Immunolocalization of Inducible Nitric Oxide Synthase in Localized Juvenile Periodontitis Patients

Gašpirc, Boris; Masera, A; Skalerić, U

doi:10.1080/03008200290000628

Cited by 18 publications

(10 citation statements)

References 28 publications

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“…[19] Additionally, researchers in dentistry have evaluated the function of NO in inflammatory processes of oral mucosa,[20] periodontal tissues,[21–22] pulp[23] and periapical areas. [24] Although reactive intermediates of oxygen and nitrogen, such as NO, are frequently found at inflammatory and healing sites, their function in pulp and periapical tissues is not yet completely clear.…”

Section: Discussionmentioning

confidence: 99%

The use of aminoguanidine, a selective inducible nitric oxide synthase inhibitor, to evaluate the role of nitric oxide on periapical healing

Razavi¹,

Nejad²

2011

Dent Res J

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Background:Nitric oxide (NO) is one of the many chemical mediators involved in inflammatory processes. In addition to periapical inflammation, NO can have a role in periapical healing. The purpose of this study was to evaluate the effect of aminoguanidine (AG) as a selective inhibitor of inducible nitric oxide synthase (iNOS) on the degree of healing response of periapical lesions of the canine teeth of cats.Methods:In this interventional experimental study, the root canals of 48 cat canine teeth were infected with cat dental plaque and sealed. After induction of periapical lesions, root canal therapy (RCT) was performed. On the day of RCT phase, the cats were administered either AG (experimental group) or normal saline (control group), which was continued on a daily basis until the day of sacrifice. Four canine teeth in one cat served as negative and positive controls. The animals were sacrificed 6 weeks after RCT. The healing response of the periapical zones was analyzed histologically. The mean scores of healing for the two groups were compared using Mann–Whitney U test.Results:The mean scores of healing for the AG group (2.45±0.508) were significantly higher than those of the control group (2±0.510) (P<0.05).Conclusion:The use of an iNOS selective inhibitor such as AG can accelerate the healing process in periapical lesions.

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Section: Discussionmentioning

confidence: 99%

The use of aminoguanidine, a selective inducible nitric oxide synthase inhibitor, to evaluate the role of nitric oxide on periapical healing

Razavi¹,

Nejad²

2011

Dent Res J

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“…However, the extrapolation of the results of the present study in the immunopathogenesis of periodontal diseases needs to be investigated further. An increased number of iNOS + cells in the inflamed gingival tissues of humans (7)(8)(9)(10)(11) suggests that increased activity of iNOS may ameliorate the course of periodontal disease. Furthermore, our previous studies indicated that an increased Th1 response may be protective in periodontopathic-induced lesions in mice (28,29).…”

Section: Discussionmentioning

confidence: 99%

“…The immunopathogenesis of periodontal disease may be regulated by NO because an increased number of iNOS + cells have been observed in inflamed gingival tissue (7)(8)(9)(10)(11). In rodent models, the failure of iNOSdeficient and mercaptoethylguanidine-treated animals to develop Porphyromonas gingivalis-and ligature-induced alveolar bone loss, respectively, suggested that iNOS activity may be associated with destructive periodontal disease (12,13).…”

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confidence: 99%

Effect of inhibition of inducible nitric oxide synthase (iNOS) on the murine splenic immune response induced by Aggregatibacter (Actinobacillus) actinomycetemcomitans lipopolysaccharide

