Immunologic reaction and viability of cryopreserved homografts

Fischlein, T; Schütz, Albert J.; Haushofer, M; Frey, R; Uhlig, A; Detter, Christian; Reichart, Bruno

doi:10.1016/0003-4975(95)00257-l

Cited by 53 publications

(27 citation statements)

References 7 publications

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“…In other studies that detected a high risk for late tissue degeneration in nonviable grafts, it remains unclear whether the need for reoperation is due to a nonviable graft at implantation or to structural deterioration because of suboptimal preservation techniques such as freeze-drying [Daly et al, 1991]. In addition, conventionally frozen cryopreserved allograft heart valves elicit an immunological reaction within the recipient [Fischlein et al, 1995]. It is still not clear how these initial inflammatory reactions affect the long-term performance of allografts, but since viable cells may trigger an increased immune response, the lower viability of the transplant may be beneficial [Muratov et al, 2010].…”

Section: Discussionmentioning

confidence: 99%

Preclinical Evaluation of Ice-Free Cryopreserved Arteries: Structural Integrity and Hemocompatibility

Huber¹,

Brockbank²,

Riemann

et al. 2012

Cells Tissues Organs

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Objective: Arterial allografts are routinely employed for reconstruction of infected prosthetic grafts. Usually, banked cryopreserved arteries are used; however, existing conventional freezing cryopreservation techniques applied to arteries are expensive. In contrast, a new ice-free cryopreservation technique results in processing, storage and shipping methods that are technically simpler and potentially less costly. The objective of this study was to determine whether or not ice-free cryopreservation causes tissue changes that might preclude clinical use. Methods: Conventionally frozen cryopreserved porcine arteries were compared with ice-free cryopreserved arteries and untreated fresh controls using morphological (light, scanning electron and laser scanning microscopy), viability (alamarBlue assay) and hemocompatibility methods (blood cell adhesion, thrombin/antithrombin-III-complex, polymorphonuclear neutrophil-elastase, β-thromboglobulin and terminal complement complex SC5b-9). Results: No statistically significant structural or hemocompatibility differences between ice-free cryopreserved and frozen tissues were detectable. There were no quantitative differences observed for either autofluorescence (elastin) or second harmonic generation (collagen) measured by laser scanning microscopy. Cell viability in ice-free cryopreserved arteries was significantly reduced compared to fresh and frozen tissues (p < 0.05). Conclusions: The formation of ice in aortic artery preservation did not make a difference in histology, structure or thrombogenicity, but significantly increased viability compared with a preservation method that precludes ice formation. Reduced cell viability should not reduce in vivo performance. Therefore, ice-free cryopreservation is a potentially safe and cost-effective technique for the cryopreservation of blood vessel allografts.

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Section: Discussionmentioning

confidence: 99%

Preclinical Evaluation of Ice-Free Cryopreserved Arteries: Structural Integrity and Hemocompatibility

Huber¹,

Brockbank²,

Riemann

et al. 2012

Cells Tissues Organs

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“…So far, cryopreserved aortic homograft showed its potential for in situ repair. 7,10,11,19) Durability of such repairs remains to be confirmed on the ground of higher patient volume with extended follow-up. Different methods for visceral debranching as well as optimal duration of preoperative and postoperative antibiotic treatment are still discussed.…”

Section: Resultsmentioning

confidence: 99%

“…The favorable long-term results of implanted cryopreserved human cardiac valves 17) have propelled the more common use of cryopreserved aortic homografts within the setting of aortic infections. 7,10,11) There is evidence which indicates that cryopreserved homografts are resistant to bacterial infection, 18,19) and that the immune response of the host may be limited. 19) In our patient wide debridement of the infected and necrotic tissue was performed prior to graft placement.…”

Section: Discussionmentioning

confidence: 99%

“…7,10,11) There is evidence which indicates that cryopreserved homografts are resistant to bacterial infection, 18,19) and that the immune response of the host may be limited. 19) In our patient wide debridement of the infected and necrotic tissue was performed prior to graft placement. The cryopreserved aortic homograft was then positioned within the most critical infected area next to the affected vertebral bodies and involved intervertebral disc.…”

Section: Discussionmentioning

confidence: 99%

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In Situ Repair of a Mycotic Suprarenal Aortic Aneurysm Using a Cryopreserved Aortic Homograft and Visceral Debranching in a Patient with Spondylodiscitis and Left Psoas Muscle Abscess

Petrunić

Meštrović

Lončarić

et al. 2013

ATCS

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We present a patient with ruptured suprarenal aortic aneurysm, involving origins of visceral and renal arteries. Associated spondylodiscitis and left psoas muscle abscess were also diagnosed. The patient was initially treated with antibiotics. Diagnostic survey showed progression of the aneurysm diameter and enlargement of the psoas muscle abscess. Surgical treatment using a cryopreserved aortic homograft with debranching of visceral arteries was performed. Different modalities of surgical repair within the infected aortic segment and the rationale for usage of cryopreserved homografts are considered. The importance of optimal timing for surgery is emphasized as well.

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“…It has been suggested that the possible factors that influence the long-term durability of cardiac valves are immunological compatibility and cellular viability (Angell 1987;Fischlein et al 1995). Nevertheless the impact of allograft valve viability on valve durability remains controversial.…”

Section: Introductionmentioning

confidence: 98%

Apoptosis in fresh and cryopreserved cardiac valves of pig samples

Vázquez

Roman

et al. 2008

Cell Tissue Banking

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To analyse the influence of cold ischemic time (CIT) (2-24 h) and of cryopreservation (liquid phase) on the viability of the valvular fibroblasts and in the presence of apoptosis. Cardiac valves from 10 pigs were evaluated by anatomo-pathological study of the wall, muscle and leaflet. At the same time, the presence of cellular death due to apoptosis was investigated in two ways; directly on tissue by Apodetec system and by two-colour flow cytometry assay analyzing a suspension of fibroblast from valve leaflets using Anexina V and propidium iodure (PI). We established three groups of samples to compare different experimental conditions: 2 h of ischemia (group 1), 24 h of ischemia (group 2), and a programme of cryopreservation (-1 degrees C/min) after 2 h of ischemia, followed by storage in liquid nitrogen during a week and thawing was performed (group 3). The analysis of viabilities showed slight differences between all three groups. The results indicated CIT of 24 h undergoing more structural affectation than CIT of 2 h. Flow cytometry analysis did not show important differences between groups; however cryopreserved samples (group 3) slightly less viability and a higher percentage of death by apoptosis than group 1 and 2 using flow cytometry. Apoptosis was confirmed on tissue from all valves but mainly in samples of group 2 and group 3. In summary, the viability of the valves in the case of ischemic times of 2 h, 24 h or after cryopreservation/thawing differs slightly. The death of the cells is mainly mediated by necrosis and not by apoptosis.

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Immunologic reaction and viability of cryopreserved homografts

Cited by 53 publications

References 7 publications

Preclinical Evaluation of Ice-Free Cryopreserved Arteries: Structural Integrity and Hemocompatibility

Preclinical Evaluation of Ice-Free Cryopreserved Arteries: Structural Integrity and Hemocompatibility

In Situ Repair of a Mycotic Suprarenal Aortic Aneurysm Using a Cryopreserved Aortic Homograft and Visceral Debranching in a Patient with Spondylodiscitis and Left Psoas Muscle Abscess

Apoptosis in fresh and cryopreserved cardiac valves of pig samples

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