Immunolocalization of transforming growth factor-betas and their receptors in the intervertebral disk of senescence-accelerated mouse.

Nagano, Satoshi; Matsunaga, Shunji; Takae, Reiko; Morimoto, Norio; Suzuki, Shintaro; Yoshida, Hiroki

doi:10.3892/ijo.17.3.461

Cited by 5 publications

(6 citation statements)

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“…TGF-β1, BMP-2, and BMP-7, are upregulated in both nucleus pulposus and annulus fibrosus [9]. This is in contrast to previous studies on senescence-accelerated mice where a decline of all three TGF-β isoforms and their receptors (TbetaR-I and TbetaR-II) was observed [40,41]. These differences maybe due to the different species tested or to the technique used (immunohistochemistry versus polymerase chain reaction).…”

Section: Introductioncontrasting

confidence: 83%

Growth Factors in Intervertebral Disc Homeostasis

Pratsinis¹,

Kletsas²

2008

Connective Tissue Research

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Intervertebral disc degeneration is a complex age-related pathology associated with back pain. Research on the growth factors that regulate disc homeostasis is of critical importance for understanding the basis of the disease. Here we summarize the data on the expression and function of various growth factors in the disc from in vivo and in vitro studies, as well as on their alterations during degeneration and ageing. Such studies are becoming more crucial in the prospect of clinical application of growth factors for the treatment of disc degeneration.

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Section: Introductioncontrasting

confidence: 83%

Growth Factors in Intervertebral Disc Homeostasis

Pratsinis¹,

Kletsas²

2008

Connective Tissue Research

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“…Even though we consider ICR mice to be valid controls for SAMP1 mice (Lee et al 2013; Nagano et al 2000; Sakakima et al 2004), we cannot rule out the possibility that the observed differences in the results obtained with ICR and SAMP1 mice may be due to differences between the strains. Because Derave et al (2005) stated that SAMR1 mice were not suitable because of difficulties in breeding them, and because we have had problems with tumors of the oral cavity in SAMR1 mice, we prefer to use ICR mice as controls for SAMP1 mice in our studies.…”

Section: Discussionmentioning

confidence: 94%

“…ICR mice are widely used as a control strain in experiments on SAMP1 (Lee et al 2013; Nagano et al 2000). Male 15-week-old SAMP1 and ICR mice were purchased from Japan SLC, Inc. (Hamamatsu, Japan) and maintained under controlled conditions of temperature (23 ± 2°C), humidity (55% ± 10%), and lighting (0700 to 1900 h).…”

Section: Methodsmentioning

confidence: 99%

Habitual exercise plus dietary supplementation with milk fat globule membrane improves muscle function deficits via neuromuscular development in senescence-accelerated mice

et al. 2014

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We examined the effects of habitual exercise plus nutritional intervention through consumption of milk fat globule membrane (MFGM), a milk component, on aging-related deficits in muscle mass and function in senescence-accelerated P1 mice. Combining wheel-running and MFGM (MFGMEx) intake significantly attenuated age-related declines in quadriceps muscle mass (control: 318 ± 6 mg; MFGMEx: 356 ± 9 mg; P < 0.05) and in contractile force (1.4-fold and 1.5-fold higher in the soleus and extensor digitorum longus muscles, respectively). Microarray analysis of genes in the quadriceps muscle revealed that MFGMEx stimulated neuromuscular development; this was supported by significantly increased docking protein-7 (Dok-7) and myogenin mRNA expression. Treatment of differentiating myoblasts with MFGM-derived phospholipid or sphingolipid fractions plus mechanical stretching also significantly increased Dok-7 mRNA expression. These findings suggest that habitual exercise plus dietary MFGM improves muscle function deficits through neuromuscular development, and that phospholipid and sphingolipid in MFGM contribute to its physiological actions.Electronic supplementary materialThe online version of this article (doi:10.1186/2193-1801-3-339) contains supplementary material, which is available to authorized users.

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“…Interestingly, the results from different research groups exhibit different and even opposite changes in the expression of TGF-b signaling in IVD. In a senescence-accelerated mouse model, immunohistochemical staining results showed that the expressions of TGF-bs and TGF-b receptors decreased with age in IVDs 37,38 . And it was also reported that the expression of pSMAD2/3 in the IVDs was significantly decreased in old mice (18 months) relative to young mice (2 months) 39 .…”

Section: The Changes In the Expression Of Tgf-b Signaling In Ivdmentioning

confidence: 99%

TGF-β signaling in intervertebral disc health and disease

Chen

Liu

et al. 2019

Osteoarthritis and Cartilage

104

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Objective: This paper aims to provide a comprehensive review of the changing role of transforming growth factor-b (TGF-b) signaling in intervertebral disc (IVD) health and disease. Methods: A comprehensive literature search was performed using PubMed terms 'TGF-b' and 'IVD'. Results: TGF-b signaling is necessary for the development and growth of IVD, and can play a protective role in the restoration of IVD tissues by stimulating matrix synthesis, inhibiting matrix catabolism, inflammatory response and cell loss. However, excessive activation of TGF-b signaling is detrimental to the IVD, and inhibition of the aberrant TGF-b signaling can delay IVD degeneration. Conclusions: Activation of TGF-b signaling has a promising treatment prospect for IVD degeneration, while excessive activation of TGF-b signaling may contribute to the progression of IVD degeneration. Studies aimed at elucidating the changing role of TGF-b signaling in IVD at different pathophysiological stages and its specific molecular mechanisms are needed, and these studies will contribute to safe and effective TGF-b signaling-based treatments for IVD degeneration.

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Immunolocalization of transforming growth factor-betas and their receptors in the intervertebral disk of senescence-accelerated mouse.

Cited by 5 publications

References 0 publications

Growth Factors in Intervertebral Disc Homeostasis

Growth Factors in Intervertebral Disc Homeostasis

Habitual exercise plus dietary supplementation with milk fat globule membrane improves muscle function deficits via neuromuscular development in senescence-accelerated mice

TGF-β signaling in intervertebral disc health and disease

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