Immunolocalization of heat shock proteins 27 and 47 during repair of induced oral ulcers

Abstract

Heat shock proteins (Hsps) 27 and 47are involved in the control of apoptosis, cell migration, and collagen synthesis. There is some understanding of the immunolocalization of these proteins during the repair process in skin and gastrointestinal mucosa, but their expressions in normal and injured oral mucosa are unknown. The aim of this study was to analyze the immunolocalization and intensity of these proteins in oral ulcers induced in rats and to compare these expression levels with those reported in skin and gastric mucosa. Ulcers were induced on the ventral surface of the tongues of rats. The rats were then euthanized at 0, 24, 48, 72, and 120 h. Hsp27 expression remained low in the first hours of repair, but was higher at 72 h, mainly in the migrating epithelium. Expression of Hsp47 was high at 48 h, mainly in fibroblasts, cells of the vascular wall, and basal keratinocytes of migrating epithelium. In the control group, expressions of these proteins were low, which indicates that these Hsps are constitutive proteins in oral mucosa. Expression levels were similar to those reported in the healing of skin lesions and gastric ulcer, suggesting a common mechanism of Hsp activation in the repair of these tissues. (J Oral Sci 52, 623-631, 2010) Keywords: heat shock protein 27; heat shock protein 47; oral ulcer; skin ulcer; wound healing; immunohistochemistry.

Analysis of the expression of heat‐shock protein 27 in patients with oral lichen planus

“…A control group consisting of 10 biopsies of healthy oral mucosa (seven women, three no data available, with an average age of 37.86) was investigated for the expression of hsp27 . In this group, the following criteria of exclusion were chosen: (1) clinical signs of inflammation or presence of any visible lesion, (2) smoking and (3) wound healing areas, which have been related to an overexpression of HSPs (Leonardi et al , 2001, 2002; Hirano et al , 2004; Lee et al , 2008; Hacker et al , 2009; Pons et al , 2010; Vasques et al , 2010).…”

Immunohistochemical Changes of Heat Shock Protein 27 Expression in the Mouse Periodontal Tissues Exposed to Orthodontic Mechanical Stress

“…Furthermore, its expression has been observed in stellate reticulum in molar tooth germ, enamel organ and is involved in suppression of ameloblast proliferation and inhibition of apoptosis and has an important role in tooth formation 18) . It is involve in the repair of rat oral ulcers 19) and as a useful marker in squamous cell carcinoma differentiation 2) . HSP27 has been complicated by single gene, which is controlled by the constitutive expressions in tissues and cell types mostly in osteoblasts, muscle cells and epithelial cells 20) .…”