Immunohistochemical profile of stromal constituents and lymphoid cells over the course of wound healing in murine model

Almeida, Bruno; Nascimento, Marismar Fernandes do; Pereira-Filho, Rose Nely; Melo, Genecy Calado de; Santos, José Ribeiro dos; Oliveira, Clauberto Rodrigues de; Gomes, Margarete Zanardo; Lima, Sônia Oliveira; Albuquerque‐Júnior, Ricardo Luiz Cavalcanti de

doi:10.1590/s0102-8650201400150007

Cited by 12 publications

(10 citation statements)

References 19 publications

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“…Myofibroblasts begin to differentiate in the wound area from the third day after the initial injury [ 37 ], which justifies the low wound retraction rates on day 3. The peak of myofibroblast differentiation, in a murine model of second intention wound healing, occurs around day 7, at which point there is a progressive reduction in this cell population [ 38 ], as also observed in the present study. As GelMA-HB presented a significantly higher WCR over the course of wound healing until day 14, the hypothesis that the phytochemical components found in EEHB could have accelerated or intensified myofibroblast differentiation in vivo was brought up and analyzed.…”

Section: Discussionsupporting

confidence: 78%

Himatanthus bracteatus-Composed In Situ Polymerizable Hydrogel for Wound Healing

Almeida

Santos

Carvalho

et al. 2022

IJMS

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The Himatanthus genus presents anti-inflammatory, antioxidant activities, suggesting potential wound-healing properties. This study aimed to develop and analyze the wound-healing properties of a photopolymerizable gelatin-based hydrogel (GelMA) containing an ethanolic extract of Himatanthus bracteatus in a murine model. The extract was obtained under high pressure conditions, incorporated (2%) into the GelMA (GelMA-HB), and physically characterized. The anti-inflammatory activity of the extract was assessed using a carrageenan-induced pleurisy model and the GelMA-HB scarring properties in a wound-healing assay. The extract reduced IL-1β and TNF-α levels (48.5 ± 6.7 and 64.1 ± 4.9 pg/mL) compared to the vehicle (94.4 ± 2.3 pg/mL and 106.3 ± 5.7 pg/mL; p < 0.001). GelMA-HB depicted significantly lower swelling and increased resistance to mechanical compression compared to GelMA (p < 0.05). GelMA-HB accelerated wound closure over the time course of the experiment (p < 0.05) and promoted a significantly greater peak of myofibroblast differentiation (36.1 ± 6.6 cells) and microvascular density (23.1 ± 0.7 microvessels) on day 7 in comparison to GelMA (31.9 ± 5.3 cells and 20.2 ± 0.6 microvessels) and the control (25.8 ± 4.6 cells and 17.5 ± 0.5 microvessels) (p < 0.05). In conclusion, GelMA-HB improved wound healing in rodents, probably by modulating the inflammatory response and myofibroblastic and microvascular differentiation.

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Section: Discussionsupporting

confidence: 78%

Himatanthus bracteatus-Composed In Situ Polymerizable Hydrogel for Wound Healing

Almeida

Santos

Carvalho

et al. 2022

IJMS

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“…For the collagen abundance, our data show a non-significant decreasing trend from postwounding day 3 to day 7 and an increasing trend thereafter. Majority of the studies which investigated the time course changes in collagen using either diabetic (Ponrasu and Suguna, 2012;Ram et al, 2015;Zhang et al, 2016) or non-diabetic wound models (Oxlund et al, 1996;Ponrasu and Suguna, 2012;Almeida et al, 2014) demonstrated an increase in collagen abundance over time and plateau or slightly decreases in the very late phase, which is consistent with the rising trend from day 7 to day 21 concluded in the current study. Therefore, the nonsignificant decrease from day 3 to day 7 in the collagen abundance shown in the present study could be attributed to individual variation.…”

Section: Discussionsupporting

confidence: 89%

Indentation Stiffness Measurement by an Optical Coherence Tomography-Based Air-Jet Indentation System Can Reflect Type I Collagen Abundance and Organisation in Diabetic Wounds

Choi

Cheung

et al. 2021

Front. Bioeng. Biotechnol.

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There is a lack of quantitative and non-invasive clinical biomechanical assessment tools for diabetic foot ulcers. Our previous study reported that the indentation stiffness measured by an optical coherence tomography-based air-jet indentation system in a non-contact and non-invasive manner may reflect the tensile properties of diabetic wounds. As the tensile properties are known to be contributed by type I collagen, this study was aimed to establish the correlations between the indentation stiffness, and type I collagen abundance and organisation, in order to further justify and characterise the in vivo indentation stiffness measurement in diabetic wounds. In a male streptozotocin-induced diabetic rat model, indentation stiffness, and type I collagen abundance and organisation of excisional wounds were quantified and examined using the optical coherence tomography-based air-jet indentation system and picrosirius red polarised light microscopy, respectively, on post-wounding days 3, 5, 7, 10, 14, and 21. The results showed significant negative correlations between indentation stiffness at the wound centre, and the collagen abundance and organisation. The correlations between the indentation stiffness, as well as collagen abundance and organisation of diabetic wounds suggest that the optical coherence tomography-based air-jet indentation system can potentially be used to quantitatively and non-invasively monitor diabetic wound healing in clinical settings, clinical research or preclinical research.

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“…Wound contraction was analysed using antibodies against α-SMA, a marker of myofibroblast differentiation, as shown in Figure 6C [30]. In keeping with the rest of the results, after analysing the percentage of stained area, no differences were found among the groups (Figure 6D).…”

Section: Resultsmentioning

confidence: 56%

Development of Bioinspired Gelatin and Gelatin/Chitosan Bilayer Hydrofilms for Wound Healing

et al. 2019

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In the current study, we developed a novel gelatin-based bilayer wound dressing. We used different crosslinking agents to confer unique properties to each layer, obtaining a bioinspired multifunctional hydrofilm suitable for wound healing. First, we produced a resistant and non-degradable upper layer by lactose-mediated crosslinking of gelatin, which provided mechanical support and protection to overall design. For the lower layer, we crosslinked gelatin with citric acid, resulting in a porous matrix with a great swelling ability. In addition, we incorporated chitosan into the lower layer to harness its wound healing ability. FTIR and SEM analyses showed that lactose addition changed the secondary structure of gelatin, leading to a more compact and smoother structure than that obtained with citric acid. The hydrofilm was able to swell 384.2 ± 57.2% of its dry weight while maintaining mechanical integrity. Besides, its water vapour transmission rate was in the range of commercial dressings (1381.5 ± 108.6 g/m2·day). In vitro, cytotoxicity assays revealed excellent biocompatibility. Finally, the hydrofilm was analysed through an ex vivo wound healing assay in human skin. It achieved similar results to the control in terms of biocompatibility and wound healing, showing suitable characteristics to be used as a wound dressing.

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Immunohistochemical profile of stromal constituents and lymphoid cells over the course of wound healing in murine model

Cited by 12 publications

References 19 publications

Himatanthus bracteatus-Composed In Situ Polymerizable Hydrogel for Wound Healing

Himatanthus bracteatus-Composed In Situ Polymerizable Hydrogel for Wound Healing

Indentation Stiffness Measurement by an Optical Coherence Tomography-Based Air-Jet Indentation System Can Reflect Type I Collagen Abundance and Organisation in Diabetic Wounds

Development of Bioinspired Gelatin and Gelatin/Chitosan Bilayer Hydrofilms for Wound Healing

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