Immunolocalization of Sphingolipid Catabolism Enzymes along the Nephron: Novel Early Urinary Biomarkers of Renal Damage

Franco, Martha; Cano-Martínez, Agustina; Ramos-Godínez, María del Pilar; López-Marure, Rebeca; Donis-Maturano, Luis; Sosa, José Santamaría; Bautista-Pérez, Rocio

doi:10.3390/ijms242316633

Cited by 2 publications

(1 citation statement)

References 62 publications

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“…A series of elegant basic studies recently suggested that sphingolipids are promising urinary biomarkers for kidney diseases ( 1 , 2 ). The sphingolipids are composed of a hydrophobic and a hydrophilic chain, with the various fatty acid components of each sphingolipid determining its species ( 3 ).…”

Section: Introductionmentioning

confidence: 99%

Alterations in urinary ceramides, sphingoid bases, and their phosphates among patients with kidney disease

Morita,

Sakai,

Isago

et al. 2024

Front. Nephrol.

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BackgroundTo avoid an invasive renal biopsy, noninvasive laboratory testing for the differential diagnosis of kidney diseases is a desirable goal. As sphingolipids are demonstrated to be involved in the pathogenesis of various kidney diseases, we investigated the possible usefulness of the simultaneous measurement of urinary sphingolipids for differentiating kidney diseases.Materials and methodsResidual urine specimens were collected from patients who had been clinically diagnosed with chronic glomerulonephritis (CGN), diabetic mellitus (DM), systemic lupus erythematosus (SLE), and arterial hypertension (AH). The urinary sphingolipids—CERs C16:0, C18:0, C18:1, C20:0, C22:0, and C24:0; sphingosine [Sph]; dihydrosphingosine; sphingosine 1-phosphate [S1P]; and dihydroS1P [dhS1P]—were measured by liquid chromatography–tandem mass spectrometry. Based on the results, machine learning models were constructed to differentiate the various kidney diseases.ResultsThe urinary S1P was higher in patients with DM than in other participants (P < 0.05), whereas dhS1P was lower in the CGN and AH groups compared with control participants (P < 0.05). Sph and dhSph were higher in patients with CGN, AH, and SLE than in those with control participants (P < 0.05). The urinary CERs were significantly higher in patients with CGN, AH, and SLE than in those with control participants (P < 0.05). As a results of constructing a machine learning model discriminating kidney diseases, the resulting diagnostic accuracy and precision were improved from 94.03% and 66.96% to 96.10% and 78.26% respectively, when the urinary CERs, Sph, dhSph, S1P, dhS1P, and their ratios were added to the models.ConclusionThe urinary CERs, sphingoid bases, and their phosphates show alterations among kidney diseases, suggesting their potential involvement in the development of kidney injury.

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Section: Introductionmentioning

confidence: 99%

Alterations in urinary ceramides, sphingoid bases, and their phosphates among patients with kidney disease

Morita,

Sakai,

Isago

et al. 2024

Front. Nephrol.

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Glycolipid Metabolic Disorders, Metainflammation, Oxidative Stress, and Cardiovascular Diseases: Unraveling Pathways

de Lima,

Moretti,

Torres Pomini

et al. 2024

Biology

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Glycolipid metabolic disorders (GLMDs) are various metabolic disorders resulting from dysregulation in glycolipid levels, consequently leading to an increased risk of obesity, diabetes, liver dysfunction, neuromuscular complications, and cardiorenal vascular diseases (CRVDs). In patients with GLMDs, excess caloric intake and a lack of physical activity may contribute to oxidative stress (OxS) and systemic inflammation. This study aimed to review the connection between GLMD, OxS, metainflammation, and the onset of CRVD. GLMD is due to various metabolic disorders causing dysfunction in the synthesis, breakdown, and absorption of glucose and lipids in the body, resulting in excessive ectopic accumulation of these molecules. This is mainly due to neuroendocrine dysregulation, insulin resistance, OxS, and metainflammation. In GLMD, many inflammatory markers and defense cells play a vital role in related tissues and organs, such as blood vessels, pancreatic islets, the liver, muscle, the kidneys, and adipocytes, promoting inflammatory lesions that affect various interconnected organs through their signaling pathways. Advanced glycation end products, ATP-binding cassette transporter 1, Glucagon-like peptide-1, Toll-like receptor-4, and sphingosine-1-phosphate (S1P) play a crucial role in GLMD since they are related to glucolipid metabolism. The consequences of this is system organ damage and increased morbidity and mortality.

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Immunolocalization of Sphingolipid Catabolism Enzymes along the Nephron: Novel Early Urinary Biomarkers of Renal Damage

Cited by 2 publications

References 62 publications

Alterations in urinary ceramides, sphingoid bases, and their phosphates among patients with kidney disease

Alterations in urinary ceramides, sphingoid bases, and their phosphates among patients with kidney disease

Glycolipid Metabolic Disorders, Metainflammation, Oxidative Stress, and Cardiovascular Diseases: Unraveling Pathways

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