Immunohistochemical Study of Lactate Dehydrogenase Isozymes in Rat Liver

Glucose-6-phosphate dehydrogenase cytochemistry using a copper ferrocyanide method and its application to rapidly frozen cells

“…The specific function of ribosome-associated G6PD is not yet fully understood. Nonetheless, a similar morphological relationship between ribosome-associated ER (i.e., rough ER) and several other dehydrogenases has been previously reported (Chida et al 1975;Watabiki 1979;Yamashita et al 1979;Tanahashi and Hori 1980). Further enzyme cytochemical and immunocytochemical studies of the subcellular distribution of dehydrogenases in a variety of cell types and under different physiological conditions should provide valuable insight into the ultrastructural localization and role of the enzymes.…”

“…This technique was first introduced to the field of dehydrogenase enzyme cytochemistry by Ogawa et al (1968), who used it to detect succinate dehydrogenase. It has since been employed to detect other dehydrogenases including sorbitol dehydrogenase (Chida et al 1975), 3β-hydroxysteorid dehydrogenase (Berchtold 1977), lactate dehydrogenase (Yamashita et al 1979), and alcohol dehydrogenase (Watabiki 1979). We have now utilized this method to demonstrate the distribution of G6PD at the electron microscopic level.…”

Ultracytochemistry of Glucose-6-Phosphate Dehydrogenase in Adrenal Gland

“…The immunohistochemical demonstration of G6PD also reports a basically similar localization on the r-ER (Tanahashi and Hori, 1980). In addition, other soluble enzymes are also known positive in the cytoplasm immediately adjacent to the r-ER ultracytochemically (Watabiki et al , 1979, Fahimi and Karnovsky, 1966, Chida et al, 1975, Baba and Sharma, 1971Yamashita et al, 1979). Applying as low a concentration of glutaraldehyde as 0.25% with 1% paraformaldehyde, it might not be easy to detect G6PD as being in close relationship with the ribosomes, but just remain to say in hyaloplasm.…”

V-1 Electron Microscopy

“…Adrenodoxin and adrenodoxin reductase have been located in the inner mitochondrial membrane of cells in the zona fasciculata and reticularis of the adrenal cortex (Izumi et al, 1979). LDH isozymes in the liver were studied by Yamashita et al (1979), and aldolase isozymes were examined in various organs . Prolactin was demonstrated in the rat hypophysis as well as in human adenoma in the cisternae of the endoplasmic reticulum, the perinuclear space and Golgi saccules (Osamura et al, 1980(Osamura et al, , 1982.…”