2014
DOI: 10.5858/arpa.2012-0762-oa
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Immunohistochemistry, Fluorescence in Situ Hybridization, and Reverse Transcription–Polymerase Chain Reaction for the Detection of Anaplastic Lymphoma Kinase Gene Rearrangements in Patients With Non–Small Cell Lung Cancer: Potential Advantages and Methodologic Pitfalls

Abstract: Context.-Echinoderm microtubule-associated proteinlike 4 gene (EML4) and anaplastic lymphoma kinase gene (ALK) fusion was shown to be the driver of tumorigenesis in approximately 3% to 5% of patients with non-small cell lung cancer (NSCLC) and is associated with response to inhibition with crizotinib. However, no complete agreement regarding the best diagnostic test for identification of ALK rearrangements has been achieved yet.Objective.-To investigate the concordance, sensitivity, and specificity of immunohi… Show more

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Cited by 31 publications
(24 citation statements)
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“…The same guideline suggests ALK IHC as an alternative method for the screening of ALK rearrangement status. A correlation between ALK IHC and ALK FISH results has been studied, and showed a good concordance between the two methods . ALK IHC might also detect cases in which mechanisms other than gene rearrangement cause ALK overexpression, as has been shown in other tumour types .…”
Section: Introductionmentioning
confidence: 69%
See 1 more Smart Citation
“…The same guideline suggests ALK IHC as an alternative method for the screening of ALK rearrangement status. A correlation between ALK IHC and ALK FISH results has been studied, and showed a good concordance between the two methods . ALK IHC might also detect cases in which mechanisms other than gene rearrangement cause ALK overexpression, as has been shown in other tumour types .…”
Section: Introductionmentioning
confidence: 69%
“…Staining was interpreted as positive if tumour cells showed a moderate or strong multifocal or diffuse expression. All positive cases showed a granular cytoplasmic pattern …”
Section: Methodsmentioning
confidence: 99%
“…Известным фактом является высокая частота встречаемости перестроек при аденокарцино-ме с перстневидными клетками [11]. В группе больных с транслокациями, выявленной в на-шем исследовании, только в 3 случаях из 12 об-наруживалась эта форма аденокарциномы, хотя ранее нами также отмечалась подобная законо-мерность [12]. Возможно, определенное влия-ние на редкую встречаемость аденокарциномы с перстневидными клетками в нашей выборке оказал тот факт, что почти в половине случаев для анализа предоставлялся материал из мета-статических очагов, где опухоль может иметь несколько другую морфологическую структуру.…”
Section: Discussionunclassified
“…In the last few years, several studies have performed ALK IHC on histological specimens with different monoclonal anti‐ALK antibodies such as D5F3, 5A4, and D9E4 . Similarly to histological samples, ICC with the clone ALK‐1 monoclonal antibody on cytological specimens showed low specificity and sensitivity .…”
Section: Alternative Assays For Detecting the Alk Status In Cytologymentioning
confidence: 99%
“…In the last few years, several studies have performed ALK IHC on histological specimens with different monoclonal anti-ALK antibodies such as D5F3, 5A4, and D9E4. [72][73][74] Similarly to histological samples, ICC with the clone ALK-1 monoclonal antibody on cytological specimens showed low specificity and sensitivity. 52,75,76 IHC with the anti-ALK antibody clone 5A4 showed a sensitivity of 95% and a specificity of 100% for NSCLC resection specimens and bronchial biopsies.…”
Section: Alternative Assays For Detecting the Alk Status In Cytologymentioning
confidence: 99%