Immunohystochemical analysis of CFTR in normal and disrupted spermatogenesis

Teixeira, Silvia A.; Sá, Rosália; Grangeia, Ana; Silva, Joaquina; Oliveira, Cristiano; Ferráz, Luís; Alves, Ângela; Paiva, Sandra; Barros, Alberto; Sousa, Mário

doi:10.3109/19396368.2012.718851

Cited by 19 publications

(15 citation statements)

References 28 publications

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“…Defective CFTR gene was observed in autosomal recessive disease of Cystic Fibrosis (CF) [12]. CFTR was found to be widely expressed in the male reproductive organs including testes [13] and epididymis [14]. This channel plays important role in optimizing the microenvironment of the epididymal fluid that is required for sperm maturation [15].…”

Section: Introductionmentioning

confidence: 99%

Testosterone regulates levels of cystic fibrosis transmembrane regulator, adenylate cyclase, and cAMP in the seminal vesicles of orchidectomized rats

et al. 2016

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Section: Introductionmentioning

confidence: 99%

Testosterone regulates levels of cystic fibrosis transmembrane regulator, adenylate cyclase, and cAMP in the seminal vesicles of orchidectomized rats

et al. 2016

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“…However, SOAT was recently shown to only localize in germ cells [325]. In other cases, in situ hybridization data have contradicted observations of immunohistochemistry, as noted for cystic fibrosis transmembrane conductance regulator (CFTR), which was localized in Sertoli cell cytoplasm [215,216,326]. In contrast, in situ hybridization gave a positive signal only in pachytene spermatocytes and spermatids [327].…”

Section: Mta1mentioning

confidence: 88%

Sertoli cell anatomy and cytoskeleton

Hess¹,

Vogl²

2015

Sertoli Cell Biology

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“…Mutations in CFTR have been linked with numerous abnormalities on the male reproductive system, including the congenital bilateral absence of the vas deferens and absence or abnormal production of sperm cells . CFTR is present mainly in the plasmatic membrane of SCs , playing a role in Cl − and HCO 3 − transport . In addition, it has been reported that CFTR also plays an important role in epithelial cells fluid volume regulation by interacting with water channel (aquaporins) (12).…”

Section: Introductionmentioning

confidence: 99%

“…Mutations in CFTR have been linked with numerous abnormalities on the male reproductive system, including the congenital bilateral absence of the vas deferens and absence or abnormal production of sperm cells (7)(8)(9). CFTR is present mainly in the plasmatic membrane of SCs (10), playing a role in Cl 2 and HCO 3 regulation (5) by interacting with water channel (aquaporins) (12). Aquaporins (AQPs) are crucial for water homeostasis as they facilitate rapid and massive water movement across epithelia (for review, see ref.…”

Section: Introductionmentioning

confidence: 99%

Aquaporin‐9 is expressed in rat Sertoli cells and interacts with the cystic fibrosis transmembrane conductance regulator

et al. 2014

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Men with mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene are usually subfertile/infertile. Besides playing a role in Cl 2 /HCO 3 2 transport, it has been proposed that CFTR interacts with water membrane transport systems, particularly aquaporins, to control seminiferous tubular secretion, which is regulated by the somatic Sertoli cells (SCs). As aquaporin-9 (AQP9) is highly expressed throughout the male reproductive tract, we hypothesized that it is also present in rat SCs and that it physically interacts with CFTR. To test this hypothesis, primary cultures of rat SCs were established, and expression of CFTR and AQP9 was assessed by RT-polymerase chain reactions (mRNA) and Western blot analysis (protein). A coimmunoprecipitation assay was used to evaluate the physical interaction between CFTR and AQP9. Our results show that CFTR and AQP9 are expressed in rat SCs. We were also able to detect a molecular interaction between CFTR and AQP9 in rat SCs. This is the first report describing the presence of AQP9, and its interaction with CFTR, in rat SCs. Moreover, our results provide evidence that CFTR is involved in water homeostasis of the seminiferous tubular secretion. These mechanisms may open new insights on therapeutic targets to counteract subfertility/infertility in men with cystic fibrosis and mutations in the CFTR gene.

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Immunohystochemical analysis of CFTR in normal and disrupted spermatogenesis

Cited by 19 publications

References 28 publications

Testosterone regulates levels of cystic fibrosis transmembrane regulator, adenylate cyclase, and cAMP in the seminal vesicles of orchidectomized rats

Testosterone regulates levels of cystic fibrosis transmembrane regulator, adenylate cyclase, and cAMP in the seminal vesicles of orchidectomized rats

Sertoli cell anatomy and cytoskeleton

Aquaporin‐9 is expressed in rat Sertoli cells and interacts with the cystic fibrosis transmembrane conductance regulator

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