Immunohistologic Evaluation of Putatively Mutant p53 Protein in Cutaneous Melanocytic Neoplasms

Kaleem, Zahid; Lind, Anne C.; Humphrey, Peter A.; Swanson, Paul E.; Sueper, Robert H.; Ritter, Jon H.; Wick, Mark R.

doi:10.1177/106689699800600203

Cited by 5 publications

(3 citation statements)

References 50 publications

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“…In contrast, aberrant p53 protein isoforms are observed infrequently in radial growth phase (nonmetastasizing) melanomas. [24][25][26][27] Our observations support this construct. The mean level of mutant p53-positive tumor cells in primary melanomas was 9% compared with 54% in metastatic intracutaneous lesions.…”

Section: Discussionsupporting

confidence: 88%

Primary Melanoma of the Skin and Cutaneous Melanomatous Metastases

et al. 2004

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Through careful clinicopathologic correlation, we identified 37 metastatic melanomas in the skin, all of which had intraepidermal components. These were compared with 43 microscopically similar primary melanomas with a predetermined panel of immunostains in general use in surgical pathology, including bcl-2 protein, mutant p53 protein, Ki-67 (MIB-1), proliferating cell nuclear antigen (PCNA), alpha-isoform actin, and CD117 (c-kit protein). There was no significant difference in bcl-2 or alpha-isoform actin staining patterns of primary vs secondary cutaneous melanomas. The expression of Ki-67 generally was higher in metastatic melanomas than in primary lesions, and the same was true of mutant p53 protein labeling; however, some overlap was observed. CD117 staining was retained in 65% of metastatic melanomas (24/37) when they originated from ocular primary tumors; nevertheless, that marker was lost in virtually all of the other metastatic melanocytic neoplasms, whereas primary melanomas demonstrated consistent reactivity for c-kit protein. Although they are not definitive, these trends in immunoreactivity could facilitate the process of distinguishing the multiple primary melanoma syndrome from melanomatous metastases to the skin. That undertaking is best approached with circumspection, because clinicopathologic discriminators for this diagnostic separation are still imperfect.

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Section: Discussionsupporting

confidence: 88%

Primary Melanoma of the Skin and Cutaneous Melanomatous Metastases

et al. 2004

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“…As these stains were variably positive in our study, they are only helpful in some cases. A large proportion of desmoplastic melanomas stain positively for p53 41,42 . Of the two cases in our series stained for p53, both were negative, suggesting that p53 may emerge as a helpful distinguishing marker.…”

Section: Discussionmentioning

confidence: 57%

“…A large proportion of desmoplastic melanomas stain positively for p53. 41,42 Of the two cases in our series stained for p53, both were negative, suggesting that p53 may emerge as a helpful distinguishing marker. Staining for BRAFV600E, when positive, may also be helpful in excluding desmoplastic melanoma, and mechanistically suggests distinct molecular biology in nevoid and spindled cells, despite their combined resemblance to neurotized nevi.…”

Section: Discussionmentioning

confidence: 69%

Perineuriomatous nevi: A series of eight cases highlighting unifying pathologic features to avoid misdiagnosis

et al. 2021

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Background Perineuriomatous nevi are rare and diagnostically problematic. We report a series of eight perineuriomatous nevi to highlight the diagnostic features. Methods Cases were retrospectively reviewed and characterized. Results Median age was 42.5 years (range 25‐64), with equal sex distribution. Lesions occurred on the arm (n = 4), trunk (n = 2), and head/neck (n = 2). Median size was 7.5 mm (range 5‐12 mm). Clinical differential diagnoses included atypical nevus (3), blue nevus (1), neurofibroma (1), and dermatofibroma (1). Lesions were circumscribed, dome‐shaped (5/8), and biphasic (8/8) with nested epithelioid cells and wavy spindled cells arranged in whorled fascicles in a myxocollagenous stroma. When present, junctional growth was lentiginous (4/8). No cases displayed pleomorphism or mitotic figures. The perineuriomatous component stained positively for epithelial membrane antigen (8/8 focal to diffuse) and CD34 (4/5 focal to diffuse). SOX10 and S100 protein stained all nevoid cells and in some cases a subset of intermingled spindled cells in perineuriomatous areas, where other melanocytic markers were negative. p16 protein expression was uniformly retained (3/3), and p53 negative (0/2). Nevoid cells in most lesions were positive for BRAFV600E (5/7). Ki67 was mildly elevated (~5%) in 3/3 cases. Conclusions Recognizing the histopathologic and immunophenotypic features in these unusual nevi helps avoid overdiagnosis.

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Concurrent Ki-67 and p53 Immunolabeling in Cutaneous Melanocytic Neoplasms: An Adjunct for Recognition of the Vertical Growth Phase in Malignant Melanomas?

et al. 2000

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In attempts to extend morphologic observations in the prediction of biologic behavior, several adjunctive techniques have been applied to cutaneous melanocytic lesions of all types. These procedures have included nuclear morphometry (1); immunostaining for p53 proteins (2-4), the INK4A/CDKN2 tumor suppressor gene product (5), proliferating cell nuclear antigen (6 -8), or the Ki-67 nuclear protein (9 -13); and histochemical analysis of nucleolar organizer regions (14).A particularly challenging facet of dermatopathology concerns the more narrow setting of melanocytic malignancy. In that context, it is important to identify the growth phases (radial or vertical) of melanomas, in recognition of the prognostic importance attached to them (15). Our laboratory has

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Immunohistologic Evaluation of Putatively Mutant p53 Protein in Cutaneous Melanocytic Neoplasms

Cited by 5 publications

References 50 publications

Primary Melanoma of the Skin and Cutaneous Melanomatous Metastases

Primary Melanoma of the Skin and Cutaneous Melanomatous Metastases

Perineuriomatous nevi: A series of eight cases highlighting unifying pathologic features to avoid misdiagnosis

Concurrent Ki-67 and p53 Immunolabeling in Cutaneous Melanocytic Neoplasms: An Adjunct for Recognition of the Vertical Growth Phase in Malignant Melanomas?

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