Immunohistochemically detected ontogeny of prolactin and growth hormone cells in the African catfish Clarias gariepinus

Volckaert, Filip; Mugoyo, James; Lescroart, Olivier; Grisez, L.; Ollevier, Frans

doi:10.1016/s0305-0491(99)00017-6

Cited by 13 publications

(6 citation statements)

References 38 publications

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“…The defined localisation of cells immunoreactive for PRL in the RPD and for GH around the neurohypophyseal processes in the PPD as found in the present study has been observed for various teleosts (Yan and Thomas 1991;Power 1992;García-Hernández et al 1996;Weltzien et al 2003b;Volckaert et al 1999;Laiz-Carrión et al 2003), including tilapia (Nishioka et al 1993;Nagahama et al 1981;Mousa and Mousa 1999a,b).…”

Section: Discussionsupporting

confidence: 85%

A systematic immunohistochemical survey of the distribution patterns of GH, prolactin, somatolactin, β–TSH, β–FSH, β–LH, ACTH, and α–MSH in the adenohypophysis of Oreochromis niloticus, the Nile tilapia

et al. 2006

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Fish pituitary plays a central role in the control of growth, development, reproduction and adaptation to the environment. Several types of hormone-secreting adenohypophyseal cells have been characterised and localised in diverse teleost species. The results suggest a similar distribution pattern among the species investigated. However, most studies deal with a single hormone or hormone family. Thus, we studied adjacent sections of the pituitary of Oreochromis niloticus, the tilapia, by conventional staining and immunohistochemistry with specific antisera directed against growth hormone (GH), prolactin (PRL), somatolactin (SL), thyrotropin (beta-TSH), follicle-stimulating hormone (beta-FSH), luteinising hormone (beta-LH), adrenocorticotropic hormone (ACTH) and melanocyte-stimulating hormone (alpha-MSH). The pituitary was characterised by a close interdigitating neighbourhood of neurohypophysis (PN) and adenohypophysis. PRL-immunoreactive and ACTH-immunoreactive cells were detected in the rostral pars distalis. GH-immunoreactive cells were present in the proximal pars distalis (PPD). A small region of the PPD contained beta-TSH-immunoreactive cells, and beta-LH-immunoreactive cells covered approximately the remaining parts. Centrally, beta-FSH-immunoreactive cells were detected in the vicinity of the GH-containing cells. Some of these cells also displayed beta-LH immunoreactivity. The pars intermedia was characterised by branches of the PN surrounded by SL-containing and alpha-MSH-immunoreactive cells. The ACTH and alpha-MSH antisera were observed to cross-react with the respective antigens. This cross-reactivity was abolished by pre-absorption. We present a complete map of the distinct localisation sites for the classical pituitary hormones, thereby providing a solid basis for future research on teleost pituitary.

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Section: Discussionsupporting

confidence: 85%

A systematic immunohistochemical survey of the distribution patterns of GH, prolactin, somatolactin, β–TSH, β–FSH, β–LH, ACTH, and α–MSH in the adenohypophysis of Oreochromis niloticus, the Nile tilapia

et al. 2006

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“…Several cytochemical investigations focused on the identification and localization of ADH cells types in teleost species, including representative species of the following orders: Cyprinodontiformes (Batten, ), Osteoglossiformes (Borella et al, ), Perciformes (García‐Ayala et al, ; Villaplana et al, ; Pandolfi et al, ); Clupeiformes (Laiz‐Carrión et al, ), Salmoniformes (Naito et al, ), Siluriformes (Volckaert et al, ), Cypriniformes (Chapman et al, ), Osmeriformes (Saga et al, ), Pleuronectiformes (Einarsdóttir et al, ), and Atheriniformes (Vissio et al, ). Among the reophilic Neotropical species, only S. hilarii (Honji et al, ), S. brasiliensis (Jesus et al, ) and Piaractus mesopotamicus (Borella et al, ), all Characiformes, were previously analyzed, adding for the first time to the list the tropical catfish S. parahybae in this study.…”

