Immunologic Studies with Malleomyces Mallei and Malleomyces Pseudomallei. I. Serological Relationships between M. Mallei and M. Pseudomallei

Cravitz, Leo; Miller, Winston R.

doi:10.1093/infdis/86.1.46

Cited by 32 publications

(22 citation statements)

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“…and Bacillus mallei Our characterization of Pseudomonas pseudomallei and Bacillus mallei in terms of physiological and nutritional properties amply confirms the close resemblance between these two species, which has been suggested by others on the basis of simi-larities in pathogenicity (Whitmore, 1913), antigenic constitution (Stanton & Fletcher, 1925;Cravitz & Miller, 1950) and phage susceptibility (Smith & Cherry, 1957). The phenotypic resemblances assume even deeper significance in view of the virtual identity in the GC content of the respective DNAs (Mandel, 1966).…”

Section: The Afinities and Taxonomic Position Of Pseudomonas Pseudomasupporting

confidence: 79%

“…B. pseudomallei was discovered and described by Whitmore (1913), and his choice of a specific name was determined by its resemblances in pathological and cultural respects to the agent of glanders. Subsequently, Stanton & Fletcher (1925) and Cravitz & Miller (1950) showed that there are also serological relationships between these two bacterial species. For several decades the systematic position of B. pseudomallei also remained obscure, and it followed B. mallei through Malleorn yces, Loeflerella and Pfeifferella.…”

Section: Introductionmentioning

confidence: 99%

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A Comparative Study of Pseudomonas pseudomallei and Bacillus mallei

Redfearn

Palleroni

Stanier

1966

Journal of General Microbiology

108

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SUMMARYA comparative study of many strains of Pseudom,onas pseudomallei and Bacillus mallei has shown that these two species are very similar with respect to their nutritional and biochemical properties, thus confirming earlier claims of a relationship between them, based on such criteria as pathological and serological properties. P. pseudomallei is in all respects a typical and nutritionally highly versatile member of the genus Pseudomonas. In view of this fact we propose that B. mallei should also be placed in the genus Pseudomonas, even though it is a permanently non-motile bacterium. The ecology and possible evolutionary relationships between the two species are discussed in the light of the present findings.

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Section: The Afinities and Taxonomic Position Of Pseudomonas Pseudomasupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Pseudomonas pseudomallei and Bacillus mallei

Redfearn

Palleroni

Stanier

1966

Journal of General Microbiology

108

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“…As the names suggest, B. mallei and B. pseudomallei are closely related species (19,56,59,69). These ␤-Proteobacteria can now be directly compared at the genomic level because the B. pseudomallei K96243 genomic sequence is available at the Sanger Institute website (http://www.sanger.ac.uk/) and the B. mallei ATCC 23344 genomic sequence is available at the TIGR (The Institute for Genomic Research) website (http://www.tigr.org/).…”

mentioning

confidence: 99%

Burkholderia thailandensis E125 Harbors a Temperate Bacteriophage Specific for Burkholderia mallei

et al. 2002

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Burkholderia thailandensis is a nonpathogenic gram-negative bacillus that is closely related to Burkholderia mallei and Burkholderia pseudomallei. We found that B. thailandensis E125 spontaneously produced a bacteriophage, termed E125, which formed turbid plaques in top agar containing B. mallei ATCC 23344. We examined the host range of E125 and found that it formed plaques on B. mallei but not on any other bacterial species tested, including B. thailandensis and B. pseudomallei. Examination of the bacteriophage by transmission electron microscopy revealed an isometric head and a long noncontractile tail. B. mallei NCTC 120 and B. mallei DB110795 were resistant to infection with E125 and did not produce lipopolysaccharide (LPS) O antigen due to IS407A insertions in wbiE and wbiG, respectively. wbiE was provided in trans on a broad-hostrange plasmid to B. mallei NCTC 120, and it restored LPS O-antigen production and susceptibility to E125. The 53,373-bp E125 genome contained 70 genes, an IS3 family insertion sequence (ISBt3), and an attachment site (attP) encompassing the 3 end of a proline tRNA (UGG) gene. While the overall genetic organization of the E125 genome was similar to -like bacteriophages and prophages, it also possessed a novel cluster of putative replication and lysogeny genes. The E125 genome encoded an adenine and a cytosine methyltransferase, and purified bacteriophage DNA contained both N6-methyladenine and N4-methylcytosine. The results presented here demonstrate that E125 is a new member of the supergroup of Siphoviridae that may be useful as a diagnostic tool for B. mallei.The disease glanders is caused by Burkholderia mallei, a host-adapted pathogen that does not persist in nature outside of its horse host (32). Glanders is a zoonosis, and humans whose occupations put them into close contact with infected animals can contract the disease. There have been no naturally occurring cases of glanders in North America in the last 60 years, but laboratory workers are still at risk of infection with B. mallei via cutaneous (68) and inhalational (31) routes. Human glanders has been described as a painful and loathsome disease from which few recover without antibiotic intervention (33, 51). There is little known about the virulence factors of this organism, but a recent report indicates that the capsular polysaccharide is essential for virulence in hamsters and mice (24).Burkholderia pseudomallei is the etiologic agent of the glanders-like disease melioidosis (21). As the names suggest, B. mallei and B. pseudomallei are closely related species (19,56,59,69). These ␤-Proteobacteria can now be directly compared at the genomic level because the B. pseudomallei K96243 genomic sequence is available at the Sanger Institute website (http://www.sanger.ac.uk/) and the B. mallei ATCC 23344 genomic sequence is available at the TIGR (The Institute for Genomic Research) website (http://www.tigr.org/). Preliminary BLAST (4) comparisons indicate that the genes conserved between these species are ϳ99% identical at the ...

