Immunohistochemical study of <scp>CD</scp>34 and podoplanin in periodontal disease

Gonçalves, Patrícia Guerra Peixe; Lourenço, Salomão Israel Monteiro; Gurgel, Bruno César de Vasconcelos

doi:10.1111/jre.12635

Cited by 5 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CD34 is a transmembrane glycoprotein grouped into the CD34 family. It is found in hematopoietic stem cells in the vascular endothelium of small vessels in a few neoplastic cell lines 19. As shown in Figure 5, the CD34 markers in the blood vessels with cytoplasmic and/or membrane labeling were observed, and the expression of IL-9 and CD34 proteins in the Model group was positive.…”

Section: Resultsmentioning

confidence: 85%

<p>Effect of Th9/IL-9 on the growth of gastric cancer in nude mice</p>

Cai¹,

Zhang²,

Zhang³

et al. 2019

OTT

View full text Add to dashboard Cite

Objective By neutralizing IL-9 in a nude mouse model, the study aimed to investigate the role of Th9/IL-9 on the growth of gastric cancer in mice. Materials and methods Male BALB/c nude mice were randomly divided into three groups: a normal control group (Control), an SGC-7901 xenografted nude mice model group (Model), and a rIL-9 treatment group (Treat). The weight of the tumors was recorded to calculate the tumor inhibition rate. Flow cytometry was used to detect the cell frequency of Th9, Th17, and Treg in peripheral blood. The IL-4, IL-9, IL-10, IL-25, VEGF, and TGF-β levels in serum were determined by ELISA. The cellular migration and invasion were investigated by transwell assay. Immunohistochemical and Western blot were used to detect the expression of IL-9, CD34, PU.1, p53, and p21 proteins in gastric cancer tissue. The mRNA expression levels of IL-9, IL-21, and PU.1 in gastric cancer tissue were determined by qRT-PCR. Result rIL-9 can significantly inhibit the growth of gastric cancer. The frequency of Th9, Th17, and Treg in peripheral blood was decreased upon treatment. The levels of IL-4, IL-9, IL-10, IL-25, VEGF, and TGF-β in serum were significantly reduced in the Treat group compared with the Model group ( P <0.05). rIL-9 can inhibit cellular migration and invasion and reduce the mRNA level of IL-9, IL-21, and PU.1. Meanwhile, in the Treat group, the expression of IL-9, CD34, and PU.1 was significantly reduced, whereas the expression of p53 and p21 was significantly increased compared with the Model group ( P <0.05). Conclusion This study suggested that Th9/IL-9 has a deleterious role in gastric cancer.

show abstract

Section: Resultsmentioning

confidence: 85%

<p>Effect of Th9/IL-9 on the growth of gastric cancer in nude mice</p>

Cai¹,

Zhang²,

Zhang³

et al. 2019

OTT

View full text Add to dashboard Cite

show abstract

“…Additionally, no correlation with mRSS could be shown, but the number of patients with SSc was very low. Immunohistochemically, neither surface antigen has been detected in either healthy or patients with P thus far [46]. This argues for the methodology chosen here for further studies and the inclusion of patients with P.…”

Section: Discussionmentioning

confidence: 90%

Investigation of the Expression of Inflammatory Markers in Oral Biofilm Samples in Patients with Systemic Scleroderma and the Association with Clinical Periodontal Parameters—A Preliminary Study

Buchbender

Lugenbühl

Fehlhofer

et al. 2021

Life

View full text Add to dashboard Cite

Background: Systemic scleroderma (SSc) has multiple orofacial effects. The aim of this study was to analyze the expression of inflammatory mediators in biofilm samples. It was hypothesized that different expression levels and clinical associations might be drawn. Methods: A total of 39 biofilm samples from group 1 = SSc and group 2 = healthy control were examined for the expression levels of interleukin (IL)-2,-6, and -10; matrix metalloprotease (MMP)-9; and surface antigens CD90 and CD34 by quantitative real-time PCR and clinical parameters. Relative quantitative (RQ) gene expression was determined using the ∆∆CT method. Results: The mean bleeding on probing values (p = 0.006), clinical attachment loss (CAL) (p = 0.009), gingival recession (p = 0.020), limited mouth opening (p = 0.001) and cervical tooth defects (p = 0.011) were significantly higher in group 1. RQ expressions of IL-2 and CD34 were significantly lower, IL-6, MMP-9, and CD90 were significantly higher. There was a significant positive correlation of IL-6/MMP-9 and negative correlation of mouth opening/CAL and IL-6/CAL. Conclusion: Different expression levels of IL-2, IL-6, MMP-9, CD34 and CD90 were detected in biofilm samples from patients with SSc compared to control. An immunological correlation to the clinical parameters of mouth opening and CAL was shown; thus, we conclude that SSc might have an impact on periodontal tissues.

show abstract

“…In a study on sheep injected with radioactive albumin in gingiva, as much as 64.7% of the injected protein was recovered in the prescapular and cervical lymph vessels 7.5 h after injection (15). The gingiva is well supplied with lymphatic vessels (7,16,17), and when lymph is formed, muscle movements and soft tissue deformation may promote a rapid transport of fluid via the lymphatic system. This is in contrast to the bone‐embedded interstitial tissue in the mandibular canal, and may explain why we observed a much slower washout rate from this compartment.…”

Section: Discussionmentioning

confidence: 99%

Interstitial fluid drainage from rat apical area takes place via vessels in the mandibular canal

Virtej

Tenstad

Wiig

et al. 2020

European J Oral Sciences

View full text Add to dashboard Cite

Interstitial fluid drainage from rat apical area takes place via vessels in the mandibular canal Virtej A, Tenstad O, Wiig H, Berggreen E. Interstitial fluid drainage from rat apical area takes place via vessels in the mandibular canal. Eur J Oral Sci 2020; 128: 190-195.We sought to investigate the transport route for protein-rich fluid from the apical area towards the draining lymph nodes. The first mandibular molar root canals in 24 female Wistar rats were instrumented and filled with radioactive-labelled human serum albumin. The rats were sacrificed at different intervals beginning after 10 min (time 0) and continuing up to 72 h. Three jaw segments, gingiva around the first molar, blood samples, submandibular and cervical lymph nodes were collected and analyzed for radioactivity. The starting volume of tracer (control) for all experiments was calculated from measurements at time 0. At time 0, radioactivity was only detected in the jaw segments. Within lymph nodes and serum, the tracer was found after 4 h, with the highest amount recorded in serum up to 24 h. Lymphatics were found within the mandibular canal along blood vessels and nerves and exiting via foramen mandibularis, after immunohistochemical staining in four untreated rats. Our results show tracer distribution from the apical area towards the mandibular canal in a posterior direction. The tracer washout rate was low, and the fluid was mainly absorbed into blood vessels. The lymphatics in the mandibular canal may be more important for immune cell transport than for fluid drainage.

show abstract

Immunohistochemical study of CD34 and podoplanin in periodontal disease

Cited by 5 publications

References 20 publications

<p>Effect of Th9/IL-9 on the growth of gastric cancer in nude mice</p>

<p>Effect of Th9/IL-9 on the growth of gastric cancer in nude mice</p>

Investigation of the Expression of Inflammatory Markers in Oral Biofilm Samples in Patients with Systemic Scleroderma and the Association with Clinical Periodontal Parameters—A Preliminary Study

Interstitial fluid drainage from rat apical area takes place via vessels in the mandibular canal

Contact Info

Product

Resources

About