Immunohistological Study of the Localization of Collagen Types I-XI in Bovine Term Placenta

Abstract

In order to determine the localization of extracellular matrix components in bovine term placenta, the tissue distributions of collagen types I to XI in the amnion, smooth chorion, villous chorion and villi were examined by indirect immunofluorescence microscopy. Collagen types I, III, V, and VI were found in the stroma of the smooth chorion, villous chorion and villi and in the compact layer of the amnion. Type II collagen, which was thought to be present only in cartilage, was found to be distributed in the epithelium of the amnion, stroma of the chorion and trophoblast of the villous chorion. Basement membrane components, type IV and VII collagens, were found to be located not only in the basement membrane but also in the epithelium and compact layer of the amnion. Additionally, type VI collagen was found to be located in the stroma of smooth chorion, and type VII collagen was found to be distributed in the trophoblast layer of the villous chorion. Types VIII, IX, X and XI collagens were found, for the first time, to be distributed in the trophoblast layer, amnion and chorionic epithelium as well as in the blood vessel walls of the chorion. Our results indicated that the compact layer of the amnion contained various types of collagen (I-IX) with various arrangements. However, the fibroblast layer of the amnion contained collagen types III, V and VI but type I collagen could not be observed. The blood vessel wall of the chorion contained all of the types of collagen examined in this study.

Perlecan Diversely Regulates the Migration and Proliferation of Distinct Cell Types in vitro

“…Since anti-perlecan antiserum was raised against the purified bovine kidney perlecan, which contains full-length perlecan, it reacts with various sites of perlecan to neutralize its multiple functions. Type I, III, IV, V and VI collagens and laminin were obtained from bovine placentae, as described by Lee et al [2000]. Briefly, bovine placentae were minced, washed with running water and then extracted with Tris-HCl buffer (pH 7.4) containing 10 m M ethylenediaminetetraacetic acid (EDTA; Kanto Chemical, Tokyo, Japan) and 0.5 M NaCl.…”

Diverse functions of perlecan in central nervous system cells in vitro

“…Bovine kidney perlecan, HS-truncated perlecan (Pln ΔHS) and anti-perlecan antiserum (anti-Pln) was prepared as previously described (Nakamura et al 2014). Type I/III collagens were isolated from bovine placentae as described by Lee et al (2000). Briefly, bovine placentae were minced, washed with running water, and treated with 4 mol/L guanidine chloride at 4°C for 48 h. Type I/III collagens were extracted from the insoluble fraction by digesting with 0.1% pepsin (Nacalai Tesque, Kyoto, Japan) at 25°C for 24 h under acidic conditions, and then were salted-out in 0.7 mol/L NaCl.…”

Functional role of type VI collagen during early feather development of the chicken embryo in vitro

“…The following four rabbit antisera were used as primary antibodies: antiporcine type I collagen (1:300 dilution, LB‐1196; LSL, Tokyo, Japan), antimouse type IV collagen (1:200 dilution, LB‐1403; LSL), antibovine type VI collagen (1:200 dilution, LB‐1697; LSL), and antichicken NCAM (1:1000 dilution, AB‐19013; Chemicon International, Temecula, CA, USA) polyclonal antibodies. The specificity of each antibody was confirmed by enzyme‐linked immunosorbent assay and western blotting (Lee et al . 2000).…”