Immunohistological analysis of Hodgkin's and Sternberg-Reed cells: Detection of a new antigen and evidence for selective IgG uptake in the absence of B cell, T cell and histiocytic markers

Abstract

To help clarify the origin and nature of Hodgkin's (H) and Sternberg-Reed (SR) cells, three different sets of experiments were performed. First, it was shown that cytoplasmic gamma, kappa, lambda occur not only in H and SR cells, but also in polymorphic tumor cells of epithelial, neurogenic, and lymphoid origin. Furthermore, human IgG that was injected i.v. into rats penetrated many rat liver cells, whereas injected human alpha 1-antitrypsin did not. Second, staining of frozen sections revealed that H and SR cells lack surface immunoglobulin and peripheral T-cell antigen. Third, an antiserum raised against the L 428 cell line (derived from Hodgkin's disease) and absorbed with human serum and normal cells did not react with any cells of tonsil tissue (lymphoid cells, histiocytes, and interdigitating reticulum cells), whereas it reacted strongly with the L 428 cell line cells and with H and SR cells of 10 different cases. In all ten cases, the antiserum stained the surface of H and SR cells; in two cases, it also stained the nucleoli and some chromatin spots in H and SR cells. The results obtained in these experiments indicate that H and SR cells are not closely related to lymphoid cells, histiocytes, or interdigitating reticulum cells. The findings also suggest that H and SR cells express one or more antigens that have not yet been detected on or in normal cells.

Functional expression of the eotaxin receptor CCR3 in CD30+ cutaneous T-cell lymphoma

“…[26][27][28][29] CD30 is a member of the tumor necrosis factor receptor family, which was originally described as Ki-1 antigen on Hodgkin and ReedSternberg cells in Hodgkin disease. 30,31 The favorable prognosis of CTCL may be related in part to the fact that dissemination to other body compartments occurs only late during disease development. This might be suggestive of chemotactic forces that keep lymphoma cells confined to the skin.…”

Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation

“…Peripheral blood lymphocytes were stimulated with phytohaemagglutinin as described by Takasaki et al (1981). At hours 0 and 96, aliquots of the cultures were pulse-labelled with [ 3H]-thymidine (Takasaki et al, 1981), and cytocentrifuge slides of unlabelled aliquots were prepared. HL-60 cell line (cf .…”

“…Ki-67 was obtained in studies aimed at the production of monoclonal antibodies to nuclear antigens specific to Hodgkin and Sternberg-Reed cells. The existence of such antigens was recently suggested (Stein et al, 1981) by the reactivity of conventional rabbit antiserum raised against the Hodgkin's disease-derived cell line LA28 (Schaadt et al, 1980). While most of the monoclonal antibodies generated against nuclei of the Hodgkin's cell line were reactive with nuclear structures of all cells tested, Ki-67 reacted with a nuclear antigen restricted to cells that are known to proliferate.…”

A Comprehensive Antibody Panel for Immunohistochemical Analysis of Formalin-Fixed, Paraffin-Embedded Hematopoietic Neoplasms of Mice: Analysis of Mouse Specific and Human Antibodies Cross-Reactive with Murine Tissue

“…Immunohistochemistry (IHC) has become an indispensable tool for the detailed immunophenotypic characterization of lymphomas and leukemias. In human pathology, the application of immunohistochemistry and immunofluorescence for the determination of the lineage of hematopoietic tumor cells began almost 30 years ago (Levy et al, 1977;Chu and MacDonald, 1979;Leong et al, 1979;Stein et al, 1980Stein et al, , 1981Bhoopat et al, 1989;Jaffe et al, 1999;Harris et al, 2000aHarris et al, , 2000b.…”