Immunohistochemical profile of various neurotransmitters, neurotrophins and MIB-1 in cholesteatomas of the petrous bone

Artico, Marco; Bronzetti, Elena; Vasco, Vincenza Rita Lo; Ionta, Brunella; Alicino, Valentina; D'Ambrosio, A; Magliulo, Giuseppe

doi:10.3892/mmr.1.3.347

Cited by 3 publications

(4 citation statements)

References 19 publications

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“…[19][20][21][22] A previous study identified the presence of neurotrophins in cholesteatoma. 23 These signaling molecules are capable of stimulating immune-mediated cells, such as macrophages, involved in the reabsorption of the bony covering.…”

Section: Discussionmentioning

confidence: 99%

Facial Nerve Dehiscence and Cholesteatoma

Magliulo¹,

Colicchio²,

Appiani³

2011

Ann Otol Rhinol Laryngol

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Dehiscence of the facial nerve was found in 27.1% of patients with cholesteatoma, with a significant difference between patients of pediatric and adult ages. A dehiscent facial nerve was more commonly seen during revision surgery and more frequent in patients older than 16 years. The site of dehiscence most frequently involved by cholesteatoma was the tympanic segment. The presence of a semicircular canal fistula increases the risk of facial nerve dehiscence. Finally, the results of preoperative CT scans are encouraging for the use of CT in predicting facial nerve dehiscence.

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Section: Discussionmentioning

confidence: 99%

Facial Nerve Dehiscence and Cholesteatoma

Magliulo¹,

Colicchio²,

Appiani³

2011

Ann Otol Rhinol Laryngol

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“…As we previously described, 15 Ki67 is a cell proliferation marker, utilized as a prognostic factor in the follow up of many neoplastic lesions, therefore our purpose was to analyze the expression level of MIB-1 (anti-Ki67) in the Reinke's edema samples, with the purpose of evaluate its biological role in this disease and its diagnostic application.…”

Section: Introductionmentioning

confidence: 99%

Reinke’s Edema: investigations on the role of MIB-1 and hepatocyte growth factor

Artico¹,

Bronzetti²,

Ionta³

et al. 2010

Eur J Histochem

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Reinke's edema is a benign disease of the human vocal fold, which mainly affects the sub-epithelial layer of the vocal fold. Microscopic observations show a strongly oedematous epithelium with loosened intercellular junctions, a disruption of the extracellular connections between mucosal epithelium and connective tissue, closely adherent to the thyroarytenoid muscle. Thickening of the basal layer of epithelium, known as Reinke's space, high deposition of fibronectin and chronic inflammatory infiltration it is also visible. We analyzed, together with the hepatocyte growth factor (HGF), the expression level of MIB-1 in samples harvested from patients affected by Reinke's edema, in order to define its biological role and consider it as a possible prognostic factor in the follow-up after surgical treatment. We observed a moderate expression of HGF in the lamina propria of the human vocal fold and in the basal membrane of the mucosal epithelium. Our finding suggests that this growth factor acts as an anti - fibrotic agent in Reinke's space and affects the fibronectin deposition in the lamina propria. MIB-1, on the contrary, showed a weak expression in the basement membrane of the mucosal epithelium and a total absence in the lamina propria deep layer, thus suggesting that only the superficial layer is actively involved in the reparatory process with a high regenerative capacity, together with a high deposition of fibronectin. The latter is necessary for the cellular connections reconstruction, after the inflammatory infiltration.

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“…Since little is known about the pathological and histological differentiation among the benign and malignant forms of this tumor (malignancy is very rare), we decided to establish a panel of molecular factors, capable of better describing and clarifying this characterization. Therefore, as Yamamoto did previously (12), we investigated the expression of neurotrophins and their relative receptors in samples of benign jugulotympanic tumors and in normal and neoplastic non-neural tissues (19), to identify any direct involvement of these growth factors in the development of these neoplasms. In our surgical series, we selected intradural jugulotympanic tumors in order to analyse the clinical aggressiveness of these lesions comparing the proliferation rate of neutrophins expression and the incidence of recurrence.…”

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confidence: 98%

“…Although many pathologists include an assessment of MIB-l labeling in diagnostic profile of paragangliomas, there is currently no prospect of a standardization, and reporting a Ki-67 index still remains optional (18). Since we have previously described the role of MIB-l as a prognostic factor in other neoplasms (19), we decided to investigate this factor, supporting the results shown by Rodriguez, who first described (using immunohistochemical techniques) the presence and the localization of MIB-l in these tumors (8).…”

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confidence: 99%

Immunohistochemical Profile of Neurotrophins and MIB-1 in Jugulotympanic Paragangliomas: Prognostic Value and Review of the Literature

Artico

Ionta

et al. 2012

Int J Immunopathol Pharmacol

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Jugulo-tympanic paragangliomas are the most common primary neoplasm of the middle ear, but little is still known about the histological features differentiating the benign and malignant forms. We investigated, with an immunohistochemical procedure, the expression of neurotrophins with their receptors, in fifteen samples of paragangliomas, and MIB-1 in order to consider them as prognostic factors of malignancy. We observed a general positivity for NGF - TrKA - NT4 - TrKC in the cytoplasm, and a strong expression for BDNF in the extracellular space. MIB-1 was moderate in the nucleus of neoplastic cells, weak in the cytoplasm and totally absent in the extracellular space. The comparison between the clinical recurrences and the rate of cytoplasmatic neurotrophins showed strong immunoreactivity in recurrent patients. It should be emphasized that 2 of the 3 recurrences had a wider distribution of the neutrophins, leading to hypothesize the involvement of these substances in the cell proliferation of glomus tumors. Malignant forms of these rare glomus tumors cannot be clearly identified using MIB-1 as a prognostic marker, although we can affirm that neurotrophins and their receptors can be considered as a panel of potential diagnostic markers to monitor the development of such malignancies. Although the small number of patients does not allow definitive conclusions to be made, our findings showed a possible trend towards significance which requires a more powerful study to evaluate this further.

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Immunohistochemical profile of various neurotransmitters, neurotrophins and MIB-1 in cholesteatomas of the petrous bone

Cited by 3 publications

References 19 publications

Facial Nerve Dehiscence and Cholesteatoma

Facial Nerve Dehiscence and Cholesteatoma

Reinke’s Edema: investigations on the role of MIB-1 and hepatocyte growth factor

Immunohistochemical Profile of Neurotrophins and MIB-1 in Jugulotympanic Paragangliomas: Prognostic Value and Review of the Literature

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