Immunolocalization of Na+, K+-ATPase, Ca++-ATPase, Calcium-Binding Proteins, and Carbonic Anhydrase in the Guinea Pig Inner Ear

Ichimiya, Issei; Adams, Joe C.; Kimura, Robert S.

doi:10.3109/00016489409126037

Cited by 102 publications

(52 citation statements)

References 20 publications

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“…In the LW fraction, genes related to ion regulation and transport (8/23; 32%) and those involved in cellular development and proliferation (6/23; 24%) constitute the majority of differentially expressed genes. Both carbonic anhydrase genes (Car2 and Car14) and Na + , K + ATPase (Atp1b3) are all expressed in the lateral wall spiral ligament fibrocytes and are thought to play a role in cochlear fluid and ion homeostasis (Ichimiya et al 1994;Spicer et al 1997). K + homeostasis function of the marginal cells is mediated through the action of membrane proteins, such as Na + , K + ATPases, protein phosphatase inhibitors, and Na + , K + , Cl co-transporters (Zhao et al 1994;Agrup et al 1997;Spicer et al 1997;Delpire et al 1999;Dixon et al 1999;Wangemann 2002).…”

Section: Discussionmentioning

confidence: 99%

Differential Expression of Genes within the Cochlea as Defined by a Custom Mouse Inner Ear Microarray

Morris

Snir²,

Pompéia

et al. 2005

JARO

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Microarray analyses have contributed greatly to the rapid understanding of functional genomics through the identification of gene networks as well as gene discovery. To facilitate functional genomics of the inner ear, we have developed a mouse inner-earpertinent custom microarray chip (CMA-IE1). Nonredundant cDNA clones were obtained from two cDNA library resources: the RIKEN subtracted inner ear set and the NIH organ of Corti library. At least 2000 cDNAs unique to the inner ear were present on the chip. Comparisons were performed to examine the relative expression levels of these unique cDNAs within the organ of Corti, lateral wall, and spiral ganglion. Total RNA samples were obtained from the three cochlear-dissected fractions from adult CF-1 mice. The total RNA was linearly amplified, and a dendrimer-based system was utilized to enhance the hybridization signal. Differentially expressed genes were verified by comparison to known gene expression patterns in the cochlea or by correlation with genes and gene families deduced to be present in the three tissue types. Approximately 22Y25% of the genes on the array had significant levels of expression. A number of differentially expressed genes were detected in each tissue fraction. These included genes with known functional roles, hypothetical genes, and various unknown or uncharacterized genes. Four of the differentially expressed genes found in the organ of Corti are linked to deafness loci. None of these are hypothetical or unknown genes.

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Section: Discussionmentioning

confidence: 99%

Differential Expression of Genes within the Cochlea as Defined by a Custom Mouse Inner Ear Microarray

Morris

Snir²,

Pompéia

et al. 2005

JARO

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“…It is known that there are two general classes of ATP-driven Ca ++ pumps; they are present in the smooth endoplasmic reticulum and in the plasma membrane (Blaustein 1988). It has been reported that both cytosolic (Schulte 1993;Ichimiya et al 1994a) and plasma membrane Ca ++ -ATPase (Yoshihara and Igarashi 1987;Crouch and Schulte 1995;Apicella et al 1997;Curtis et al 1997) are present in inner ear tissue where they regulate calcium-sensitive cellular processes. In the present study, the localization of three different plasma membrane Ca ++ -ATPases, as well as the Na + ,Ca ++ exchanger, Na + ,K + -ATPase, CKBB, and CA was demonstrated.…”

Section: Cytochemical Effects Of Gentamicin Treatmentmentioning

confidence: 99%

“…There are a number of morphologically distinctive cell types within the ligament, the most abundant of which is the type I fibrocyte. Type I fibrocytes have been found to show marked cytochemical changes that progressed with development of endolymphatic hydrops in the guinea pig hydrops model (Ichimiya et al 1994a), a finding that strongly suggested that these cells play an essential role in control of cochlear fluid volume. All cells within the ligament are interconnected via gap junctions, one consequence of which is that it makes possible the recycling of potassium ions from the organ of Corti to the stria vascularis (Kikuchi et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Changes in Cytochemistry of Sensory and Nonsensory Cells in Gentamicin-Treated Cochleas

