Immunohistologic reactions of 20 monoclonal antibodies against non-A, non-B glycoconjugates with normal mucosae of the human gut

Torrado, Julio; Blasco, E.; Cosme, Ángel; Gutierrez-Hoyos, Ariel; Arenas, Juan; Cuadrado, Emilio

doi:10.1016/s0338-4535(87)80137-x

Cited by 6 publications

(3 citation statements)

References 5 publications

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“…Sections from each case were stained with hematoxylin and eosin for rou tine histological examination. [9,10]. Ulex europaeus lectin 1 labelled with rhodamine isothiocyanate TRITC Sigma L-4889 was used for testing the expres sion of H structure.…”

Section: Methodsmentioning

confidence: 99%

Expression of Lewis Antigenic Determinants in Colorectal Adenocarcinomas

Blasco

Torrado²,

Cosme³

et al. 1989

Pathobiology

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Expression of type 1 and type 2 chain Lewis antigens was studied in 32 rectal adenocarcinoma specimens; the results were correlated with the patients’ Lewis phenotype and secretor status. In addition, the pattern of expression of these antigens was analyzed in adjacent and distant normal mucosa. We used an indirect immunofluorescence technique with p-phenylenediamine counterstaining (Oriol technique) and a panel of monoclonal antibodies directed against the different antigenic specificities. Normal distal colonic mucosa only expresses monofucosylated structures (Le^a and X) arising from activity of the α1–3,4-fucosyltransferase coded by the Le gene. Rectal adenocarcinomas also show Le^a and X, but also reexpress blood group antigens ABH and exhibit difucosylated determinants (Le^b and Y). The accumulation of mono- and difucosylated type 2 chain in neoplastic processes, independently of the Le and Se genes, could be due to the enzymes coded by reactivation of the H and X genes. Blood group antigens form a complex signal code, genetically regulated, which intervenes in differentiation, growth and cellular recognition processes, and which may undergo important modifications during malignant transformation. These alterations could be useful in the diagnosis and prognosis of some types of carcinoma.

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Section: Methodsmentioning

confidence: 99%

Expression of Lewis Antigenic Determinants in Colorectal Adenocarcinomas

Blasco

Torrado²,

Cosme³

et al. 1989

Pathobiology

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“…Sections were rinsed with distilled water, placed in Tris-buffered saline (TBS) for 5 min and incubated for an hour at room temperature with monoclonal antibodies, using StrepABComplex/HRP DAKO 0492, against different blood group-related antigens. Some of these monoclonal antibodies were characterised in the First and Second International Workshop on Monoclonal Antibodies against Human Red Blood Cells and Related Antigens [5,21]. Other monoclonal antibodies, anti A (A581), anti B (582) and anti H (A583) from DAKO, were also used in the study.…”

Section: Methodsmentioning

confidence: 99%

“…Blood group-related antigens are oligosaccharides widely distributed in the body, particularly in epithelial cells [1][2][3][4][5][6][7]. In approximately 80% of people, these antigens are found in secretions (saliva, gastric juice) and these individuals are denominated "secretors".…”

Section: Introductionmentioning

confidence: 99%

Expression of blood group-related antigens in neoplastic uterine cervix

et al. 2008

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Expression of blood group antigens in normal, displastic and tumoral uterine cervix from 35 hysterectomised women with carcinoma of the cervix was investigated; the results were correlated with patients' ABH phenotype and secretor status. We used an indirect immunoperoxidase technique and a panel of monoclonal antibodies and lectins directed against different antigenic specificities. Anomalous expression of blood group antigens in premalignant lesions from cervix was found. Partial loos of expression of blood group antigens and some lectins in different grades of cervical intraepithelial neoplasia, and a total loss of expression in CIN III and in infiltrating carcinoma of the cervix from secretor patients was revealed. The findings herein described confirm the importance of these antigens as tumour markers and they might be useful for the study of cervical carcinogenesis.

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Lewis system alterations in gastric carcinogenesis

Torrado

Blasco

Gutierrez-Hoyos

et al. 1990

Cancer

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Alterations in the expression of type 1 blood group-related antigens (Lewis a and b) were examined immunohistochemically in 371 consecutives gastric biopsy and 80 surgical specimens from patients of gastric carcinoma. The ABH and Lewis phenotype and secretor status of the patients were correlated with histologic findings. An anomalous expression of Lewis a antigen was found in 88 of 249 gastric biopsy specimens of Lewis (a-b+) phenotype patients. The prevalence of this anomaly increased with the evolution of the premalignant process, in agreement with the commonly accepted model of gastric carcinogenesis. Thus, anomalous Lewis a antigen appeared in 66.6% of gastric dysplasia cases, in 64.6% of intestinal metaplasia, in 15.4% of atrophic gastritis, and in 7.4% of superficial gastritis. No alterations were found in subjects with normal gastric mucosa. Fortyseven of the 49 Lewis (a-b+) phenotype gastric carcinoma patients showed antigenic alterations in tumor cells (anomalous Lewis a antigen in 36 and loss of Lewis antigens in 11). In 26 of these gastric specimens an anomalous Lewis a antigen was present in areas of intestinal metaplasia and/or dysplasia away from the area of neoplastic transformation. The expression of Lewis a antigen in Lewis (a-b+) phenotype patients is a frequent phenomenon in gastric neoplastic cells and could result from the blocked synthesis of Lewis b antigen with accumulation of its precursors. These findings suggest that, during gastric carcinogenesis, antigenic alterations may precede neoplastic transformation. An anomalous Lewis a antigen could constitute a significant index of severity of the histologic lesion and contribute to identifying high-risk individuals.

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Immunohistologic reactions of 20 monoclonal antibodies against non-A, non-B glycoconjugates with normal mucosae of the human gut

Cited by 6 publications

References 5 publications

Expression of Lewis Antigenic Determinants in Colorectal Adenocarcinomas

Expression of Lewis Antigenic Determinants in Colorectal Adenocarcinomas

Expression of blood group-related antigens in neoplastic uterine cervix

Lewis system alterations in gastric carcinogenesis

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