Immunolocalization of Cancer Stem Cells Marker ALDH1 and its Association with Tumor Budding in Oral Squamous Cell Carcinoma

Júnior, Helvécio Marangon; Melo, Victória Vasconcellos Moreira; Caixeta, Ângela Braga; Souto, Giovanna Ribeiro; Souza, Paulo Eduardo Alencar; Aguiar, Maria Cássia Ferreira de; Horta, Martinho Campolina Rebello

doi:10.1007/s12105-018-0985-4

Cited by 22 publications

(27 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, high ALDH1 expression was recently associated with a high intensity of tumor budding. This reinforces the role of CSCs in carcinogenesis through ALDH1 expression, which can accelerate the invasion of cancer cells and, consequently, worsen the biological tumor behavior ( 28 ).…”

Section: Discussionsupporting

confidence: 63%

Overexpression of ALDH1 and EMT marker profile are linked with unfavorable outcome in head and neck cancer

Vieira¹,

Campos²,

Jesus³

et al. 2020

Med Oral

View full text Add to dashboard Cite

Background The aim of this research was to assess the expression of aldehyde dehydrogenase 1 (ALDH1) and epithelial-mesenchymal transition (EMT) markers in head and neck squamous cell carcinoma (HNSCC), and to correlate them with the clinical and histopathological parameters of a patient cohort with follow-up over an 8-year period. Material and Methods For this, seventeen HNSCC and non-neoplastic adjacent epithelium (AE) samples were subjected to laser microdissection and real-time PCR to evaluate the mRNA expression of ALDH1, E-cadherin (E-CAD), N-cadherin (N-CAD), and vimentin (VIM). Also, immunohistochemistry was performed for ALDH1, E-CAD, N-CAD, and VIM in the tumor center (TC), invasion front (IF), and AE of the seventeen samples. Mann-Whitney, Kruskal-Wallis and Chi-square tests were used to correlate the mRNA and immunohistochemical expression with different variables, considering p <0.05. Kaplan-Meier curves were produced for local recurrence, regional metastasis and treatment. Results A mRNA overexpression of ALDH1 in primary tumors was associated with regional metastasis and a high ALDH1 immunostaining was related to metastasis and a worse patient outcome. Additionally, a favorable outcome was associated with the transition phase and an unfavorable outcome was associated with EMT event. An overall 26.9 months was observed with longer survival associated with surgery and radiotherapy. Conclusions However, due to the intense variability inherent to the indicator proteins in the EMT process, the complete profile markers related to this biological process should be continuous investigated. Key words: Aldehyde dehydrogenase 1, epithelial mesenchymal transition, head and neck cancer, squamous cell carcinoma, follow-up study.

show abstract

Section: Discussionsupporting

confidence: 63%

Overexpression of ALDH1 and EMT marker profile are linked with unfavorable outcome in head and neck cancer

Vieira¹,

Campos²,

Jesus³

et al. 2020

Med Oral

View full text Add to dashboard Cite

show abstract

“…This is followed by migration of cancer cells away from the main tumor body toward deeper parts of the affected organ and in some instances by various routes toward distant organs (ie, metastasis). In oral squamous cell carcinoma (OSCC), a recent study by Marangon Junior et al 13 in tumors having a high density of budding, the expression of ALDH1 was higher in the area of buds than in areas outside tumor budding. In a three-dimensional assessment of tumor budding, 6 however, cancer cell invasion was reported as a collective migration of cells, and migration of single cancer cells was deemed rare.…”

Section: Tumor Budding Invasion and Metastasismentioning

confidence: 98%

“…In nasopharyngeal carcinoma, Luo et al 9 reported that tumor budding cells had a high expression of the cancer stem cell marker, aldehyde dehydrogenase 1 (ALDH1), and proposed that budding cells might have the invasive and metastatic characteristics of the cancer stem cells. In oral squamous cell carcinoma (OSCC), a recent study by Marangon Junior et al 13 in tumors having a high density of budding, the expression of ALDH1 was higher in the area of buds than in areas outside tumor budding. They explained this pattern of ALDH1 expression as a consequence of the cancer cells forming the buds having phenotypic properties of cancer stem cells.…”

