Immunolocalization of the Cholesterol Transporters <scp>ABCA</scp>1 and <scp>ABCG</scp>1 in Canine Reproductive Tract Tissues and Spermatozoa

Palme, N; Becher, AC; Merkl, M.; Glösmann, Martin; Aurich, Christine; Schäfer-Somi, Sabine

doi:10.1111/rda.12294

Cited by 18 publications

(26 citation statements)

References 28 publications

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“…Cholesterol efflux in sperm has been shown to be dependent upon active externalization of the sterol to make it available to sterol acceptors in the medium; this takes place through members of the ATP-binding cassette (ABC) transporter superfamily in mice (Morales et al 2012). ABCA1, ABCA7, ABCA17, and ABCG1 have been detected in mouse sperm (Morales et al 2008) and ABCA1 has also been identified in dog sperm (Palme et al 2014). Inhibition of ABCA1, ABCA7, and ABCG1 in mouse sperm in turn inhibits IVF, confirming that cholesterol efflux plays a core role in the physiology of capacitation and gamete interaction.…”

Section: Introductionmentioning

confidence: 69%

Effect of calcium, bicarbonate, and albumin on capacitation-related events in equine sperm

Macías‐García¹,

González‐Fernández²,

Loux³

et al. 2015

Reproduction

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Repeatable methods for IVF have not been established in the horse, reflecting the failure of standard capacitating media to induce changes required for fertilization capacity in equine sperm. One important step in capacitation is membrane cholesterol efflux, which in other species is triggered by cholesterol oxidation and is typically enhanced using albumin as a sterol acceptor. We incubated equine sperm in the presence of calcium, BSA, and bicarbonate, alone or in combination. Bicarbonate induced an increase in reactive oxygen species (ROS) that was abolished by the addition of calcium or BSA. Bicarbonate induced protein tyrosine phosphorylation (PY), even in the presence of calcium or BSA. Incubation at high pH enhanced PY but did not increase ROS production. Notably, no combination of these factors was associated with significant cholesterol efflux, as assessed by fluorescent quantitative cholesterol assay and confirmed by filipin staining. By contrast, sperm treated with methyl-b-cyclodextrin showed a significant reduction in cholesterol levels, but no significant increase in PY or ROS. Presence of BSA increased sperm binding to bovine zonae pellucidae in all three stallions. These results show that presence of serum albumin is not associated with a reduction in membrane cholesterol levels in equine sperm, highlighting the failure of equine sperm to exhibit core capacitation-related changes in a standard capacitating medium. These data indicate an atypical relationship among cholesterol efflux, ROS production, and PY in equine sperm. Our findings may help to elucidate factors affecting failure of equine IVF under standard conditions.

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Section: Introductionmentioning

confidence: 69%

Effect of calcium, bicarbonate, and albumin on capacitation-related events in equine sperm

Macías‐García¹,

González‐Fernández²,

Loux³

et al. 2015

Reproduction

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“…OCTN2 has also been characterized for its ability to transport carnitine, an important nutrient for male fertility, in MGT tissues [51]. Several ABC transporters have also been implicated in cholesterol transport within the testis, spermatozoa, and epididymis [52–55]. …”

Section: Discussionmentioning

confidence: 99%

Xenobiotic transporter expression along the male genital tract

Klein

Wright

Cherrington

2014

Reproductive Toxicology

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The male genital tract plays an important role in protecting sperm by forming a distinct compartment separate from the body which limits exposure to potentially toxic substrates. Transporters along this tract can influence the distribution of xenobiotics into the male genital tract through efflux back into the blood or facilitating the accumulation of toxicants. The aim of this study was to quantitatively determine the constitutive mRNA expression of 30 xenobiotic transporters in caput and cauda regions of the epididymis, vas deferens, prostate, and seminal vesicles from adult Sprague-Dawley rats. The epididymis was found to express at least moderate levels of 18 transporters, vas deferens 15, seminal vesicles 23, and prostate 18. Constitutive expression of these xenobiotic transporters in the male genital tract may provide insight into the xenobiotics that can potentially be transported into these tissues and may provide the molecular mechanism for site specific toxicity of select agents.

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“…ABC transporters are a protein superfamily characterized by the presence of ATP-binding cassette (ABC) domains that are involved in inward and outward transport of a wide variety of substrates such as metabolites, drugs, and lipids. In particular, our analysis demonstrates that the ABC transporters play a key role in epididymal biochemistry of sperm membrane rearrangement [Ouvrier et al 2009;Jones and Cry 2011;Morales et al 2012;Caballero et al 2012;Gregory and Cyr 2014;Palme et al 2014]. They are a superfamily of more than 250 transmembrane proteins characterized by a region that spans about 180 amino acids and contains three highly conserved motifs: the Walker A/P-loop (12 amino acids), a signature motif/ C-loop (5 amino acids), and the Walker B motif (5 amino acids) [George and Jones 2012].…”

Section: Node Degreementioning

confidence: 65%

The maturation of murine spermatozoa membranes within the epididymis, a computational biology perspective

Bernabò

Agostino

Ordinelli

et al. 2016

Systems Biology in Reproductive Medicine

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(2016) The maturation of murine spermatozoa membranes within the epididymis, a computational biology perspective, Systems Biology in Reproductive Medicine, 62:5, 299-308, DOI: 10.1080/19396368.2016 To link to this article: https://doi.org/10. 1080/19396368.2016.1205679 To become fertile, mammalian spermatozoa require completing a complex biochemical maturation that begins in the testis and ends within the female oviduct. Here, we paid attention to the events occurring at the membrane level during the epididymal transit. Indeed, in the epididymis, the molecular composition and the physical-chemical proprieties of sperm membranes markedly change, with functional cross talking among the spermatozoa, the epithelium, and the luminal content (particularly the epididymosomes). To study this process, we undertook a biological networks study, representing the involved molecules as nodes and their interactions as links. The analysis of network topology revealed that it has a scale free and small world architecture and it is robust against random failure. That assures a fast and efficient transmission of information and it leads to identifying the molecules exerting a higher level of control on the system, among which cholesterol plays a pivotal role. The reactome enrichment analysis allowed the reconstruction of the biochemical pathways involved in sperm epididymal maturation and STRING analysis permitted the identification of molecular events possibly involved in that process. In conclusion, this approach allows inferring interesting information, thus contributing to the knowledge on this process and suggesting staring points for further research. ARTICLE HISTORY

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Immunolocalization of the Cholesterol Transporters ABCA1 and ABCG1 in Canine Reproductive Tract Tissues and Spermatozoa

Cited by 18 publications

References 28 publications

Effect of calcium, bicarbonate, and albumin on capacitation-related events in equine sperm

Effect of calcium, bicarbonate, and albumin on capacitation-related events in equine sperm

Xenobiotic transporter expression along the male genital tract

The maturation of murine spermatozoa membranes within the epididymis, a computational biology perspective

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