

View full text Add to dashboard Cite

The development and innervation of vallate papillae and taste buds in mice were studied using antibodies against the neuronal marker, protein gene product 9.5 (PGP 9.5), and against nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). PGP 9.5 immunohistochemical studies revealed that the earliest sign of median vallate papilla formation was an epithelial bulge at embryonic day 13 (E13), and at E14, a dense nerve plexus was found within the connective tissue core of the papilla. Thin nerve fibers penetrated the apical and medial trench wall epithelium of the papilla at E16 and a few of these began to invade the lateral trench wall epithelium at E17. At postnatal day 1 (P1), the newly formed taste buds were recognizable and a small number of PGP 9.5-immunoreactive (IR) cells appeared on the medial trench wall epithelium. The number of PGP 9.5-IR taste bud cells then increased gradually and reached the adult level at postnatal week 2. PGP 9.5 immunoreactivity increased systematically with age. NGF and BDNF immunoreactivity was first seen at the boundary between the columnar cells in the apical epithelium of the developing vallate papilla at E13, then in the medial and lateral trench walls at E15 (BDNF) or E18 (NGF). At P1, BDNF immunoreactivity was exclusively present in the newly formed taste buds of the medial trench wall. The number of BDNF-IR taste bud cells then increased gradually, reaching the adult level at P7. Similar degrees of NGF and BDNF immunoreactivity were seen in the developing vallate papilla. In the present study, we found that the vallate papilla was formed prior to its innervation, and we propose that initiation of papilla formation does not require any direct influence from the specific gustatory nerve. We also suggest that neurotrophins in the early developing vallate papillae might act as local tropic factors for the embryonic growth of nerve fibers to induce differentiation of the taste buds.

show abstract

“…Taste buds/taste bud progenitors were visualized by an antibody to cytokeratin 8 (Troma-1; Knapp et al 1995;Zhang et al 1995). The antibody to GAP-43 was used to assess innervation (Mistretta and Haus 1996;Wakisaka et al 1998;Ringstedt et al 1999). Samples fixed in 10% acetic acid in 70% ethanol were incubated with antibodies against Troma-1 (1:80, Hybridoma Bank, Iowa City, Iowa, USA) and GAP-43 (1:200, Chemicon, Temecula, Calif., USA) for 48-72 h at 4°C, rinsed in PBS, and incubated at room temperature for 2 h with Alexa-488-conjugated anti-rabbit IgG (1:400; Molecular Probes, Eugene, Ore., USA) and Alexa-594-conjugated anti-rat IgG (1:400; Molecular Probes).…”

Section: Methodsmentioning

confidence: 99%

Gustatory papillae and taste bud development and maintenance in the absence of TrkB ligands BDNF and NT-4

Ito

Nosrat

2009

Cell Tissue Res

View full text Add to dashboard Cite

Taste buds and the peripheral nerves innervating them are two important components of the peripheral gustatory system. They require appropriate connections for the taste system to function. Neurotrophic factors play crucial roles in the innervation of peripheral sensory organs and tissues. Both brain-derived neurotrophic factor (BDNF) null-mutated and neurotrophin-4 (NT-4) null-mutated mice exhibit peripheral gustatory deficits. BDNF and NT-4 bind to a common high affinity tyrosine kinase receptor, TrkB (NTRK-2), and a common p75 neurotrophin receptor (NGFR). We are currently using a transgenic mouse model to study peripheral taste system development and innervation in the absence of both TrkB ligands. We show that taste cell progenitors express taste cell markers during early stages of taste bud development in both BDNF(-/-)xNT-4(-/-) and wild-type mice. At early embryonic stages, taste bud progenitors express Troma-1, Shh, and Sox2 in all mice. At later stages, lack of innervation becomes a prominent feature in BDNF(-/-)xNT-4(-/-) mice leading to a decreasing number of fungiform papillae and morphologically degenerating taste cells. A total loss of vallate taste cells also occurs in postnatal transgenic mice. Our data indicate an initial independence but a later permissive and essential role for innervation in taste bud development and maintenance.

show abstract

Immunohistochemical observation of growth-associated protein 43 (GAP-43) in the developing circumvallate papilla of the rat

Cited by 15 publications

References 45 publications

Immunohistochemical distribution of growth‐associated protein 43 (GAP‐43) in developing rat nasoincisor papilla

Immunohistochemical distribution of growth‐associated protein 43 (GAP‐43) in developing rat nasoincisor papilla

The distribution of PGP9.5, BDNF and NGF in the vallate papilla of adult and developing mice

Gustatory papillae and taste bud development and maintenance in the absence of TrkB ligands BDNF and NT-4

Contact Info

Product

Resources

About