Immunolocalization of glucose transporter 1 and 3 in the placenta: Application to cytodiagnosis of Papanicolaou smear

Sato, Misako; Nakamura, Yasushi; Sogawa, T.; Yang, Qifeng; Taniguchi, Takeshi; Taniguchi, Emiko; Kagiya, Tomoko; Nakamura, Misa; Mori, Ichiro; Kakudo, Kennichi

doi:10.1002/dc.10124

Cited by 7 publications

(5 citation statements)

References 16 publications

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“…A finding of this study that may further explain variable expression of GLUT3 was the high degree of GLUT3 localization in the term placentalcytotrophoblast cells (CTBs). This is similar to the findings of multiple investigators who have observed GLUT3 expression exclusively in the CTBs of human placenta in early pregnancy [43–45]. CTBs contain stem cells, which differentiate to become non-proliferative multinucleated STBs with cell fusion.…”

Section: Discussionsupporting

confidence: 91%

Placental glucose transporter 3 (GLUT3) is up-regulated in human pregnancies complicated by late-onset intrauterine growth restriction

et al. 2013

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Introduction Transport of glucose from maternal blood across the placental trophoblastic tissue barrier is critical to sustain fetal growth. The mechanism by which GLUTs are regulated in trophoblasts in response to ischemic hypoxia encountered with intra-uterine fetal growth restriction (IUGR) has not been suitably investigated. Objective To investigate placental expression of GLUT1, GLUT3 and GLUT4 and possible mechanisms of GLUT regulation in idiopathic IUGR. Methods We analyzed clinical, biochemical and histological data from placentas collected from women affected by idiopathic full-term IUGR (n=10) and gestational age-matched healthy controls (n=10). Results We found increased GLUT3 protein expression in the trophoblast (cytotrophoblast greater than syncytiotrophoblast) on the maternal aspect of the placenta in IUGR compared to normal placenta, but no differences in GLUT1 or GLUT4 were found. No differential methylation of the GLUT3 promoter between normal and IUGR placentas was observed. Increased GLUT3 expression was associated with an increased nuclear concentration of HIF-1α, suggesting hypoxia may play a role in the up-regulation of GLUT3. Discussion Further studies are needed to elucidate whether increased GLUT3 expression in IUGR is a marker for defective villous maturation or an adaptive response of the trophoblast in response to chronic hypoxia. Conclusions Patients with IUGR have increased trophoblast expression of GLUT3, as found under the low-oxygen conditions of the first trimester.

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Section: Discussionsupporting

confidence: 91%

Placental glucose transporter 3 (GLUT3) is up-regulated in human pregnancies complicated by late-onset intrauterine growth restriction

et al. 2013

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show abstract

“…A finding of this study that may further explain variable expression of GLUT3 was the high degree of GLUT3 localization in the term placentalcytotrophoblast cells (CTBs). This is similar to the findings of multiple investigators who have observed GLUT3 expression exclusively in the CTBs of human placenta in early pregnancy [43][44][45]. CTBs contain stem cells, which differentiate to become non-proliferative multinucleated STBs with cell fusion.…”

Section: Discussionsupporting

confidence: 91%

839: Placental glucose transporter 8 (GLUT8) is up-regulated in human pregnancies complicated by late-onset intrauterine growth restriction

Lei

Perry

Shin

et al. 2015

American Journal of Obstetrics and Gynecology

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“…Because other GLUTs were demonstrated to have expression in endometrium, [27][28][29][30] and because GLUT8 appeared to be hormonally regulated, 27,30,31 we hypothesized that GLUT1 and GLUT8 would be overexpressed in endometrial cancer compared to benign endometrial tissue, controlled for menopausal status. As with the upregulation of glucose transporters in other cancers, the upregulation of GLUT1 and GLUT8 may identify poorly differentiated, thus more aggressive endometrial tumors.…”

Section: Discussionmentioning

confidence: 99%

GLUT1 and GLUT8 in endometrium and endometrial adenocarcinoma

et al. 2006

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Glucose is provided to cells by a family of glucose transport facilitators known as GLUTs. These transporters are expressed in a tissue specific manner and are overexpressed in many primary tumors of these tissues. Regulation of glucose transport facilitator expression has been demonstrated in endometrial tissue and endometrial adenocarcinoma. The following experiments were conducted to quantify and localize the expression of GLUT1 and GLUT8 in benign endometrium and compare this expression to endometrial cancer. Endometrial tissue samples were obtained from random hysterectomy specimens of patients with benign indications for surgery and endometrial cancer. Immunoblot and immunolocatization studies were performed using GLUT1 and GLUT8 specific antisera. Endometrial samples from 65 women who had undergone hysterectomy were examined (n ¼ 38 benign, n ¼ 27 malignant). A 44 and a 35.4 kDa immunoreacive species was demonstrated in endometrium and endometrial cancer for GLUT1 and GLUT8, respectively. Upregulation of GLUT1 expression was demonstrated with increasing grade of tumors (Po0.002). GLUT8 expression was increased in all tumor subtypes compared to atrophic endometrium (Po0.001). Apical localization by GLUT1 and GLUT8 was demonstrated in endometrial glands. GLUT1 and GLUT8 demonstrated diffuse intracellular localization in the cancer subtypes. GLUT1 and GLUT8 are expressed in both human endometrium and endometrial cancer. There appears to be a step-wise progression in GLUT1 and GLUT8 expression as tumor histopathology worsens. GLUT1 and GLUT8 may be important markers in tumor differentiation, as well as providing energy to rapidly dividing tumor cells.

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Immunolocalization of glucose transporter 1 and 3 in the placenta: Application to cytodiagnosis of Papanicolaou smear

Cited by 7 publications

References 16 publications

Placental glucose transporter 3 (GLUT3) is up-regulated in human pregnancies complicated by late-onset intrauterine growth restriction

Placental glucose transporter 3 (GLUT3) is up-regulated in human pregnancies complicated by late-onset intrauterine growth restriction

839: Placental glucose transporter 8 (GLUT8) is up-regulated in human pregnancies complicated by late-onset intrauterine growth restriction

GLUT1 and GLUT8 in endometrium and endometrial adenocarcinoma

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