Immunohistological study in chordomas

Coindre, Jean‐Michel; Rivel, J; Trojani, M; Mascarel, Isabelle de; Mascarel, A. De

doi:10.1002/path.1711500110

Cited by 41 publications

(27 citation statements)

References 5 publications

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“…1,5,8,32,33 The results of our immunocytochemical analyses are in accordance with these findings. In both Cases 1 and 2 EMA and vimentin were expressed.…”

Section: Discussionsupporting

confidence: 87%

“…5,32,33,40 Immunostaining for NSE and ␣1-FP yield positive results in the majority of tumors. 1,5,8,32,34 Focal immunopositivity for S-100 and CEA was apparent in our two cases tested (Cases 1 and 2). In addition to immunocytochemistry, electron microscopy has significantly contributed to the characterization of chordomas.…”

Section: Discussionmentioning

confidence: 55%

“…Evident in the immunoprofile of chordoma cells is reactivity for LMW cytokeratins 7,8,18, and 19 (simple epithelium), as well as HMW 4, 5, and 6 (mucosal epithelia), 18,34,40 vimentin and EMA. 1,5,8,32,33 The results of our immunocytochemical analyses are in accordance with these findings.…”

Section: Discussionmentioning

confidence: 99%

“…The tumor cells contained abundant glycogen. Immunostains were positive for EMA, S-100 protein, vimentin, and for cytokeratins 5,8,18, and 19. The CEA preparations showed only mild focal immunoreactivity.…”

Section: Casementioning

confidence: 99%

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Intrasellar chordomas mimicking pituitary adenoma

Thodou¹,

Kontogeorgos²,

Scheithauer³

et al. 2000

FOC

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Whereas chordomas involving the sellar region are uncommon, largely or entirely intrasellar chordomas are rare. The authors report three cases in which the chordomas filled the pituitary fossa and presented as nonfunctioning pituitary adenomas. All lesions exhibited the typical histological patterns and immunophenotype of chordoma. One tumor, studied ultrastructurally and subjected to DNA analysis, was shown to have a diploid histogram. The authors present a clinicopathological study of these three cases and review the literature on intrasellar chordomas.

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“…1,5,8,32,33 The results of our immunocytochemical analyses are in accordance with these findings. In both Cases 1 and 2 EMA and vimentin were expressed.…”

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Casementioning

confidence: 99%

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Intrasellar chordomas mimicking pituitary adenoma

Thodou¹,

Kontogeorgos²,

Scheithauer³

et al. 2000

FOC

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“…The tumor cells are arranged in sheets, cords or single cells, and frequently reveal a multivacuolated cytoplasm, known as 'physaliphorous cells'. Immunohistochemically, the tumor cells co-express Pan-Cytokeratin (Pan-CK), CK8, CK19, EMA, S100 and vimentin (9)(10)(11). Brachyury (also known as T; MIM, 601397), a nuclear transcription factor with a key role in the formation of the posterior mesoderm and axial development (12), was recently presented as a specific and sensitive marker for chordomas (7,8,13).…”

Section: Introductionmentioning

confidence: 99%

Establishment and detailed functional and molecular genetic characterisation of a novel sacral chordoma cell line, MUG-Chor1

Rinner

Froehlich²,

Buerger³

et al. 2011

Int J Oncol

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Abstract. Chordomas are rare, low to intermediate grade malignant bone tumors of the axial skeleton. Current treatment options are limited to surgical procedures, as chordomas are largely resistant to conventional radiation and chemotherapy. Cell lines are valuable tools for exploring molecular mechan isms involved in tumorigenesis and they have a fundamental impact on the development of new anticancer agents. To date, only two chordoma cell lines exist world-wide. In the present study we report a third chordoma cell line, MUG-Chor1, as well as corresponding cultured fibroblasts established from a recur rent morphologically 'classic' sacrococcygeal chordoma of a 58-year-old Caucasian female. The cells are brachyury-positive and have the characteristics of chordoma. The genetic profile of the primary chordoma and the established chordoma cell line was investigated during the culturing period (early and late passage). MUG-Chor1 is karyotypically, <2n>43-47,XX,del(3) (q1?) [11], +7,del(9)(p1?),der(9;15)(q10;q10),-10,+der(12)t(9;12) (p2?;q1?),der (12)t(12;19)(p;p)t(17;19)(q;q),-15,der(17;21) (q10;q10),der (20)t(10;20) (q25?26?;q11?12?),-21,-22[20]/ idemx2[5] and displays known, chordoma-typical genetic changes, such as chromosomal gains at T/brachyury locus (6q27), losses at 9p24.3-p13.1 (includes the CDKN2a/CDKN2b locus), 10p15.3-q23.32 (includes the PTEN locus) and losses of 10q25.2 (includes the PDCD4 locus). MUG-Chor1 bears a marked resemblance to chordomas in vivo and is, therefore, an optimal in vitro chordoma model.

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