Immunohistological study in sarcoidosis: Evaluation at different sites of disease activity

Semenzato, Gianpietro; Pezzutto, Antonio; Pizzolo, Giovanni; Chilosi, Marco; Ossi, Elena; Angi, M; Cipriani, A

doi:10.1016/0090-1229(84)90004-7

Cited by 63 publications

(32 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They can modulate the influx of monocytes to the alveolar spaces and granulomas, in concert with immature monocyte-like macrophages, as shown in sarcoidosis by phenotype [62], and function [63]. Finally, CD4 positive T-cells accumulate during granuloma formation in tuberculosis [64], and sarcoidosis [65].…”

Section: Role Of Lymphocytesmentioning

confidence: 99%

From granuloma to fibrosis in interstitial lung diseases: molecular and cellular interactions

Mornex

Leroux²,

Greenland³

et al. 1994

Eur Respir J

View full text Add to dashboard Cite

show abstract

Section: Role Of Lymphocytesmentioning

confidence: 99%

From granuloma to fibrosis in interstitial lung diseases: molecular and cellular interactions

Mornex

Leroux²,

Greenland³

et al. 1994

Eur Respir J

View full text Add to dashboard Cite

show abstract

“…Anergy in response to recall antigens in skin tests for delayed type-hypersensitivity [1], impairment of in vitro proliferative responses of blood mononuclear cells to antigens and mitogens [2], and peripheral blood lymphocytopenia [3] are often seen together with infiltration of affected tissues with lymphocytes, particularly CD4 lymphocytes, and monocytes [4][5][6]. Before their migration to sites of active disease [7,8] sarcoid mononuclear cells must adhere to the endothelium of capillaries.…”

Section: Introductionmentioning

confidence: 99%

Increased CD11/CD18 expression on peripheral blood leucocytes of patients with sarcoidosis

Shakoor

Hamblin

1992

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYSarcoidosis is a tnultisystem disease of unknown etiology characterized by non-caseating granulomata, formed mainly from macrophages surrounded by lymphocytes and plasma cells. Using a novel method for the preparation of blood leucocytes for flow cytometry, we report increased expression of LeuCAMs (CD 11/CD 18) on peripheral blood leucocytes of 11 Caucasian and 10 Afro-Caribbean patients with sarcoidosis compared with age-, sex-and race-matched controls. Whilst the percentages ofthe cells expressing CDl 1/CD 18 were no different, the density, expressed as mean fluorescence intensity (MFI), was greater for all leucocytes in sarcoids than in normal individuals. The expression of intercellular adhesion molecule-1 (ICAM-1), a ligand for LFA-1 which is expressed on all leucocytes, was not significantly different from normal, whereas HLA-DR was expressed more intensely on sarcoid monocytes (/" < 001) and blood lymphocytes (P < 0 005) than control cells. Our findings are consistent with leucocyte activation although we were unable to confirm reports of elevated tumour necrosis factor-alpha (TNF-a) in the patients' plasma using an ELISA. Increased expression of adhesion molecules on peripheral blood leucocytes may play a role in the cellular extravasation, aggregation, and granuloma formation seen in sarcoidosis.

show abstract

“…The slides were counterstained with Hematoxylin, dehydrated and cleared as in routine processing. Cryostat sections were also stained with anti-immunoglobulin antibodies as previously reported [14], in all the biopsy specimens. Briefly, fluorescein-conjugated antibodies to human albumin, IgM, IgA, IgG, C3, C4, Clq and fibrinogen (Cappel Laboratories, Inc., Cochranville, Pa.) were used in direct immunofluorescence.…”

Section: Methodsmentioning

confidence: 99%

Immunopathology of ocular sarcoidosis

et al. 1990

Self Cite

View full text Add to dashboard Cite

Sarcoidosis is a multisystem disease characterized by enhanced immune responses at sites of involvement. To elucidate the immunopathogenesis of ophthalmic lesions, cell infiltrates in biopsies from conjunctiva and other tissues involved (lungs, lymph nodes, skin) were studied in 26 patients with active sarcoidosis in order to define the surface phenotype and the distribution of cells in granulomatous lesions. Biopsy specimens were also stained for detection of immunoglobulins, complement and fibrinogen deposits. The data demonstrate a lymphocytes/macrophages interaction in the central core of granulomatous areas as the crucial event that initiates the maintains the state of inflammation: at all sites of disease activity is present a compartmentalization of T-cells expressing a helper-related phenotype which account for the great majority of infiltrating cells both in the early lesions (aggregate of macrophages surrounded by lymphocytic infiltrate) and in well-organized sarcoid granulomata. The presence of plasma cells and immunoglobulin deposits may represent an epiphenomenon in line with the helper infiltration, suggesting a local hyper-reactivity of the B-cells immune system. This study suggests some immunopathogenetic mechanisms leading to the formation and growth of conjunctival sarcoid granulomata.

show abstract

Immunohistological study in sarcoidosis: Evaluation at different sites of disease activity

Cited by 63 publications

References 32 publications

From granuloma to fibrosis in interstitial lung diseases: molecular and cellular interactions

From granuloma to fibrosis in interstitial lung diseases: molecular and cellular interactions

Increased CD11/CD18 expression on peripheral blood leucocytes of patients with sarcoidosis

Immunopathology of ocular sarcoidosis

Contact Info

Product

Resources

About