Immunohistologic detection of immunoglobulins in malignant lymphomas and its value in histopathologic diagnosis

Radaszkiewicz, Thaddäus; Denk, Helmut

doi:10.1007/bf01257880

Cited by 15 publications

(5 citation statements)

References 46 publications

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“…For the demonstration of lymphoplasmacytic proliferations on routine formalin‐fixed, paraffin‐embedded tissue, immunoperoxidase techniques 14–18 and immunofluorescence techniques 19–21 without or with trypsin digestion 22 have been used.…”

Section: Commentsmentioning

confidence: 99%

Immunoenzymatic Typing of Lymphoplasmacytoid Skin Infiltrates

Burg

Kerl

Kaudewitz

et al. 1984

The Journal of Dermatologic Surgery and Oncology

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Monoclonal immunoglobulin-producing lymphomas (immunocytomas, plasmacytomas, and immunoblastomas) constitute 15 to 20% of all cutaneous lymphomas. Their differentiation from polymorphous lymphoplasmacytoid inflammatory or pseudolymphomatous infiltrates may be difficult if sections are stained for a single light chain only. It was the aim of the study to elucidate the ratio of the kappa- to lambda-positive cells in 10 lymphoproliferative, 5 pseudolymphomatous, and 42 inflammatory lymphoplasmacytoid cutaneous infiltrates and to characterize them by the type of Ig (alpha, gamma, mu, kappa, or lambda) synthesized intracellularly. An indirect immunoenzymatic double-labeling method (alkaline phosphatase and peroxidase) was used for the simultaneous demonstration of kappa and lambda light chains and alpha, gamma, and mu heavy chains in paraffin sections. Ig-producing lymphomas of the skin show patchy monoclonal proliferations of cells synthesizing kappa IgM in almost 50% of the cases (5 of 10). Monoclonality is claimed if the ratio of lambda- to kappa-positive cells is at least 1:10, or vice versa. In polyclonal inflammatory and pseudolymphomatous infiltrates, the lambda/kappa ratio never exceeds 1:5. The most common cell type found in these reactive infiltrates produces gamma heavy chain; some produce alpha chain, but only a few produce mu chain. In high-grade malignant lymphomas, the tumor cells may lose their capacity for Ig production.

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Section: Commentsmentioning

confidence: 99%

Immunoenzymatic Typing of Lymphoplasmacytoid Skin Infiltrates

Burg

Kerl

Kaudewitz

et al. 1984

The Journal of Dermatologic Surgery and Oncology

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“…Lysozyme (muramidase) may be tested for and if positive, suggests a true histiocytic malignancy [47] or hairy cell leukemia, in some cases [57], If lysozyme is negative, monoclonal immunoglobulin may be tested for and if positive, non-Hodgkin's lymphoma, Waldenstrom's macroglobinemia, or. rarely, myeloma can be diagnosed [58,59], If, however, the immunoglobulins show polyclonality. a terminal deoxynucléo tidyl transferase (TDT) procedure may be used which, if positive, would indicate a T cell lymphoma with epidermotropism'.…”

Section: What Kinds Of Controls Are Needed?mentioning

confidence: 99%

Pitfalls and Caveats in the Methodology for Immunoperoxidase Staining in Surgical Pathologic Diagnosis

Lewis¹,

Johnson²,

Cruse³

1983

Pathol Immunopathol Res

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“…Der Nachweis von monoklonalem IZ-Ig in 2 von 10 Fällen scheint daraufhin zuweisen, dass Keimzentrumstumorenwenn zwar auch nur selten -sekretorisches Immunglobulin produzieren können. Dies lässt auch das honigwabenartige Immun fluoreszenzmuster, welches auf die Anwe senheit von IgG Kappa in manchen Tumo ren zurückzuführen ist, erklären [14,15].…”

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“…Nur in 3 der untersuchten 15 Fälle konnte mono klonales IZ-Ig nachgewiesen werden. Immunhistochemische Untersuchungen von Lymphknoten zeigten ähnliche Ergebnisse [14,15], Die in Tumorgewebshomogenaten fallweise gefundene Immunglobulinvermeh rung steht ebenfalls damit im Einklang [16].…”

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“…Die histologischen Typen der lymphoblastischen Lymphome zeigen verschiedene Zellproliferationen mit unterschiedlichem Immunglobulingehalt [7], Der Nachweis von monoklonalem IgG Kappa in einem einzigen Fall (von 12 Fällen) bei sonstigem Überwiegen von IZ-Ig-negativen Tumorzell infiltraten stimmt mit den aus Lymphkno tenbiopsien gewonnenen Ergebnissen ande rer Autoren überein [15].…”

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Immunhistochemische Untersuchungen an malignen Lymphomen und Pseudolymphomen der Haut

1983

Dermatology

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Biopsy material of 172 patients with malignant cutaneous lymphomas and pseudolymphomas was examined using the immunoperoxidase technique. It can be stated that this technique appears to be a valuable aid for the classification and differential diagnosis of cutaneous lymphoproliferative and histiocytic disorders. In addition, the method provides a better understanding of the etiopathology of certain entities.

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Immunohistologic detection of immunoglobulins in malignant lymphomas and its value in histopathologic diagnosis

Cited by 15 publications

References 46 publications

Immunoenzymatic Typing of Lymphoplasmacytoid Skin Infiltrates

Immunoenzymatic Typing of Lymphoplasmacytoid Skin Infiltrates

Pitfalls and Caveats in the Methodology for Immunoperoxidase Staining in Surgical Pathologic Diagnosis

Immunhistochemische Untersuchungen an malignen Lymphomen und Pseudolymphomen der Haut

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