Immunohistochemical Study of Dark and Progenitor Carotid Body Cells: Artefacts or Real Subtypes?

Otlyga, Dmitry; Junemann, Olga; Джалилова, Д. Ш.; Tsvetkova, Ekaterina; Saveliev, S. V.

doi:10.1007/s10517-020-04807-8

Cited by 3 publications

(9 citation statements)

References 10 publications

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“…Данные, полученные при рутиной окраске гематоксилином и эозином, в целом были сходны с результатами других авторов. Однако стоит отметить, что обнаруживаемые при этой окраске субтипы клеток I типа (светлый, темный, пикнотический) являются ничем иным как артефактом аутолиза и не могут быть диагностическим маркером той или иной патологии, как было установлено нами ранее [13].…”

Section: Discussionunclassified

“…При этом по сравнению с каротидным клубочком крыс, орган которых состоит лишь из гнезд клеток [13], у человека он представлял собой скопление гнезд клеток, которые, в свою очередь, образовывали более крупные дольки, отделенные друг от друга прослойками соединительной ткани.…”

Section: Discussionunclassified

“…За исключением тирозингидроксилазы иммуногистохимические реакции в человеческом каротидном клубочке были схожими с изученными нами ранее ка- ротидными клубочками крыс [13]. Как и у крыс, все клетки I типа были положительны на bIII-тубулин, PGP9.5, синаптофизин, шванновские клетки и клетки II типа -на S100 и GFAP, нервные волокна -на bIIIтубулин, PGP9.5 и нейрофиламенты 200кД.…”

Section: Discussionunclassified

“…Как и у крыс, все клетки I типа были положительны на bIII-тубулин, PGP9.5, синаптофизин, шванновские клетки и клетки II типа -на S100 и GFAP, нервные волокна -на bIIIтубулин, PGP9.5 и нейрофиламенты 200кД. Анализ литературы показывает, что в этом плане каротидный клубочек человека сходен с каротидным клубочком не только крыс [13,14], но и мышей [15]. Тем не менее было отмечено, что количество положительных на тирозингидроксилазу клеток у человека значительно меньше.…”

Section: Discussionunclassified

“…Тем не менее было отмечено, что количество положительных на тирозингидроксилазу клеток у человека значительно меньше. Если у крыс и мышей отмечалась положительная реакция во всех клетках I типа [13][14][15], у человека ее показывали лишь единичные клетки. Причем это не является артефактом отсроченной фиксации, так как полученные нами ранее данные свидетельствуют о том, что даже после суточного аутолиза реакция на тирозингидроксилазу не изменяется [13].…”

Section: Discussionunclassified

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Immunohistochemical features of the human carotid body

Junemann

Tsvetkova

Gorokhov

et al. 2020

Clin. Exp. Morphology

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Introduction. The carotid body is a chemoreceptor organ and the initial link of the reflex regulation of car-diovascular and respiratory systems. However, molecular genetic and immunohistochemical characteristics of the human carotid body remains underinvestigated. Although there are numerous studies of the second half of the 20th century devoted to the classical light-optical histology of the human organ, the immunohis-tochemical investigations are very few. The aim of our study was to clarify immunohistochemical features of the human carotid body in comparison with those of the most commonly used laboratory animals. Materials and methods. The study was performed on 10 carotid bodies of the adult human of different ages of both sexes using immunoperoxidase labeling with antibodies to bIII-tubulin, tyrosine hydroxylase, syn-aptophysin, PGP9.5, neurofilaments 200kDa, S100, and GFAP. Results. Nerve fibers passing between the lobules, as well as entering them, were positive for bIII-tubulin, tyrosine hydroxylase, PGP9.5 and neurofilaments. Type I cells had cytoplasmic reaction for bIII-tubulin and synaptophysin as well as cytoplasmic and nuclear staining for PGP9.5. At the same time, they had weaker reaction for tyrosine hydroxylase. Type II cells were positive for GFAP and S100. Conclusion. Immunohistochemical characteristics of the human carotid body were similar to those of rats and mice. The human carotid body cells and nerve fibers showed the same distribution of PGP9.5, bIII-tubulin, synaptophysin, neurofilaments, GFAP and S100 as rat and mouse carotid body cells. However, human carotid body reaction for tyrosine hydroxylase was much lower, which may indicate a smaller amount of synthesized catecholamines compared to the carotid body in rats and mice. Keywords: human carotid body, immunohistochemistry, sympathoadrenal system, tyrosine hydroxylase

