Immunohistological identification of cell subsets in human gingival after local treatment for gingivitis or periodontitis

Abstract

The cellular infiltrate present in human diseased gingiva was analyzed in biopsies from 12 patients with gingivitis or periodontitis. The samples studied had been obtained in the course of surgery at inflammatory sites remaining after institution of periodontal treatment. Histological and immunological techniques were used to identify macrophages, B-cells, plasma-cells, T-cells and T cell subsets, as well as cells expressing class II HLA membrane antigens. T-cells appeared as the predominant population, but plasma-cells were also visualized in nearly all samples. Both OKT4+ and OKT8+ cells were seen in all cases, the latter being more numerous in periodontitis patients. Interdigitating-like cells were observed, positively labelled for class II antigens, as well as macrophages which were more numerous in periodontitis patients. These results suggest the participation of all components of the immune response in gingival disease, in a way resembling chronic recurrent inflammatory diseases.

In situ characterization of mononuclear cells in marginal periodontitis of patients with Down's syndrome

“…This finding is in accordance with findings by Jully et al (40), who found more macrophages in periodontitis than in gingivitis of normal individuals.…”

Lymphocyte subpopulation in healthy and diseased gingival tissue

“…[83031] Polyclonal activation of B-cell by pathogenic bacteria results in the development of antibodies with low avidity, which results in inefficient elimination of bacteria,[11] thus, resulting in persistence of the bacteria which in turn could cause tissue destruction. Jully et al, [32] reported that progressive periodontitis was characterized by a B-cell or plasma cell lesion, which was in agreement with the present study. This may be due to hyperactivity influenced by an imbalance in regulatory T-lymphocytes which dominated the periodontal lesion.…”

“…These findings are consistent and in agreement with the previous reports by Jully et al and Richard et al [3233] The higher percentage of B-cell infiltration in the periodontitis group compared to other groups and a high ratio of plasma cell:B-cell represents an active infection which has initiated the adaptive immune response resulting in activation of B-cells and increased conversion of B-cells into immunoglobulin-producing plasma cells. Similar findings have been reported by Page and Schroeder, and Ogawa et al [3435]…”

“…This may be due to hyperactivity influenced by an imbalance in regulatory T-lymphocytes which dominated the periodontal lesion. [3233] Takahashi et al, [13] reported that activated B-cell or plasma cell produced immunoglobulins, with the light chain κ of the immunoglobulin being predominant in periodontitis lesions. Hence, κ light chain was chosen as a marker for activated B-cell or plasma cell.…”

Effect of Periodontal Therapy on Salivary Interleukin-12 Levels in Chronic Periodontitis

“…Successful periodontal therapy results clinically in shallow probing depths and a low tendency towards BOP and microbiologically, in a low detection level of periodontal pathogens [17]. In addition it reduces the size of inflamed lesion and causes a decrease in the periodontal tissues of inflammatory cell subsets, like B cells, T cells and plasma cells [18].…”