Immunohistochemical Study of Cell Proliferation in Hepatocellular Carcinoma

Abstract

Hepatocellular carcinoma (HCC), the most common primary liver cancer, with a poor prognosis, is the fifth most common cancer in men and the seventh in women. The study was made on 32 surgically removed liver carcinomas. In order to compare results, we included a group of non-tumor lesions obtained by liver biopsy. Assessment of the proliferative activity of the studied liver lesions was made using immunohistochemical stains with the monoclonal Ki-67 antibody, clone MIB-1 ready-to-use (DAKO Cytomation CA, USA), in the LSAB-HRP work system. To appreciate the proliferation index of Ki-67 (PI Ki-67), we used the semi-automated method of counting the nuclei on digital images. The statistical analysis was performed using SPSS software, Version 20.0 (IBM SPSS Statistics) and Microsoft Office Excel 2007. Mean value of Ki-67 index was 0.4 � 0.2 in normal liver, 3.52 � 0.2 in non-tumor liver lesions and 13.4�7.7 in HCC (p [ 0.001). In chronic hepatitis, PI Ki-67 varied between 2.5 and 5.8 %, with a mean value of 5.2% in portal chronic hepatitis and 5.5% in active chronic hepatitis with cirrhotic evolution. In HCC, the values of Ki-67 index were between 0.7% and 52%, with a mean of PI Ki-67 of 13.43�7.7. 66.6% of HCC associated with hepatitis B virus infection and those developped from a cirrhotic lesion had a high Ki-67 score (p = 0.1). The results we obtained showed: a low Ki-67 score in patients with well-differentiated HCC (G1) (p[0.001), with or without capsule infiltration (p = 0.003); high PI Ki-67 in 33.33% of HCC detected in the right hepatic lobe and those extended bilaterally at the moment of diagnosis (p = 0.142) and a significant relationship between high Ki-67 score and vascular invasion (p [ 0.001), the presence of intrahepatic metastasis being correlated with a high proliferative rate (p [ 0.001). Differences between the proliferation rate of HCC and non-tumor liver lesions (p [ 0.001) show that the uncontrolled division of tumor cells can play an important role in the developpment of HCC.

Succinic Ester‐Based Shape Memory Gelatin Sponge for Noncompressible Hemorrhage without Hindering Tissue Regeneration

“…[ 42 ] Ki67 positive cell number was very small in healthy livers, which is in accordance with previous reports ( Figure a1; and Figure S9, Supporting Information). [ 43,44 ] Ssponge‐2‐treated livers showed a significantly lower number of Ki67 positive cells than Csponge‐treated ones at all the time points (Figure 7a2). Since Ki67 level is associated with the cell cycle, the larger area of Ki67‐positive cells observed in Csponge‐treated livers, might indicate that the regeneration was still in the process even 14 and 21 days postsurgery.…”

Study on multidrug resistance mechanism and reversal strategy of hepatocellular carcinoma based on exosome

“…The results showed that wild-type p-53 transfection could play a synergistic or additive role with some drugs such as 5-FU, cisplatin and bleomycin. In clinical trials, literature [20] showed that among 24 patients with advanced NSCLC and abnormal p53, intratumoral injection of adenovirus-mediated p53(Adp53) combined with CDPP was effective in 17 cases.…”

The Diagnostic Value of Hepatocyte Paraffin Antibody 1 in Differentiating Hepatocellular Carcinoma from non-hepatic Tumors

“…Analyzing the possible prognostic value of Hep Par-1 in patients with surgically resected HCC, Mondada et al [21] finds Hep Par-1 expression, considering it a favorable prognostic factor in small size tumors. Otherwise, tumor size and growth has been already linked to multiple cellular and plasmatic markers hyper expression [22][23][24][25].…”