Immunohistochemical study of mucin expression in periampullary adenomyoma

Higashi, Michiyo; Goto, Masamichi; Saitou, Miyuki; Shimizu, Takeshi; Rousseau, Karine; Batra, Surinder K.; Yonezawa, Suguru

doi:10.1007/s00534-009-0176-5

Cited by 16 publications

(20 citation statements)

References 23 publications

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“…For MUC3 staining, the sections were treated at 100°C for 10 min in 0.01 M citrate buffer at pH 6.0 and then reduced with 0.01 M dithiothreitol in 0.1 M Tris/HCl buffer (pH 8.0) for 30 min at room temperature and alkylated with 0.025 M iodoacetamide in 0.1 M Tris/HCl buffer (pH 8.0) for 30 min [10], [15]. They were then incubated with mMUC3-1 at 4°C for 16 h and stained by the avidin-biotin complex method.…”

Section: Methodsmentioning

confidence: 99%

Pathobiological Implications of Mucin (MUC) Expression in the Outcome of Small Bowel Cancer

et al. 2014

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Mucins have been associated with survival in various cancer patients, but there have been no studies of mucins in small bowel carcinoma (SBC). In this study, we investigated the relationships between mucin expression and clinicopathologic factors in 60 SBC cases, in which expression profiles of MUC1, MUC2, MUC3, MUC4, MUC5AC, MUC6 and MUC16 in cancer and normal tissues were examined by immunohistochemistry. MUC1, MUC5AC and MUC16 expression was increased in SBC lesions compared to the normal epithelium, and expression of these mucins was related to clinicopathologic factors, as follows: MUC1 [tumor location (p = 0.019), depth (p = 0.017) and curability (p = 0.007)], MUC5AC [tumor location (p = 0.063) and lymph node metastasis (p = 0.059)], and MUC16 [venous invasion (p = 0.016) and curability (p = 0.016)]. Analysis of 58 cases with survival data revealed five factors associated with a poor prognosis: poorly-differentiated or neuroendocrine histological type (p<0.001), lymph node metastasis (p<0.001), lymphatic invasion (p = 0.026), venous invasion (p<0.001) and curative resection (p<0.001), in addition to expression of MUC1 (p = 0.042), MUC5AC (p = 0.007) and MUC16 (p<0.001). In subsequent multivariate analysis with curability as the covariate, lymph node metastasis, venous invasion, and MUC5AC and/or MUC16 expression were significantly related to the prognosis. Multivariate analysis in curative cases (n = 45) showed that SBC with MUC5AC and/or MUC16 expression had a significantly independent high hazard risk after adjusting for the effects of venous invasion (hazard ratio: 5.6, 95% confidence interval: 1.8–17). In conclusion, the study shows that a MUC5AC-positive and/or MUC16-positive status is useful as a predictor of a poor outcome in patients with SBC.

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Section: Methodsmentioning

confidence: 99%

Pathobiological Implications of Mucin (MUC) Expression in the Outcome of Small Bowel Cancer

et al. 2014

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“…For example, normal pancreatic tissue mainly expresses MUC1 and MUC6, while ampullary adenocarcinoma mainly expresses MUC1 and MUC5AC [10], along with MUC6 and MUC2 [3,11,12]. Combined detection of mucins has been used to diagnose and differentiate pancreatic ductal adenocarcinoma and ampullary adenocarcinoma [11-15]. …”

Section: Introductionmentioning

confidence: 99%

Mucins Differently Expressed in Various Ampullary Adenocarcinomas

Wang

Liang

et al. 2011

Diagn Pathol

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BackgroundWe investigated the occurrence and clinical significance of mucin expression in ampullary adenocarcinoma.MethodsWe retrospectively analyzed clinical, pathological, and survival data from 74 ampullary adenocarcinoma patients who received radical operation from January 2004 to November 2006.ResultsThe tumors were located in the lower end of the common bile duct (46%), papillary duodenum (42%), and ampullary duodenum (12%), and expressed MUC1 (72%), MUC2 (20%), MUC5AC (43%), and MUC6 (27%). Expression of MUC1 was associated with tumor differentiation (OR: 4.71, 95% CI: 1.26, 17.66, P = 0.021). Expression of MUC5AC was associated with age (OR: 1.07, 95% CI: 1.11, 1.14, P = 0.026) and less vessel invasion(OR: 0.14, 95% CI: 0.03, 0.72, P = 0.019). The survival rates were not significantly different when patients had or had no expression of MUC1, MUC2, MUC5AC, or MUC6 in tumor. Patients with tumors positive for MUC5AC in the papillary duodenum had worse survival than those with tumors negative for MUC5AC (P = 0.044).ConclusionsExpression of MUC1 was high (72%) in ampullary adenocarcinoma, while expressions of MUC2, MUC5AC, and MUC6 were lower. Mucins are useful markers to diagnose and identify ampullary adenocarcinoma, particularly in determining the degree of malignancy of ampullary adenocarcinoma.

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“…Immunohistochemical stainings were done by an immunoperoxidase method using the ABC complex as described previously [2,6,9,15] . For the retrieval of MUC4, the sections were treated in a water bath at 80 ° C for 20 min in 0.01 M citrate buffer (pH 6.0).…”

Section: Staining Proceduresmentioning

confidence: 99%

Pathobiological Implications of MUC16/CA125 Expression in Intrahepatic Cholangiocarcinoma-Mass Forming Type

Higashi¹,

Yamada²,

Yokoyama³

et al. 2012

Pathobiology

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Objectives: MUC16 carries the peptide epitope CA125, which is well known as a marker of ovarian cancer. High serum levels of MUC16 (CA125) have been reported not only in patients with ovarian cancer but also in patients with liver diseases. We evaluated the expression of MUC16 in intrahepatic cholangiocarcinoma-mass forming type (ICC-MF) tissues. Methods: We examined the expression of MUC16 by immunohistochemical analyses using the monoclonal antibody M11 in ICC-MF tissues from 63 patients. To compare the prevalence of each mucin expression by clinicopathological features, appropriate statistical analysis was performed. Results: MUC16 was detected in 48% of samples (30/63). After adjusting for the effects of other prognostic factors, multivariate survival analysis revealed that MUC16 expression is a significant independent factor of poor prognosis (p = 0.005). Conclusion: The current results indicate that MUC16 expression is a prognostic factor of poor survival in ICC-MF.

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Immunohistochemical study of mucin expression in periampullary adenomyoma

Abstract: A combined evaluation of IHC findings may be effective in the detection of AM and AMH, and also in distinguishing benign periampullary lesions, such as AM and AMH, from ampulla of Vater adenocarcinoma, thus avoiding excessive surgery.

Cited by 16 publications

References 23 publications

Pathobiological Implications of Mucin (MUC) Expression in the Outcome of Small Bowel Cancer

Pathobiological Implications of Mucin (MUC) Expression in the Outcome of Small Bowel Cancer

Mucins Differently Expressed in Various Ampullary Adenocarcinomas

Pathobiological Implications of MUC16/CA125 Expression in Intrahepatic Cholangiocarcinoma-Mass Forming Type

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