Section: Tumor Budding Invasion and Metastasismentioning

confidence: 98%

Hallmarks of cancer: Tumor budding as a sign of invasion and metastasis in head and neck cancer

et al. 2019

View full text Add to dashboard Cite

Invasion and metastasis are hallmarks of cancer. The concept of tumor budding at tumor‐host interface has been documented in many carcinomas. A growing body of evidence indicates that tumor budding is a sign of invasion and early step for metastasis of many epithelial cancers including head and neck squamous cell carcinoma (HNSCC). In addition, recent research has underlined the importance of tumor budding as a promising prognosticator in HNSCC. This review summarizes the findings regarding tumor budding in HNSCC and focuses on the role of tumor budding in invasion and metastasis. Also, we highlight the prognostic significance of tumor budding in HNSCC and its potential for improving clinical decision making in terms of recommending optimal individualized treatment for this patient population.

show abstract

“…Cancer stem cells (CSC) are considered to be the main culprit of tumor local invasion and metastasis ( Marangon Junior et al, 2019 ). Tumor budding is known to be a morphological marker of tumor invasion.…”

Section: Introductionmentioning

confidence: 99%

“…Tumor budding is known to be a morphological marker of tumor invasion. Additionally, it has been suggested that tumor buds may have “stem cell” characteristics ( Marangon Junior et al, 2019 ). In our study, we found an association between tumor buds and CSCs.…”

Section: Introductionmentioning

confidence: 99%

Tumor budding of cervical squamous cell carcinoma: epithelial-mesenchymal transition-like cancer stem cells?

Zheng

Xie

et al. 2022

PeerJ

View full text Add to dashboard Cite

Recent evidence indicates that cancer stem cells (CSCs) are the origin of cancers. Scientists have identified CSCs in various tumors and have suggested the existence of a variety of states of CSCs. The existence of epithelial–mesenchymal transition (EMT)-like CSCs has been confirmed in vitro, but they have not been identified in vivo. Tumor budding was defined as single cell or clusters of ≤ 5 cells at the invasive front of cancers. Such tumor budding is hypothesized to be closely related to EMT and linked to CSCs, especially to those migrating at the invasive front. Therefore, tumor budding has been proposed to represent EMT-like stem cells. However, this hypothesis has not yet been proven. Thus, we studied the expression of EMT markers, certain CSC markers of tumor budding, and the tumor center of cervical squamous cell carcinoma (CxSCC). We performed tissue chip analyses of 95 primary CxSCCs from patients. Expression of EMT and CSC markers (E-cadherin, β-catenin, vimentin, Ki67, CD44, SOX2 , and ALDH1A1) in a set of tumor samples on tissue chips (87 cases of tumor budding/the main tumor body) were evaluated by immunohistochemistry. We found that the cell-membranous expression of β-catenin was stronger in the main tumor body than in tumor buds. Compared with the main tumor body, tumor buds had reduced proliferative activity as measured by Ki67. Moreover, vimentin expression was high and E-cadherin expression was low in tumor buds. Expression of EMT-related markers suggested that tumor buds were correlated with EMT. We noted that CxSCC tumor buds had a CD44negative/low/SOX2high/ALDH1A1high staining pattern, indicating that tumor buds of CxSCC present CSC-like immunophenotypic features. Taken together, our data indicate that tumor buds in CxSCC may represent EMT-like CSCs in vivo.

show abstract

Immunolocalization of Cancer Stem Cells Marker ALDH1 and its Association with Tumor Budding in Oral Squamous Cell Carcinoma

Cited by 22 publications

References 36 publications

Overexpression of ALDH1 and EMT marker profile are linked with unfavorable outcome in head and neck cancer

Overexpression of ALDH1 and EMT marker profile are linked with unfavorable outcome in head and neck cancer

Hallmarks of cancer: Tumor budding as a sign of invasion and metastasis in head and neck cancer

Tumor budding of cervical squamous cell carcinoma: epithelial-mesenchymal transition-like cancer stem cells?

Contact Info

Product

Resources

About