Immunolatex visualisation of cell surface Forssman and polyamine antigens

Quash, G.; Niveleau, Alain; Aupoix, M.; Greenland, Timothy

doi:10.1016/0014-4827(76)90435-3

Cited by 36 publications

(8 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3), which is in accordance with our early results obtained on scanning electron microscopy showing the labelling of membrane PA sites (Quash et al 1976(Quash et al , 1978.…”

Section: Discussionsupporting

confidence: 94%

“…Previous studies from our group using scanning electron microscopy have allowed us to visualize the specific binding sites of anti-PA antibodies coupled to latex beads on the surfaces of cells in culture (Quash et al 1976(Quash et al , 1978. In addition to the immunochemical evidence for the existence of membrane PA sites, it has also been demonstrated that PA can interact with membrane proteins by covalent bonds mediated by the action of transglutaminases (TG) (Schrode and Folk 1978).…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Ultrastructural immunolocalization of polyamines in HeLa cells subjected to fast-freezing fixation and freeze substitution

Roch¹,

Nicolas

Quash³

1997

Histochemistry and Cell Biology

View full text Add to dashboard Cite

Polyamines have been localized at the ultrastructural level in HeLa cells subjected first to fast-freezing fixation (FFF)-freeze substitution (FS) and then to an immunocytochemical method combining anti-polyamine antibodies and immunogold labelling. Polyamines were found in both the cytoplasm and the nucleus and, in the latter, particularly over the dense chromatin area. To our knowledge, this is the first example of the electron microscopic localization of a hapten after FFF-FS. For the preservation of fine ultrastructural details, this FFF-FS method is not only adequate but also greatly reduces, if not totally eliminates, any leeching-out and redistribution of the polyamines during the preparation of the sample. For the preservation of antigenicity in situ during FS, epoxy resin was more effective than hydrophilic LR white resin, probably due to the solubility of polyamines in the latter.

show abstract

“…3), which is in accordance with our early results obtained on scanning electron microscopy showing the labelling of membrane PA sites (Quash et al 1976(Quash et al , 1978.…”

Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 98%

Ultrastructural immunolocalization of polyamines in HeLa cells subjected to fast-freezing fixation and freeze substitution

Roch¹,

Nicolas

Quash³

1997

Histochemistry and Cell Biology

View full text Add to dashboard Cite

show abstract

“…Changes in membrane proteins and membrane fluidity occur as myoblasts fuse to form postmitotic myotubes (Prives and Shinitzky, 1977) and the polycationic properties of polyamines could facilitate their interaction with phospholipids in cell membranes. The presence of putrescine binding sites on membranes has been reported by Quash et al (1976).…”

Section: Discussionmentioning

confidence: 89%

The role of polyamines in somatomedin‐stimulated differentiation of L6 myoblasts

Ewton

Erwin

Pegg

1984

Journal Cellular Physiology

View full text Add to dashboard Cite

The somatomedins are potent stimulators of proliferation and differentiation of cultured myoblasts. In studies on the mechanism(s) of these actions, we have measured the activities of ornithine decarboxylase (ODC), an enzyme associated with rapid cell proliferation, and creatine kinase (CK), a biochemical marker for muscle differentiation, after treatment of L6 myoblast cultures with Multiplication Stimulating Activity (MSA), a member of the somatomedin family of insulinlike growth factors. ODC levels reached a peak 24 hours after MSA addition (before any detectable differentiation of the myoblasts) and then decreased as differentiation commenced and CK activity increased. Addition of alpha-difluoromethylornithine (DFMO), an irreversible inhibitor of ODC, caused a dramatic decrease in differentiation. Measurement of 3H-thymidine incorporation, DNA content, and cell number established that the effect of DFMO on differentiation was not a simple consequence of its antiproliferative actions. Cellular levels of putrescine and spermidine (but not spermine) decreased substantially following addition of DFMO to the cultures. The inhibitory effects of DFMO were abolished upon addition of exogenous polyamines to the medium. However, addition of polyamines in the absence of MSA or DFMO did not mimic the stimulation of differentiation by MSA. We conclude that polyamines play an essential role in the stimulation of L6 myoblast differentiation by somatomedins, but they are not sufficient to effect this stimulation.

show abstract

“…Cells adhering to the eoverslips were fixed with 2.5 per cent glutaraldehyde in phosphate buffer pH 7, dehydrated and dried as already described (17). Cells were centrifuged at 500 × g for 3 minutes and resuspended in 3 ml of PBS.…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Latex fetuin spheres as probes for influenza virus neuraminidase in productively and abortively infected cells

Israël¹,

Niveleau

Quash

et al. 1979

Archives of Virology

View full text Add to dashboard Cite

Fetuin bound latex spheres do not adhere to the membranes of non-infected cells but adhere to those of cells productively infected by fowl plague virus (FPV Dobson strain). In contrast, asialo fetuin spheres do not attach to the membranes of productively infected cells. Moreover latex fetuin spheres incubated with extracts of productively infected cells and extensively washed are specifically enriched in neuraminidase activity without any trace of haemagglutinin. These observations suggest that viral neuraminidase in the membrane is the site of attachment of the sialic acid moieties of fetuin spheres. These neuraminidase sites are detectable when L cells are productively infected by a mammalian cell adapted mutant of the Dobson strain (FPV-B) but are not detectable on L cells abortively infected by wild type (FPV+). However, even in the abortive system, neuraminidase is synthesised de novo as shown by its labelling with 14C-glucosamine and by its isolation from labelled extracts of infected cells by latex fetuin spheres. These results show that misintegration of viral neuraminidase in the plasma membrane of L cells is a feature of abortive infection of these cells by the Dobson strain of FPV. However the relationship (if any) of this misintegration to abortive infection remains to be established.

show abstract

Immunolatex visualisation of cell surface Forssman and polyamine antigens

Cited by 36 publications

References 2 publications

Ultrastructural immunolocalization of polyamines in HeLa cells subjected to fast-freezing fixation and freeze substitution

Ultrastructural immunolocalization of polyamines in HeLa cells subjected to fast-freezing fixation and freeze substitution

The role of polyamines in somatomedin‐stimulated differentiation of L6 myoblasts

Latex fetuin spheres as probes for influenza virus neuraminidase in productively and abortively infected cells

Contact Info

Product

Resources

About