Immunohistological Studies of Metallothionein

Kojima, Yoko; Hamashima, Yoshihiro

doi:10.1267/ahc.13.277

Cited by 11 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In agreement with previous studies (18,19), metallothionein was absent from the glomeruli and other squamous epithelial cells. Metallothionein was also detected in certain cells of the intestine, lung, and testis.…”

Section: Resultssupporting

confidence: 93%

Immunochemical detection of metallothionein in specific epithelial cells of rat organs.

Danielson¹,

Ohi²,

Huang³

1982

Proc. Natl. Acad. Sci. U.S.A.

123

View full text Add to dashboard Cite

The distribution of a heavy metal binding protein, metallothionein, was studied immunocytochemically by using antimetallothionein antibody and the immunoperoxidase staining technique on histological sections of liver, kidney, intestine, lung, and testis from cadmium-treated rats. These tissues either accumulate heavy metals (e.g., liver, kidney, and testis) or are exposed to metal by ingestion or inhalation (intestine and lung). Staining for metallothionein was Because of the secretory, absorptive, or nutritive function of the metallothionein-localizing cells in the organs studied, we suggest that metallothionein may be involved in metal storage or transport in addition to its commonly proposed detoxification role.Metallothionein-a low molecular weight (Mr 6,100), sulfurrich protein-has been identified in a wide variety oforganisms ranging from yeast to man (1, 2). The molecule is characterized by a high cysteine content (20 of 61 amino acids in mammalian forms) and the ability to bind class II B transition metal atoms (e.g., Zn, Cd, or Hg). The precise biological function of metallothionein is not known, although it has been suggested to be important in metal homeostasis and detoxification because of its presence in many mammalian tissues (1-3). In addition, metallothionein synthesis has been shown to increase in the kidney (4), liver (4, 5), lung (6), and intestine (7) ofrodents after treatment with heavy metals. Stressful environmental conditions such as food restriction (8, 9), cold exposure (10), or physical exercise (10) have also been reported to increase metallothionein content in liver tissue. Furthermore, treatment ofcells in culture or experimental animals with glucocorticoid hormones can also induce metallothionein synthesis (9,11,12 MATERIALS AND METHODS Experimental Animals. Inbred male McCollum rats (6-8 mo of age; 450-500 g of body weight) were kept in animal quarters on a light/dark cycle (12 hr/12 hr) and were fed Purina chow and water ad lib. For induction ofmetallothionein synthesis the animals were injected subcutaneously with cadmium chloride in saline (1 mg/kg of body weight) once daily during the early afternoon for 4 consecutive days. The animals were sacrificed 48 hr after the last injection.Metallothionein. Rat metallothionein was isolated from liver and purified as described (13). Isometallothioneins A and B were mixed in equimolar proportions and crosslinked with glutaraldehyde prior to injection into rabbits for antibody production. The preparation and characterization of the metallothionein antibody have been described (13).Tissue Preparation. Experimental animals were sacrificed by cervical dislocation. Organs of interest were removed, washed briefly in isotonic saline, and fixed in Helly's fluid for 6-8 hr, except testis, which was fixed in Bouin's fixative for 24 hr. The tissue fragments were dehydrated by a series ofchanges in dioxane, transferred to toluene/terpineol (3:1), and embedded in paraffin. Sections were cut at 6-8 tum and mounted on slides previously coate...

show abstract

Section: Resultssupporting

confidence: 93%

Immunochemical detection of metallothionein in specific epithelial cells of rat organs.

Danielson¹,

Ohi²,

Huang³

1982

Proc. Natl. Acad. Sci. U.S.A.

123

View full text Add to dashboard Cite

show abstract

“…The metallothionein gene family expressed in the kidney and under basal conditions confined to the proximal tubule [14]. MT expression is also found in the developing kidney with expression developing the moment differentiation is completed from the S-body [14][15][16]. Since these populations appear to have progenitor cell-like properties and since the MT expression is relatively specific for proximal tubule cells, the MT gene family was assessed in HRTPT and HREC24T cells to determine whether these genes could be used to detect cross-talk between the two cell lines.…”

Section: Effect Of Prom1 and Cd24 Expression On The Cellular Response...mentioning

confidence: 99%

Primary and Immortalized Cultures of Human Proximal Tubule Cells Possess Both Progenitor and Non-Progenitor Cells That Can Impact Experimental Results

Shrestha

Haque

Ighofose

et al. 2023

JPM

View full text Add to dashboard Cite

Studies have reported the presence of renal proximal tubule specific progenitor cells which co-express PROM1 and CD24 markers on the cell surface. The RPTEC/TERT cell line is a telomerase-immortalized proximal tubule cell line that expresses two populations of cells, one co-expressing PROM1 and CD24 and another expressing only CD24, identical to primary cultures of human proximal tubule cells (HPT). The RPTEC/TERT cell line was used by the authors to generate two new cell lines, HRTPT co-expressing PROM1 and CD24 and HREC24T expressing only CD24. The HRTPT cell line has been shown to express properties expected of renal progenitor cells while HREC24T expresses none of these properties. The HPT cells were used in a previous study to determine the effects of elevated glucose concentrations on global gene expression. This study showed the alteration of expression of lysosomal and mTOR associated genes. In the present study, this gene set was used to determine if pure populations of cells expressing both PROM1 and CD24 had different patterns of expression than those expressing only CD24 when exposed to elevated glucose concentrations. In addition, experiments were performed to determine whether cross-talk might occur between the two cell lines based on their expression of PROM1 and CD24. It was shown that the expression of the mTOR and lysosomal genes was altered in expression between the HRTPT and HREC24T cell lines based on their PROM1 and CD24 expression. Using metallothionein (MT) expression as a marker demonstrated that both cell lines produced condition media that could alter the expression of the MT genes. It was also determined that PROM1 and CD24 co-expression was limited in renal cell carcinoma (RCC) cell lines.

show abstract

Prenatal Metabolism: Metals and Metallothionein

Miller

Shaikh

1983

Reproductive and Developmental Toxicity of Metals

View full text Add to dashboard Cite

Immunohistological Studies of Metallothionein

Cited by 11 publications

References 15 publications

Immunochemical detection of metallothionein in specific epithelial cells of rat organs.

Immunochemical detection of metallothionein in specific epithelial cells of rat organs.

Primary and Immortalized Cultures of Human Proximal Tubule Cells Possess Both Progenitor and Non-Progenitor Cells That Can Impact Experimental Results

Prenatal Metabolism: Metals and Metallothionein

Contact Info

Product

Resources

About