Immunological analyses of the chemotactic receptor of Dictyosteleum discoideum. Identification of cDNA clones.

Klein, Claudette; Sadeghi, Homayoun; Simons, S

doi:10.1016/s0021-9258(18)66852-8

Cited by 7 publications

References 23 publications

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Characterization of an unusual cAMP receptor and its related polypeptides in Dictyostelium discoideum

et al. 1988

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Several lines of evidence indicate that cAMP modulates developmental gene activity via cell-surface receptors. We describe here a novel cAMP receptor, CABP1, whose properties are consistent with the idea that this protein is involved in gene regulation. Firstly, immunological techniques using anti-CABP1 antibodies as probes showed that this cAMP receptor can be detected on the surface of developing cells. Secondly, there is a steady migration of CABP1 to the nucleus during development. Thirdly, some genetic variants exhibiting an altered pattern of development are found to possess modified CABP1. We also showed that CABP1 co-purifies with at least seven other polypeptides which share common epitopes with CABP1. Interestingly, four of the CABP1-related polypeptides can be detected on the cell surface as well as in the nucleus.

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Characterization of an unusual cAMP receptor and its related polypeptides in Dictyostelium discoideum

et al. 1988

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Biosynthesis of 117 antigen: A cell cohesion molecule in Dictyostelium discoideum

1988

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117 antigen is involved in the process of intercellular cohesion in Dictyostelium discoideum [Brodie et al., 1983]. The antigen, a 69- and 72-kDa doublet, was found to arise from a 60- and 62-kDa precursor. The mature antigen contains N-linked oligosaccharides that are sulfated and fucosylated [Sadeghi et al., 1987]. These oligosaccharide chains are resistant to endoglycosidase H digestion. 117 antigen also contains a post-translationally added carbohydrate-containing modification(s). Unlike the N-linked oligosaccharide, this carbohydrate moiety is sensitive to periodate oxidation. 117 antigen is developmentally regulated, and the changes in rate of 117 antigen synthesis reflect changes in the cellular levels of its mRNA. 117 mRNA accumulates in starving cells and reaches its maximum when cells become aggregation competent. The mRNA levels then decline, and by the time the slug structure is formed, no 117 mRNA is present. 117 mRNA reaccumulates for a brief period during early culmination and then returns to an undetectable level.

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Chemokinesis

Houten¹,

Preston²

1998

Paramecium

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Immunological analyses of the chemotactic receptor of Dictyosteleum discoideum. Identification of cDNA clones.

Cited by 7 publications

References 23 publications

Characterization of an unusual cAMP receptor and its related polypeptides in Dictyostelium discoideum

Characterization of an unusual cAMP receptor and its related polypeptides in Dictyostelium discoideum

Biosynthesis of 117 antigen: A cell cohesion molecule in Dictyostelium discoideum

Chemokinesis

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