Section: Discussionmentioning

confidence: 99%

Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity

Honji

Caneppele

Pandolfi

et al. 2015

The Anatomical Record

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The aim of this study was to identify and characterize pituitary cells of Steindachneridion parahybae females in captivity, highlighting the possible relationship with reproductive disorders at this level, since this species shows oocyte final maturation, ovulation and spawning dysfunction in captivity. The localization and distribution of growth hormone (GH), prolactin (PRL), somatolactin (SL), b-luteinizing hormone (b-LH), and b-follicle stimulating hormone (b-FSH) immunoreactive (-ir) cells in the adenohypophysis was studied by immunohistochemical and Western blot methods. In addition, cellular morphometric analyses and semi-quantification of ir-cells optical density (OD) during the annual reproductive cycle and after artificial induced spawning (AIS) were performed. Results showed that the distribution and general localization of pituitary cell types were similar to that of other teleost species. However, the morphometrical study of adenohypophysial cells showed differences along the reproductive cycle and following AIS. In general, females at the vitellogenic stage presented greater OD values for GH, PRL and SL than at other maturation stages (previtellogenic and regression stages), probably indicating an increased cellular activity during this stage. Conversely, b-LH OD did not vary during the annual reproductive cycle. After AIS, b-LH, SL and GH ir-cells showed an increase in OD values suggesting a possible involvement on oocyte final maturation, ovulation and spawning or a feedback control on the brain-pituitary-gonads axis. Reproductive dysfunction in S. parahybae females in captivity may be due to alteration of the synthesis pathways of b-LH. In addition, GH family of hormones could modulate associated mechanisms that influence the reproductive status in this species. Anat

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“…The early and almost simultaneous appearance of ir‐GH, ir‐PRL and ir‐SL cells suggests a common origin from a primordial stem cell, as hypothesized in the sea bream Sparus aurata (L. 1758) (Perciformes) (Villaplana et al ., ). In freshwater and brackish water teleosts, the ir‐GH cells appeared before hatching (Naito et al ., ; Saga et al ., , ) or around or immediately after hatching, for example at 1 dah in the African catfish Clarias gariepinus (Burchell 1822) (Siluriformes) (Volckaert et al ., ), at 1·5 dah in S. brasiliensis (de Jesus et al ., ) and at 2 dah in D. rerio (Herzog et al ., ). In marine teleosts the ir‐GH cells were detected at hatching or upon yolk absorption (Cambré et al ., ; Power & Canario, ; Tanaka et al ., ; Laiz‐Carrión et al ., ).…”

Section: Discussionmentioning

confidence: 99%

“…Only cells showing an entire nucleus on the slide were measured. The ratio of cell area to nucleus area was used as a measure of cell activity (Volckaert et al, 1999).…”

Section: Analysis Of Pituitary Cell Area and Nucleus Areamentioning

confidence: 99%

Immunocytochemical identification and ontogeny of adenohypophyseal cells in a cave fish, Phreatichthys andruzzii (Cypriniformes: Cyprinidae)

Grandi

Pezzi

Marchetti

et al. 2017

Journal of Fish Biology

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The morphogenesis of the pituitary gland and the chronological appearance of adenohypophyseal cells were investigated for the first time in the Somalian cave fish Phreatichthys andruzzii by immunocytochemistry. The adult adenohypophysis contained: a rostral pars distalis, with prolactin (PRL) cells arranged in follicles and adrenocorticotropic (ACTH) cells, a proximal pars distalis with somatotropic (GH), β-thyrotropic (TSH), β-gonadotropic type I (FSH) and type II (LH) cells and a pars intermedia with α-somatolactin (SL), α-melanotropic (MSH) and β-endorphin (END) cells. All regions were deeply penetrated by neurohypophyseal branches. At hatching (24 h post-fertilization) the pituitary was an oval cell mass, close to the ventral margin of diencephalon. The first immunoreactive cells appeared as follows: PRL at 0·5 days after hatching (dah), GH and SL at 1·5 dah, END at 2 dah, TSH, ACTH and MSH at 2·5 dah, FSH at 28 dah and LH at 90 dah. The neurohypophysis appeared at 5 dah and branched extensively inside the adenohypophysis at 130 dah, but there was no boundary between rostral pars distalis and proximal pars distalis at this stage. The potential indices of prolactin and growth hormone production increased until 28 and 60 dah, respectively. The potential index of growth hormone production correlated positively with total length. Activity of PRL and GH cells, measured as ratio of cell area to nucleus area, was significantly higher in juveniles than in larvae.

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Immunohistochemically detected ontogeny of prolactin and growth hormone cells in the African catfish Clarias gariepinus

Cited by 13 publications

References 38 publications

A systematic immunohistochemical survey of the distribution patterns of GH, prolactin, somatolactin, β–TSH, β–FSH, β–LH, ACTH, and α–MSH in the adenohypophysis of Oreochromis niloticus, the Nile tilapia

A systematic immunohistochemical survey of the distribution patterns of GH, prolactin, somatolactin, β–TSH, β–FSH, β–LH, ACTH, and α–MSH in the adenohypophysis of Oreochromis niloticus, the Nile tilapia

Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity

Immunocytochemical identification and ontogeny of adenohypophyseal cells in a cave fish, Phreatichthys andruzzii (Cypriniformes: Cyprinidae)

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