Immunohistochemistry Using Polyester Wax

Localization of lily symptomless virus and cucumber mosaic virus in anther- and filament-derived calluses and effect of callus culture duration on virus-free bulblet production in Lilium × ‘Enchantment’

“…Enzyme-immunohistochemistry with freeze sectioning has been used to detect viruses such as CMV in situ (Ishimoto et al 1990). On the other hand, the polyester wax sectioning technique offers an opportunity for immunohistochemistry without using cryostat sectioning even if the antigen is sensitive to temperatures above 40°C (Kent 1999). The present study showed that polyester wax sectioning made it possible to detect LSV and CMV in situ by an enzyme-immunohistochemical technique.…”