Imamura

Adams

2003

JARO - Journal of the Association for Research in Otolaryngolog

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Effects of a single local dose of gentamicin upon sensory and nonsensory cells throughout the cochlea were assessed by changes in immunostaining patterns for a broad array of functionally important proteins. Cytochemical changes in hair cells, spiral ganglion cells, and cells of the stria vascularis, spiral ligament, and spiral limbus were found beginning 4 days post administration. The extent of changes in immunostaining varied with survival time and with cell type and was not always commensurate with the degree to which individual cell types accumulated gentamicin. Outer hair cells, types I and II fibrocytes of the spiral ligament, and fibrocytes in the spiral limbus showed marked decreases in immunostaining for a number of constituents. In contrast, inner hair cells, type III fibrocytes and root cells of the spiral ligament, cells of the stria vascularis, and interdental cells in the spiral limbus showed less dramatic decreases, and in some cases they showed increases in immunostaining. Results indicate that, in addition to damaging sensory cells, local application of gentamicin results in widespread and disparate disruptions of a variety of cochlear cell types. Only in the case of ganglion cells was it apparent that the changes in nonsensory cells were secondary to loss or damage of hair cells. These results indicate that malfunction of the ear following gentamicin treatment is widespread and far more complex than simple loss of sensory elements. The results have implications for efforts directed toward detecting, preventing, and treating toxic effects of aminoglycosides upon the inner ear.

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“…The rationale for the current study comes from the finding that the cochlea contains a number of proteins known to interact with NHERFs, plus numerous other proteins that have PDZ domains that could potentially interact with NHERFs. These cochlear proteins include NHE3 (Bond et al 1998), the ERM family (Kitajiri et al 2004), actin (Geal-Dor et al 1993;Nishida et al 1998), myosin (Boeda et al 2002;Johnson et al 2003;Mburu et al 2003;Kitajiri et al 2004;Belyantseva et al 2005;Kikkawa et al 2005), NBC3 (Bok et al 2003), P2Y1 (Teixeira et al 2000), β-adrenergic receptors (Fauser et al 2004), the vacuolar proton pump v-H + -ATPase (Stankovic et al 1997), Na + -K + ATPase (Ichimiya et al 1994;Erichsen et al 1996), growth factor receptors (Pickles and van Heumen 1997), glucocorticoid receptors (Erichsen et al 1996), TASK-1 background K + channels (Kanjhan et al 2004a), inwardly rectifying K + channels Kir1.1 (ROMK1) (Glowatzki et al 1995), Kir4.1 and Kir5.1 (Hibino et al 2004), aquaporin-4 (Mhatre et al 2002), glutamate transporter GLAST (Furness and Lehre 1997), and plasma membrane Ca 2+ -ATPase (Ichimiya et al 1994;Dumont et al 2001). …”

Section: Discussionmentioning

confidence: 99%

Postnatal developmental expression of the PDZ scaffolds Na+-H+ exchanger regulatory factors 1 and 2 in the rat cochlea

et al. 2005

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Sensory transduction in the mammalian cochlea requires the maintenance of specialized fluid compartments with distinct ionic compositions. This is achieved by the concerted action of diverse ion channels and transporters, some of which can interact with the PDZ scaffolds, Na + -H + exchanger regulatory factors 1 and 2 (NHERF-1, NHERF-2). Here, we report that NHERF-1 and NHERF-2 are widely expressed in the rat cochlea, and that their expression is developmentally regulated. Reverse transcription/polymerase chain reaction (RT-PCR) and Western blotting initially confirmed the RNA and protein expression of NHERFs. We then performed immunohistochemistry on cochlea during various stages of postnatal development. Prior to the onset of hearing (P8), NHERF-1 immunolabeling was prominently polarized to the apical membrane of cells lining the endolymphatic compartment, including the stereocilia and cuticular plates of the inner and outer hair cells, marginal cells of the stria vascularis, Reissner's epithelia, and tectorial membrane. With maturation (P21, P70), NHERF-1 immunolabeling was reduced in the above structures, whereas labeling increased in the apical membrane of the interdental cells of the spiral limbus and the inner and outer sulcus cells, Hensen's cells, the inner and outer pillar cells, Deiters cells, the inner border cells, spiral ligament fibrocytes, and spiral ganglion neurons (particularly type II). NHERF-1 expression in strial basal and intermediate cells was persistent. NHERF-2 immunolabeling was similar to that for NHERF-1 during postnatal development, with the exception of expression in the synaptic regions beneath the outer hair cells. NHERF-1 and NHERF-2 co-localized with glial fibrillary acidic protein and vimentin in glia. The cochlear localization of NHERF scaffolds suggests that they play important roles in the developmental regulation of ion transport, homeostasis, and auditory neurotransmission.

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Immunolocalization of Na⁺, K⁺-ATPase, Ca⁺⁺-ATPase, Calcium-Binding Proteins, and Carbonic Anhydrase in the Guinea Pig Inner Ear

Cited by 102 publications

References 20 publications

Differential Expression of Genes within the Cochlea as Defined by a Custom Mouse Inner Ear Microarray

Differential Expression of Genes within the Cochlea as Defined by a Custom Mouse Inner Ear Microarray

Changes in Cytochemistry of Sensory and Nonsensory Cells in Gentamicin-Treated Cochleas

Postnatal developmental expression of the PDZ scaffolds Na+-H+ exchanger regulatory factors 1 and 2 in the rat cochlea

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