Sosroseno

Musa

Ravichandran

et al. 2008

European J Oral Sciences

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Animal studies suggest that inducible nitric oxide synthase (iNOS) may be associated with destructive periodontal disease. l-N(6)-(1-Iminoethyl)-lysine (L-NIL) has been shown to inhibit iNOS in a selective manner, and hence the aim of the present study was to test the hypothesis that treatment with l-NIL may induce a T-cell helper 1 (Th1)-like immune response by Aggregatibacter (Actinobacillus) actinomycetemcomitans lipopolysaccharide (LPS)-stimulated murine spleen cells in vitro. BALB/c mice were either sham-immunized or immunized with A. actinomycetemcomitans LPS. Spleen cells were stimulated with A. actinomycetemcomitans LPS in the presence or absence of L-NIL. Nitric oxide (NO), iNOS activity, specific IgG subclass antibodies, interferon-gamma (IFN-gamma), and interleukin-4 (IL-4) levels and cell proliferation were determined. The results showed that treatment with L-NIL suppressed both NO production and iNOS activity but enhanced specific IgG2a, IFN-gamma levels, and increased cell proliferation following stimulation with A. actinomycetemcomitans LPS-stimulated cells. The results of the present study suggest that inhibition of iNOS activity by L-NIL may skew the A. actinomycetemcomitans LPS-stimulated murine splenic immune response towards the Th1-like immune profile in vitro.

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“…In another study, it has been suggested that the expression of iNOS from macrophages in high levels may damage the periodontal tissues. iNOS activity in macrophages was reported to have the potential to inhibit leukocyte recruitment by acting on leukocytes that increase the inflammation in localized aggressive periodontitis (LAP) patients [22]. …”

Section: Introductionmentioning

confidence: 99%

Nitrite and Nitrate Levels of Gingival Crevicular Fluid and Saliva in Subjects with Gingivitis and Chronic Periodontitis

Ali¹,

Akalın²,

Sahbazoglu³

et al. 2014

JOMR

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ObjectivesNitrosative stress plays an essential role in the pathogenesis of periodontal disease. The aim of this study is to analyze the gingival crevicular fluid and saliva nitrite and nitrate levels in periodontally healthy and diseased sites.Material and MethodsA total of 60 individuals including, 20 chronic periodontitis and 20 gingivitis patients and 20 periodontally healthy controls participated in the present study. Probing depth, clinical attachment level, bleeding on probing, gingival index and plaque index were assessed, gingival crevicular fluid (GCF) and saliva samples were obtained from the subjects, including 480 GCF samples and 60 unstimulated whole saliva samples. Nitrite and nitrate were analyzed by Griess reagent.ResultsTotal GCF nitrite levels were higher in gingivitis and periodontitis groups (1.07 [SD 0.62] nmol and 1.08 [SD 0.59] nmol) than the control group (0.83 [SD 0.31] nmol) (P < 0.05) but did not differ significantly between gingivitis and periodontitis groups (P > 0.05). The difference in GCF nitrate level was not significant among the control, gingivitis and periodontitis groups (7.7 [SD 2.71] nmol, 7.51 [SD 4.16] nmol and 7.38 [SD 1.91] nmol). Saliva nitrite and nitrate levels did not differ significantly among three study groups. Saliva nitrate/nitrite ratios were higher in periodontitis and gingivitis groups than the control group. A gradual decrease in nitrate/nitrite ratio in GCF was detected with the presence of inflammation.ConclusionsIt may be suggested that nitrite in gingival crevicular fluid is a better periodontal disease marker than nitrate and may be used as an early detection marker of periodontal inflammation, and that local nitrosative stress markers don’t show significant difference between the initial and advanced stages of periodontal disease.

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Immunolocalization of Inducible Nitric Oxide Synthase in Localized Juvenile Periodontitis Patients

Cited by 18 publications

References 28 publications

The use of aminoguanidine, a selective inducible nitric oxide synthase inhibitor, to evaluate the role of nitric oxide on periapical healing

The use of aminoguanidine, a selective inducible nitric oxide synthase inhibitor, to evaluate the role of nitric oxide on periapical healing

Effect of inhibition of inducible nitric oxide synthase (iNOS) on the murine splenic immune response induced by Aggregatibacter (Actinobacillus) actinomycetemcomitans lipopolysaccharide

Nitrite and Nitrate Levels of Gingival Crevicular Fluid and Saliva in Subjects with Gingivitis and Chronic Periodontitis

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