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“…Mesmo havendo uma ótima concordância do ELISA-i quando comparado com o teste de ϐixação de complemento (FC) que possui sensibilidade em torno dos 90% a 95%, este último tem o risco de apresentar reações falso-negativas ocasionalmente observadas no soro de alguns animais (jovens, gestantes ou idosos), além de reações falso-positivas que podem ocorrer em aproximadamente 1% dos soros testados ao se utilizar antígeno de células inteiras, o que o leva a ser superado pelos resultados obtidos no ELISA-i (Cravitz & Miller 1950, Verma et al 1990. O animal gestante que apresentou resultado positivo no ELISA e negativo na Fixação de Complemento, provavelmente deve-se à limitação desse úlƟ mo teste, que pode apresentar resultados falsos-negativos, especialmente em soros de burros, mulas e animais gestantes (Gregory & Waag, 2008).…”

Section: Resultsunclassified

“…Embora a FC tenha uma sensibilidade de aproximadamente 90% a 95%, as reações falso-negativas são ocasionalmente observadas no soro de animais jovens, gestantes e idosos e reações falso-positivas também ocorrem em aproximadamente 1% dos soros testados onde atribui-se ao uso de antígeno composto por células inteiras (Cravitz & Miller 1950, OIE 2008a. Além disso, existem entraves quanto a padronização insuϐiciente do antígeno, o que pode comprometer a qualidade da reação, resultando em limitações no que diz respeito à especiϐicidade e sensibilidade da técnica.…”

Section: Introductionunclassified

Desenvolvimento e avaliação de um teste ELISA indireto para o diagnóstico sorológico do mormo em equídeos

et al. 2012

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Glanders is an infectious-contagious disease of acute or chronic character which principally affects horses, causing enormous losses in the productive chain of this animal. To control the disease, the Ministry of Agriculture, Husbandry and Supply instituted mandatory sanitation measures in the entire national territory which include an ofϐicial diagnosis through the complement ϐixation (CF) test, maleinization and sacriϐice of the animals that are positive. Nowadays the kits used for the diagnosis of the disease are imported, making their routine application difϐicult and more expensive. The objective of this study was to standardize an indirect ELISA test, using the proteic extract of Burkholderia mallei isolated from a carrier horse in the state of Pernambuco. The samples were cultivated in 10% blood agar and incubated for 48h at 37°C; later, one of the isolated colonies was characterized phenotypically and genotypically and immediately cultivated in brain heart infusion (BHI) for enrichment; then it was peaked (repicada) for the Dor-set Henley medium which was incubated at 37ºC under 60rpm for eight weeks. To standardize the test the Protein G Peroxidase Sigma Conjugate was used in the dilution of 1:90.000, with serums diluted in 1:100 and the antigen in 1:400. Sixty serums were used as negative controls, tested before the CF to determine the cutting point which was 0.042nm. After establishing the standardization, 300 samples were tested, of which 99% (297) were in agreement with the results obtained in the CF. At the end, of assay presented 100% sensibility and 98.2% speciϐicity, with predictive (preditivo) positive and negative values of 97.7% and 100% respectively. The Kappa concordance test was 0.98 and the intra and interplac repeatability were 8.8% and 10.3% respectively. From the results obtained, it is possible to afϐirm that the indirect ELISA test can be used as an efϐicient diagnosis tool. However, more essays must be carried out to consolidate the reliability of this test.

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Immunologic Studies with Malleomyces Mallei and Malleomyces Pseudomallei. I. Serological Relationships between M. Mallei and M. Pseudomallei

Cited by 32 publications

References 0 publications

A Comparative Study of Pseudomonas pseudomallei and Bacillus mallei

A Comparative Study of Pseudomonas pseudomallei and Bacillus mallei

Burkholderia thailandensis E125 Harbors a Temperate Bacteriophage Specific for Burkholderia mallei

Desenvolvimento e avaliação de um teste ELISA indireto para o diagnóstico sorológico do mormo em equídeos

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