Immunoliposomal Systems Targeting Primary Effusion Lymphoma:
            <i>In Vitro</i>
            Study

Ruozi, Barbara; Riva, Giovanni; Belletti, Daniela; Tosi, Giovanni; Barozzi, Patrizia; Luppi, Mario; Forni, Flavio; Vandelli, Maria Angela

doi:10.2217/nnm.10.83

Cited by 8 publications

(10 citation statements)

References 39 publications

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“…Liposomes (LPs) were prepared using cationic lipid DOTAP by thin layer evaporation method (TLE) followed by extrusion (Ruozi et al, 2010). Briefly, cationic lipid (5 mg) was dissolved in 2 ml of chloroform.…”

Section: Cationic Liposomesmentioning

confidence: 99%

Functionalization of liposomes: microscopical methods for preformulative screening

Belletti

Vandelli

Tonelli

et al. 2014

Journal of Liposome Research

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The development of smart delivery systems able to deliver and target a drug to the site of action is one of the major challenges in the field of pharmaceutical technology. The surface modification of nanocarriers, such as liposomes, is widely investigated either for increasing the blood circulation time (by pegylation) or for interacting with specific tissues or cells (by conjugation of a selective ligand as a monoclonal antibody, mAb). Microscopical analysis thereby is a useful approach to evaluate the morphology and the size owing to resolution and versatility in defining either surface modification or the architecture and the internal structure of liposomes. This contribution aims to connect the outputs obtained by transmission electron (TEM) and atomic force (AFM) microscopical techniques for identifying the modifications on the liposomal surface. To reach this objective, we prepared liposomes applying two different pegylation technologies and further modifying the surface by mAb conjugation. This work demonstrates the feasibility to apply the combined approach (TEM and AFM analysis) in the evaluation of the efficacy of a surface engineering process.

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Section: Cationic Liposomesmentioning

confidence: 99%

Functionalization of liposomes: microscopical methods for preformulative screening

Belletti

Vandelli

Tonelli

et al. 2014

Journal of Liposome Research

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“…They have many advantages, including safety, stability, and lower immunogenicity 5, 6. Currently, the major types of non‐viral gene delivery vectors are cationic liposomes and cationic biomaterials 7, 8…”

Section: Introductionmentioning

confidence: 99%

“…5,6 Currently, the major types of non-viral gene delivery vectors are cationic liposomes and cationic biomaterials. 7,8 Biomaterials-based delivery platforms are gaining attention for their potential to control spatiotemporal distributions. Many efforts have been made to design new drug carrier systems that are able to improve the drug's solubility, stability, and target in vivo, while retaining its anticancer therapeutic efficacy.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and efficient hepatocyte targeting of galactosylated chitosan as a gene carrier in vitro and in vivo

et al. 2011

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While chitosan (CS) has been researched widely as a non-viral vector, its usefulness has been limited by its low cell specificity and transfection efficiency. Therefore, we successfully synthesized galactosylated chitosan (GC) and complexed it with an enhanced green fluorescent protein plasmid (pIRES-EGFP) for transfection into cultured H22 cells (murine hepatic cancer cell line) using various GC/EGFP (N/P) charge ratios. Maximal gene transfection rates detected by flow cytometry occurred at an N/P ratio 5:1. Compared with those of lipofectin/EGFP and naked pIRES-EGFP, GC/EGFP complexes show a very efficient cell-selective transfection to hepatocytes. The MTT assay detected relatively low cytotoxicity in cells transfected with GC. A recombinant plasmid granulocyte-macrophage colony-stimulating factor (GM-SCF) and interleukin (IL) 21 (pIRES/GM-CSF-IL21) was successfully constructed and GC/GM-CSF-IL21 nanoparticles (average diameter, 82.1 nm) were administered via the tail vein of mice with liver metastasis of colon cancer model, for 5 consecutive days. The GC/GM-CSF-IL21 nanoparticles exhibited hepatocyte and passive tumor specificity, increased therapeutic efficacy compared to control groups, promoted leukocytes to aggregate in tumor tissues, and activated the cytotoxicity of natural killer (NK) cells and cytolytic T lymphocyte (CTL). Our results indicate that GC can be used in gene therapy to improve transfection efficiency and can be used as an immunological stimulant in vivo.

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“…9 Aiming to expand RNAi strategies for PEL treatment, we previously tested different lipid-based nanocarriers for their ability to target PEL cell lines efficiently. [10][11][12] In the present work, we investigated the in vitro and in vivo antineoplastic activity associated with liposomal siRNA-mediated knockdown of the BLIMP1/PRDM1 gene, which encodes Blimp-1, a transcription factor considered a crucial regulator of the transcriptional network in post-germinal center B-cell stages.…”

mentioning

confidence: 99%

“…[10][11][12] The same liposomes, either loaded with scrambled oligonucleotides (i.e. mock siRNA, commercially provided together with validated siRNA) or empty (vehicle), as well as free anti-BLIMP1 siRNA without a vehicle, were used as negative controls.…”

mentioning

confidence: 99%

Antineoplastic effects of liposomal short interfering RNA treatment targeting BLIMP1/PRDM1 in primary effusion lymphoma

et al. 2015

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Immunoliposomal Systems Targeting Primary Effusion Lymphoma: In Vitro Study

Abstract: ILp could be proposed as effective carriers for oligonucleotide transfer in BCBL-1 cells. In vitro experimental results encourage us to further test the in vivo therapeutic potential of ILp for specific delivery of antitumoral agents.

Cited by 8 publications

References 39 publications

Functionalization of liposomes: microscopical methods for preformulative screening

Functionalization of liposomes: microscopical methods for preformulative screening

Synthesis and efficient hepatocyte targeting of galactosylated chitosan as a gene carrier in vitro and in vivo

Antineoplastic effects of liposomal short interfering RNA treatment targeting BLIMP1/PRDM1 in primary effusion lymphoma

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