Immunohistochemical study of secretory IGA in the human male reproductive tract

Sirigu, P.; Perra, Maria Teresa; Turno, F.

doi:10.1111/j.1439-0272.1995.tb01368.x

Cited by 11 publications

(5 citation statements)

References 19 publications

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“…The presence of PIGR was previously reported in the semen of bulls (Rego et al, 2014) and cats (Mogielnicka-Brzozowska et al, 2020). In humans, PIGR expression was observed in the prostate (Sirigu et al, 1995) which agrees with our results as the prostate is the only accessory sex gland in dolphins.…”

Section: Proteins Identified In the Seminal Plasma Of Bottlenose Dolphinsupporting

confidence: 93%

Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)

Fuentes-Albero

González-Brusi

Cots

et al. 2021

Front. Cell Dev. Biol.

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Proteins play an important role in many reproductive functions such as sperm maturation, sperm transit in the female genital tract or sperm-oocyte interaction. However, in general, little information concerning reproductive features is available in the case of aquatic animals. The present study aims to characterize the proteome of both spermatozoa and seminal plasma of bottlenose dolphins (Tursiops truncatus) as a model organism for cetaceans. Ejaculate samples were obtained from two trained dolphins housed in an aquarium. Spermatozoa and seminal plasma were analyzed by means of proteomic analyses using an LC-MS/MS, and a list with the gene symbols corresponding to each protein was submitted to the DAVID database. Of the 419 proteins identified in spermatozoa and 303 in seminal plasma, 111 proteins were shared by both. Furthermore, 70 proteins were identified as involved in reproductive processes, 39 in spermatozoa, and 31 in seminal plasma. The five most abundant proteins were also identified in these samples: AKAP3, ODF2, TUBB, GSTM3, ROPN1 for spermatozoa and CST11, LTF, ALB, HSP90B1, PIGR for seminal plasma. In conclusion, this study provides the first characterization of the proteome in cetacean sperm and seminal plasma, opening the way to future research into new biomarkers, the analysis of conservation capacity or possible additional applications in the field of assisted reproductive technologies.

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Section: Proteins Identified In the Seminal Plasma Of Bottlenose Dolphinsupporting

confidence: 93%

Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)

Fuentes-Albero

González-Brusi

Cots

et al. 2021

Front. Cell Dev. Biol.

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“…22,24,31,32 In fact, the prostate is considered the major site of IgA access into the MRT and this expression can be further induced in the upper MRT if ascending infections occur, 31 strongly supporting a role for the prostate and the pIgR in protection against ascending infections of the MRT. To examine the effect of neutralizing IgA antibodies on infection, the numbers of epithelial cells with chlamydial inclusions were determined by immunohistochemistry.…”

Section: Discussionmentioning

confidence: 98%

“…Expression of the secretory component of the pIgR is prominent in the urethra and prostate. 22,24,31,32 In fact, the prostate is considered the major site of IgA access into the MRT and this expression can be further induced in the upper MRT if ascending infections occur, 31 strongly supporting a role for the prostate and the pIgR in protection against ascending infections of the MRT. The presented findings support the literature, with infection of the epithelial cells greatest in the urethra, the site of initial infection, while the effect of pIgR-transytosed IgA was greatest in the prostate, and testicular infection was low in both groups.…”

Section: Discussionmentioning

confidence: 98%

“…[22][23][24] As pIgR-mediated transport is the main pathway for hepatic and intestinal transport of IgA in mice, 25 we examined whether pIgR contributes to transport of soluble IgA into the male reproductive tract. The presence of the pIgR has been detected in the epithelial cells of the prostate and penile urethral glands suggesting a role for this transport pathway in the MRT.…”

Section: Introductionmentioning

confidence: 99%

“…The presence of the pIgR has been detected in the epithelial cells of the prostate and penile urethral glands suggesting a role for this transport pathway in the MRT. [22][23][24] As pIgR-mediated transport is the main pathway for hepatic and intestinal transport of IgA in mice, 25 we examined whether pIgR contributes to transport of soluble IgA into the male reproductive tract. To this end, we immunized both C57BL ⁄ 6 wild-type mice and pIgR ) ⁄ ) mice, to determine if pIgR-mediated transcytosis of IgA in the MRT is required for protection against chlamydial infection.…”

Section: Introductionmentioning

confidence: 99%

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ORIGINAL ARTICLE: Poly‐Immunoglobulin Receptor‐Mediated Transport of IgA into the Male Genital Tract is Important for Clearance of Chlamydia muridarum Infection

Cunningham¹,

Carey²,

Finnie³

et al. 2008

American J Rep Immunol

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Distribution of leukocytes in the epithelium and interstitium of four regions of the Lewis rat epididymis

et al. 1997

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Background Leukocytes expressing different surface markers were studied in four regions of the epididymis of Lewis rats. Cells resembling lymphocytes or monocytes had been described in the epididymis, but previous studies differed as to their nature and immunologic significance. Methods: Frozen sections were immunocytochemically stained with monoclonal antibodies W3/25, OX‐8, OX‐42, and RLN‐9D3, which are directed toward markers on CD4+ cells, CD8+ cells, macrophages, and B lymphocytes, respectively. The concentration of stained cells in the epithelium and interstitial tissue of the initial segment, caput, proximal cauda, and distal cauda regions was determined by a procedure based on the optical disector method. Results: CD4+ leukocytes were present in greater concentration than CD8+ cells or macrophages in both the epithelium and interstitial tissue of all four regions. In the epithelium, the concentration of CD8+ leukocytes was greater than that of macrophages in the initial segment, caput, and distal cauda. In the interstitium, however, the concentration of macrophages exceeded that of CD8+ cells in both parts of the cauda. Macrophages and T lymphocytes were generally present in greater concentrations in the interstitium than in the epithelium, especially in the more proximal parts of the epididymis. In contrast to T cells, B lymphocytes were not detected in the interstitium or epithelium of any of part of the epididymis, despite prominent staining of B cells in other locations. Conclusions: The epididymal epithelium of the Lewis rat contains many T lymphocytes, which may correspond to ‘halo’ cells. CD4+ leukocytes predominate in all regions of the epididymis. The interstitium may function as a reservoir of leukocytes for the epithelial compartment. The epididymis is not normally a site for local immunoglobulin synthesis. Anat. Rec. 248:380‐390, 1997. © 1997 Wiley‐Liss, Inc.

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Immunohistochemical study of secretory IGA in the human male reproductive tract

Cited by 11 publications

References 19 publications

Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)

Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)

ORIGINAL ARTICLE: Poly‐Immunoglobulin Receptor‐Mediated Transport of IgA into the Male Genital Tract is Important for Clearance of Chlamydia muridarum Infection

Distribution of leukocytes in the epithelium and interstitium of four regions of the Lewis rat epididymis

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