Immunohistochemical staining patterns of cytokeratins 13, 14, and 17 in oral epithelial dysplasia including orthokeratotic dysplasia

Abstract

Diagnosis of the exact grade of oral epithelial dysplasia is difficult, and interobserver variations in grading are common. The aim of this study was to investigate the expression patterns of cytokeratins (CKs) in dysplastic oral epithelia, to identify useful double immunostaining diagnostic markers. Immunoexpression of CK13, CK14, CK17, and Ki-67 were investigated in 21 normal epithelial specimens and 146 epithelial dysplasia specimens. In epithelial dysplasia specimens, orthokeratotic dysplasia (OKD) was identified using CK10 immunostaining. Most mild dysplasia specimens were CK13+ and CK17-. In moderate dysplasia, CK13 expression tended to be lower and CK17 expression tended to be higher than in mild dysplasia. All carcinoma in situ (CIS) specimens were CK17+. In differentiated type CIS specimens, CK13 expression was weakly positive. Most epithelial dysplasia specimens were CK14+. There were no significant differences in the expression patterns of CKs between OKD and non-OKD specimens in any of the grades of dysplasia. These results indicate that CK14 expression can be used to detect early epithelial dysplasia, and that CK13 and CK17 expression are useful for detecting neoplastic changes.

Tachykinin Receptor 3 Distribution in Human Oral Squamous Cell Carcinoma

“…CK-13 (type I cytokeratin) is an important component of mucosal-stratified squamous epithelium and CK-13-positive cells are present during normal differentiation and keratinization (18,19). In the present study, the expression of TACR3 was associated with CK-13-positive epithelial dysplasia, indicating that TACR3 may be involved in abnormal differentiation in dysplastic epithelia.…”

Intraepithelial CD163+ macrophages in tongue leukoplakia biopsy: A promising tool for cancer screening

“…Ki‐67, CK13 and CK17 are useful histopathological markers for the diagnosis or prognosis of oral squamous epithelium (Yagyuu et al, ; Kövesi & Szende ; Mikami et al, ; Nobusawa, Sano, Negishi, Yokoo, & Oyama, ; Ohta et al, ). However, we found no between‐group differences regarding these factors in the present study.…”

Multivariate analyses of Ki‐67, cytokeratin 13 and cytokeratin 17 in diagnosis and prognosis of oral precancerous lesions

“…Many studies have identified molecular markers for the pathological diagnosis or prognosis of oral atypical epithelium (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21). For example, immunohistochemical detection of the proliferation marker Ki-67 and the squamous cell-differentiation markers cytokeratin 13 (CK13) and CK17 were reported to be useful for differentiating between OPL and reactive atypical epithelium (2,6,9,10,(15)(16)(17)(18)(19)(20)(21). CK13 is usually expressed in the normal oral epithelium but not in OPL, whereas CK17 is expressed in OPL, but not the normal oral epithelium.…”

“…Overall, studies assessing the diagnostic or prognostic potential of molecular markers are limited. In these studies (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21), the reliability of immunohistochemical scoring methods, the rationale for selecting certain cutoff points for defining sensitivity or specificity, and ascertainment of the independent contributory role(s) of each molecular marker to diagnosis or prognosis have been controversial. In this study, we sought to assess the diagnostic values of Ki-67, CK13, and CK17 for high-grade dysplasia by using logistic regression analyses.…”