Immunohistochemical study of the expression of human chorionic gonadotropin-? in oral squamous cell carcinoma

Bhalang, Kanokporn; Kafrawy, Abdel H.; Miles, Dale A.

doi:10.1002/(sici)1097-0142(19990215)85:4<757::aid-cncr1>3.0.co;2-t

Cited by 37 publications

(27 citation statements)

References 22 publications

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“…The average follow-up time was 42.9 months. Immunhistochemistry was performed using the avidinbiotin-peroxidase complex technique (ABC-technique) [30,47]. Paraffin sections measuring 4 μm in thickness of the excised tumours were placed on positively charged glass sides.…”

Section: Patients and Specimensmentioning

confidence: 99%

Impact of podoplanin expression in oral squamous cell carcinoma: clinical and histopathologic correlations

et al. 2010

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Cervical lymph node metastases reduce the overall survival of patients with oral squamous cell carcinoma (OSCC) and require a neck dissection. However, elective management of a clinical N0 neck remains a controversial topic, as there are no reliable factors available predicting cervical lymph node metastases. Recent studies suggest an impact of podoplanin expression on metastatic spread to the cervical lymph nodes. Our aim was to investigate the influence of podoplanin expression on prognosis and metastatic lymphatic spread. In our retrospective study, podoplanin expression was examined in a set of 80 patients with OSCC by immunohistochemistry. We analysed associations between the level of podoplanin expression and various clinicopathologic parameters. In 67 patients (84%), podoplanin was expressed on the tumour cells. Nineteen patients (24%) showed high levels of expression. The 5-year overall survival (31%) for patients with high levels of podoplanin expression was significantly lower (p < 0.001) than for patients with low and moderate expression of podoplanin (93% and 65%, respectively). There was an association between podoplanin expression and the frequency of cervical lymph node metastases. Cervical lymph node metastases were found in 79% of the patients with high podoplanin expression, while patients with weak podoplanin expression had metastases in only 22% (p < 0.001). None of the 13 patients without podoplanin expression had cervical lymph node metastases. We concluded that podoplanin is expressed frequently in OSCC and that podoplanin expression correlates with cervical lymph node metastases and clinical outcome.

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Section: Patients and Specimensmentioning

confidence: 99%

Impact of podoplanin expression in oral squamous cell carcinoma: clinical and histopathologic correlations

et al. 2010

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“…4 Intact hCG appears to be the main form of hCG immunoreactivity (hCG-IR) during growth and local invasion of a tumor, whereas expression of the free ␤ subunit (hCG␤) correlates more closely with a metastatic phenotype. 4 hCG-IR has been detected in a wide variety of non-trophoblastic neoplasms in both tumor cells [5][6][7] and serum. 9 -14 In colorectal cancer, the reported rate of elevated serum hCG is between 2% and 41% 2,3,12,14 -17 and the rate of immunohistochemical tissue expression is between 17% and 52 %.…”

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confidence: 99%

Tissue expression of human chorionic gonadotropin ? predicts outcome in colorectal cancer: A comparison with serum expression

et al. 2001

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“…14 Of interest for this study is the observation that normal tissue, for example oral mucosa, does not express hCG. 15 Past experiments have also shown that detecting hCG in serous fluids or urine using laboratory methods indicates the presence of malignancy in these specimens. 7,8,16 Prior reports indicate that 31-42% of malignant effusions will have elevated hCG levels as compared to benign effusions using laboratory methods.…”

Section: Discussionmentioning

confidence: 99%

The β subunit of human chorionic gonadotropin lacks specificity for malignant cells in serous effusions

Zimmerman

Fogt

2004

Modern Pathology

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The cytologic diagnosis of malignancy is frequently straightforward. For difficult cases, multiple immunostains and immunostain panels have been investigated without consensus. b-human chorionic gonadotropin (hCG) has been reportedly expressed in malignancies, but not in normal tissue. HCG also has been reported as a specific marker of metastases in serous fluids when detected with laboratory assays. We investigated the clinical utility of hCG in this cytologic setting. A total of 97 cases of benign and malignant effusions were studied. Each case was immunostained with monoclonal hCG using the avidin-biotin technique and diaminobenzidine as a chromogen. Additionally, a mucicarmine stain was performed on most cases. Cases were evaluated for hCG expression and mucin in a blinded fashion. After the cases were reviewed, the diagnoses were unblinded and staining patterns were evaluated. Of the 47 benign cases studied, 23 (49%) exhibited immunoreactivity to hCG in at least 5% of mesothelial cells present. In contrast, 28 of 44 (64%) adenocarcionomas exhibited a similar degree of immunostaining. In all, 21 (48%) of the adenocarcinomas were also positive for mucin; five of these mucin-positive cases were negative for hCG. The combination of mucin and hCG detected 33 of 44 (75%) adenocarcinomas. We conclude that hCG lacks the specificity for malignant cells to be of clinical use in effusion cytology.

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Immunohistochemical study of the expression of human chorionic gonadotropin-? in oral squamous cell carcinoma

Cited by 37 publications

References 22 publications

Impact of podoplanin expression in oral squamous cell carcinoma: clinical and histopathologic correlations

Impact of podoplanin expression in oral squamous cell carcinoma: clinical and histopathologic correlations

Tissue expression of human chorionic gonadotropin ? predicts outcome in colorectal cancer: A comparison with serum expression

The β subunit of human chorionic gonadotropin lacks specificity for malignant cells in serous effusions

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