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Section: Discussionunclassified

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Immunohistochemical features of the human carotid body

Junemann

Tsvetkova

Gorokhov

et al. 2020

Clin. Exp. Morphology

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Comparative Morphological and Immunohistochemical Characteristics of the Human Fetal Organs of Zuckerkandl and Adrenal Medulla

Otlyga,

Junemann

et al. 2024

Preprint

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The adrenal medulla and organs of Zuckerkandl consist of chromaffin cells that produce, store, and secrete catecholamines. In humans, the adrenal medulla is known to function throughout postnatal life, while the organs of Zuckerkandl degenerate by 2–3 years of postnatal life. Although the history of investigation of chromaffin cells goes back more than a century, little is known about the interaction of the adrenal glands and organs of Zuckerkandl in human fetal development. In the current study, we attempted to compare these two organs using serial sectioning, routine histological staining, and immunohistochemical reactions in human embryos, prefetuses, and fetuses from 8 to 26 gestational weeks. In our study, we used antibodies for tyrosine hydroxylase, dopamine beta-hydroxylase, and phenylethanolamine N-methyltransferase, which are enzymes of catecholamine synthesis, β-III tubulin, and S100. We found two morphological cell types (large and small) in the developing ganglia, organs of Zuckerkandl, and adrenal medulla and two migration patterns of large cells and small cells from the developing paraganglia to the adrenal anlage. We determined the immunohistochemical characteristics of these migrating cells. We also determined that 12 gestational weeks was the age of the first appearance of phenylethanolamine N-methyltransferase reactivity in developing chromaffin cells, which is important data in the light of the controversial glucocorticoid theory of phenylethanolamine N-methyltransferase induction in humans.

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Immunohistochemical Characteristics of the Human Carotid Body in the Antenatal and Postnatal Periods of Development

Otlyga

Tsvetkova

Junemann

et al. 2021

IJMS

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The evolutionary and ontogenetic development of the carotid body is still understudied. Research aimed at studying the comparative morphology of the organ at different periods in the individual development of various animal species should play a crucial role in understanding the physiology of the carotid body. However, despite more than two centuries of study, the human carotid body remains poorly understood. There are many knowledge gaps in particular related to the antenatal development of this structure. The aim of our work is to study the morphological and immunohistochemical characteristics of the human carotid body in the antenatal and postnatal periods of development. We investigated the human carotid bodies from 1 embryo, 20 fetuses and 13 adults of different ages using samples obtained at autopsy. Immunohistochemistry revealed expression of βIII-tubulin and tyrosine hydroxylase in the type I cells and nerve fibers at all periods of ontogenesis; synaptophysin and PGP9.5 in the type I cells in some of the antenatal cases and all of the postnatal cases; 200 kDa neurofilaments in nerve fibers in some of the antenatal cases and all of the postnatal cases; and GFAP and S100 in the type II cells and Schwann cells in some of the antenatal cases and all of the postnatal cases. A high level of tyrosine hydroxylase in the type I cells was a distinctive feature of the antenatal carotid bodies. On the contrary, in the type I cells of adults, the expression of tyrosine hydroxylase was significantly lower. Our data suggest that the human carotid body may perform an endocrine function in the antenatal period, while in the postnatal period of development, it loses this function and becomes a chemosensory organ.

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Immunohistochemical Study of Dark and Progenitor Carotid Body Cells: Artefacts or Real Subtypes?

Cited by 3 publications

References 10 publications

Immunohistochemical features of the human carotid body

Immunohistochemical features of the human carotid body

Comparative Morphological and Immunohistochemical Characteristics of the Human Fetal Organs of Zuckerkandl and Adrenal Medulla

Immunohistochemical Characteristics of the Human Carotid Body in the Antenatal and Postnatal Periods of Development

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