Immunolocalization of Cytokines and Growth Factors in Oral Submucous Fibrosis

Haque, MF; Harris, Malcolm; Meghji, S; Barrett, A.W.

doi:10.1006/cyto.1997.0342

Cited by 110 publications

(113 citation statements)

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“…Consistent with the above finding overexpression of p53 in the nuclei of epithelial cells of PHT induced GO tissues have been observed (Saito, 1999). Both the overexpression of mitogenic factors and p53 has been observed in oral dysplastic and precancerous lesions, and has been frequently associated with the development of a wide range of carcinomas including OSCCs (Haque, 1998;Wakulich, 2002;Abbas, 2007;Angiero, 2008;Bishen, 2008;Pai, 2009;). Incidentally, cyclosporine-an immunosuppressant and nifedepine-a calcium channel blocker induce GO and OSCCs have been reported to arise from these tissues as well (Varga, 1991;Pahor, 1996).…”

Section: Introductionsupporting

confidence: 69%

Lack of Mutation in p53 and H-ras Genes in Phenytoin Induced Gingival Overgrowth Suggests its Non Cancerous Nature

Jayaraman¹,

Valiathan²,

Jayakumar³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Background: There have been case reports of oral squamous cell carcinoma arising from gingival overgrowth induced by phenytoin -an antiepileptic drug. However, a detailed analysis for the presence of mutations in p53 and ras genes, which are the two most frequently mutated genes in cancers, in phenytoin induced gingival overgrowth tissues has hitherto not been performed. Methods: Cellular DNA isolated from twenty gingival overgrowth tissues collected from patients undergoing phenytoin therapy were amplified using primers for p53 (exons 5-8) and H-ras (exons 1-2) genes. The PCR amplicons were then gel purified and subjected to direct sequencing analysis to screen for mutations. Results: Direct sequencing of twenty samples of phenytoin induced gingival growth did not identify mutations in any of the exons of p53 and H-ras genes that were analyzed. Conclusion: Our result indicates that mutational alteration of p53 and H-ras genes is infrequent in phenytoin induced gingival growth, which thus suggests a non malignant nature of this pathology. The findings in the present study are clinically significant as a large number of epileptic patients are treated with phenytoin.

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Section: Introductionsupporting

confidence: 69%

Lack of Mutation in p53 and H-ras Genes in Phenytoin Induced Gingival Overgrowth Suggests its Non Cancerous Nature

Jayaraman¹,

Valiathan²,

Jayakumar³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…Thus, it might be excessive COMP secreted by fibroblasts that subsequently facilitate the deposition of collagen in OSF and promote the development of OSF by binding to matrix components. Moreover, the increased expression of fibrogenic cytokines, namely TGF-h, was found in OSF tissues (30), whereas a previous study also indicated that COMP could be induced to overexpress by TGF-h treatment (29), suggesting that increased TGF-h might also provoke the synthesis of COMP in OSF. In addition, autoimmunity has been examined as an etiologic factor for OSF (31), whereas COMP has been cited as potential autoantigens in patients with rheumatoid arthritis (32).…”

Section: Discussionmentioning

confidence: 78%

Discovery of Novel Biomarkers in Oral Submucous Fibrosis by Microarray Analysis

Jian

et al. 2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Oral submucous fibrosis (OSF) is a high-risk precancerous condition of the oral cavity. Areca nut chewing is its key etiologic factor, but the full pathogenesis is still obscure. In this study, microarray analysis was used to characterize the mRNA changes of 14,500 genes in four OSF and four normal buccal mucosa samples to identify novel biomarkers of OSF. Five candidate genes with the most differential changes were chosen for validation. The correlation between clinicopathologic variables of 66 OSF patients and the expression of each gene was assessed by immunohistochemistry. The microarray analysis showed that 661 genes were up-regulated (fold value >2) and 129 genes were downregulated (fold value <0.5) in OSF (q < 0.01). The top three up-regulated genes [Loricrin, Cartilage oligomeric matrix protein (COMP), Cys-X-Cys ligand 9 (CXCL9)] with the largest fold changes and the top two downregulated genes [keratin 19 (KRT19), cytochrome P450 3A5 (CYP 3A5)] with the most significantly differential changes in OSF were chosen as candidate biomarkers. In immunohistochemical results, the expression of Loricrin and COMP showed statistically significant association with histologic grade of OSF (P = 0.03 and 0.006, respectively). COMP was found to be overexpressed frequently in patients with the habit of areca nut chewing for more than 4 years (P = 0.002). CYP 3A5 was revealed an inverse correlation with histologic grade (P = 0.04). This pilot study showed that five novel genes might play important roles in the pathogenesis of OSF and may be clinically useful for early detection of OSF. (Cancer Epidemiol Biomarkers Prev 2008;17(9):2249 -59)

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“…This unchecked cellular signaling results in the proliferation of fibroblasts, increased collagen synthesis, and suppression of collagenase production, with the overall effect of restructuring the local ECM. 42 These effects suggest that techniques that target collagen remapping might provide direct diagnostic coupling of the spectra to the pathology. Figure 7(a) shows that this spectral region, up to 420 nm, was the most prominent region in explaining the variance when using DLIPS and is in the range for emission due to collagen.…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of differential laser-induced perturbation spectroscopy as a technique to discriminate emerging skin pathology

et al. 2012

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Abstract. Fluorescence spectroscopy has been widely investigated as a technique for identifying pathological tissue; however, unrelated subject-to-subject variations in spectra complicate data analysis and interpretation. We describe and evaluate a new biosensing technique, differential laser-induced perturbation spectroscopy (DLIPS), based on deep ultraviolet (UV) photochemical perturbation in combination with difference spectroscopy. This technique combines sequential fluorescence probing (pre-and post-perturbation) with sub-ablative UV perturbation and difference spectroscopy to provide a new spectral dimension, facilitating two improvements over fluorescence spectroscopy. First, the differential technique eliminates significant variations in absolute fluorescence response within subject populations. Second, UV perturbations alter the extracellular matrix (ECM), directly coupling the DLIPS response to the biological structure. Improved biosensing with DLIPS is demonstrated in vivo in a murine model of chemically induced skin lesion development. Component loading analysis of the data indicates that the DLIPS technique couples to structural proteins in the ECM. Analysis of variance shows that DLIPS has a significant response to emerging pathology as opposed to other population differences. An optimal likelihood ratio classifier for the DLIPS dataset shows that this technique holds promise for improved diagnosis of epithelial pathology.Results further indicate that DLIPS may improve diagnosis of tissue by augmenting fluorescence spectra (i.e. orthogonal sensing).

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Immunolocalization of Cytokines and Growth Factors in Oral Submucous Fibrosis

Cited by 110 publications

References 0 publications

Lack of Mutation in p53 and H-ras Genes in Phenytoin Induced Gingival Overgrowth Suggests its Non Cancerous Nature

Lack of Mutation in p53 and H-ras Genes in Phenytoin Induced Gingival Overgrowth Suggests its Non Cancerous Nature

Discovery of Novel Biomarkers in Oral Submucous Fibrosis by Microarray Analysis

Comparative evaluation of differential laser-induced perturbation spectroscopy as a technique to discriminate emerging skin pathology

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