Immunohistological detection of gap junctions in human lymphoid tissue:                connexin43 in follicular dendritic and lymphoendothelial cells.

Krenács, Tibor; Rosendaal, Martin

doi:10.1177/43.11.7560895

Cited by 69 publications

(69 citation statements)

References 42 publications

(60 reference statements)

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“…High levels of connexin 43 are found in rapidly proliferating lymphoid and hematopoietic centers (Rosendaal et al, 1994;Krenacs and Rosendaal, 1995). In our previous study of factors influencing secretion (Head et al, 1997), decreasing dye coupling inversely increased proliferation.…”

Section: Dye Coupling (Figures 1 To 3)mentioning

confidence: 99%

Neuropeptides Exert Direct Effects on Rat Thymic Epithelial Cells in Culture

Head

Mentlein

Patay

et al. 1998

Journal of Immunology Research

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To determine if major thymic neuropeptides and neurotransmitters can directly influence the functional activity of cultured rat thymic epithelium, neuropeptides and neurotransmitters were applied, and intercellular communication, proliferation, and thymulin secretion assessed. After injections of a mixture of lucifer yellow dextran (too large to pass gap junctions) and cascade blue (which does) into single cells, some neuropeptides decrease dye coupling: 0.1 mM GABA (P < 0.0001), 100 nM NPY (P < 0.0001), 100 nM VIP (P < 0.001), 100 nM CGRP (P < 0.001), 100 nM SP (P < 0.01), and 0.1 mM histamine (P < 0.01), whereas 0.1 mM 5-HT, mM acetylcholine, and /xM isoproterenol (/3-adrenergic agonist) had no effect. Proliferation (incorporation of tritiated thymidine) was increased by CGRP (P 0.004) and histamine (P < 0.02), but decreased by isoproterenol (P 0.002), 5-HT (P 0.003), and acetylcholine (P < 0.05). The percentage of multinucleate cells was decreased after isoproterenol (2.5%), and increased after 5-HT (21.3%), GABA (15%), and histamine (15.1%). Compared to controls, thymulin in the supernatant was decreased after challenge with acetylcholine (52%), isoproterenol (71%), 5-HT (73%), and histamine (84%). This study demonstrates direct effects of neuropeptides and neurotransmitters on functional aspects of cultured thymic epithelial cells.

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Section: Dye Coupling (Figures 1 To 3)mentioning

confidence: 99%

Neuropeptides Exert Direct Effects on Rat Thymic Epithelial Cells in Culture

Head

Mentlein

Patay

et al. 1998

Journal of Immunology Research

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“…[1][2][3][4][5][6][7][8][9][10][11][12] Gap junctions have been comprehensively reviewed. 13 In 1995 and 1997, we proposed 1,14 that Cx43 gap junctions between stromal and hematopoietic cells determine hematopoiesis.…”

Section: Introductionmentioning

confidence: 99%

Hematopoietic capacity of connexin43 wild-type and knock-out fetal liver cells not different on wild-type stroma

Rosendaal

Jopling

2003

Blood

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“…The expression of Cxs by T, B, and NK cells derived from peripheral blood and secondary lymphoid organs has been reported [7][8][9][10][11][12]. Circulating lymphocytes express mainly Cx43, whereas expression of Cx40 occurs mainly in lymphocytes derived from secondary lymphoid organs [9].…”

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confidence: 99%

CD4+ T lymphocyte subsets express connexin 43 and establish gap junction channel communication with macrophages in vitro

Bermúdez-Fajardo

Ylihärsilä

Evans

et al. 2007

Journal of Leukocyte Biology

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Gap junction channels constructed of connexins (Cxs) are expressed by peripheral and secondary lymphoid organ-derived lymphocytes. These channels in the plasma membrane play key roles in a range of lymphocyte functions exemplified by the synthesis and secretion of Igs and cytokines and during transmigration across the endothelium. Most recently, their involvement in antigen crosspresentation has also been established. We report here for the first time the expression of mRNA and protein encoding Cx43 in mouse-derived CD4 + Th0, Th1, and Th2 lymphocyte subpopulations and demonstrate the establishment gap junction channel formation with primary macrophages in vitro. We show that this mode of direct communication is particularly favored in Th1-macrophage interactions and that LPS inhibits lymphocyte-macrophage cross-talk independently of the subset of lymphocyte involved. Our work suggests that gap junctionmediated communication can be modulated in the absence of specific antigenic stimulation. Therefore, a further mechanism featuring gap junction-mediated communication may be implicated in immune regulation. Keywords inflammation; intercellular; flow cytometry; lymphocytes Intercellular communication underpins cellular activation and various functions in mammals. In the hematopoietic and immune systems, direct cell-to-cell interactions influence cell phenotypic and functional characteristics such as those involved in blood formation or antigen-specific immune responses. These processes involve subsets of interacting cells, the surrounding signaling environment, and the functional outcomes of receptor-ligand interactions. One of the key channels underpinning intercellular communication are gap junctions, which are the focus of research by various groups who have shown their participation in leukocyte biology and the generation of immune responses [1][2][3][4]. Despite increasing evidence for the role of Cx proteins and gap junction channels in inflammatory and immunological reactions, their expression and specific functional roles remain to be studied in lymphocyte subsets with distinct functional properties. Here, we describe for the first time the differential expression of mRNA and protein encoding Cx43 in mousederived CD4 + Th lymphocyte subpopulations in vitro. We show that all of these cells can communicate with macrophages via gap junctions and that such cross-talk is particularly favored in Th1-macrophage interactions.A well-established methodology was used to obtain differentiated CD4 + Th1 and Th2 lymphocyte subpopulations in the absence of antigenic stimulation using Th0 (naïve) lymphocytes isolated from the spleen of specific pathogen-free (SPF) CD-1 male mice [13]. Naïve CD4 + T cells were obtained by negative selection from the spleens of 8-to 10-weekold SPF male CD-1 mice (Harlam, UK). B cells, NK cells, monocytes, and CD8 + T cells were removed by incubating splenocytes with rat anti-mouse CD19, CD11c, and CD8 mAb (Serotec, UK) and anti-CD16/32 (PharMingen, San Diego, CA, USA) before incubati...

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Immunohistological detection of gap junctions in human lymphoid tissue: connexin43 in follicular dendritic and lymphoendothelial cells.

Cited by 69 publications

References 42 publications

Neuropeptides Exert Direct Effects on Rat Thymic Epithelial Cells in Culture

Neuropeptides Exert Direct Effects on Rat Thymic Epithelial Cells in Culture

Hematopoietic capacity of connexin43 wild-type and knock-out fetal liver cells not different on wild-type stroma

CD4+ T lymphocyte subsets express connexin 43 and establish gap junction channel communication with macrophages in vitro

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