Immunological and allergenic responses induced by latex fractions of Calotropis procera (Ait.) R.Br.

Ramos, Márcio V.; Aguiar, Valéria C.; Melo, Vânia Maria Maciel; Mesquita, Rosilene Oliveira; Silvestre, Priscila P.; Oliveira, Jefferson Soares de; Oliveira, Raquel Sombra Basílio de; Macedo, Nívea Maria R.; Alencar, Nylane Maria Nunes de

doi:10.1016/j.jep.2006.10.034

Cited by 32 publications

(9 citation statements)

References 22 publications

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“…No death or toxic effects were observed. In another work, the same protein fraction of C. procera did not induce antibodies production when animals received latex proteins by oral route and therefore, did not develop allergy (Ramos et al, 2007). Accordingly, these results suggest that a possible toxic or allergenic reaction of cheeses produced with latex proteins of C. procera should be insignificant or null.…”

Section: Cheese Manufacture and Partial Characterizationmentioning

confidence: 67%

Insights into milk-clotting activity of latex peptidases from Calotropis procera and Cryptostegia grandiflora

Freitas

Leite

Oliveira

et al. 2016

Food Research International

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Latex fractions from Calotropis procera, Cryptostegia grandiflora, Plumeria rubra, and Himatanthus drasticus were assayed in order to prospect for new plant peptidases with milk-clotting activities, for use as rennet alternatives. Only C. procera and C. grandiflora latex fractions exhibited proteolytic and milk-clotting activities, which were not affected by high concentrations of NaCl and CaCl. However, pre-incubation of both samples at 75°C for 10min eliminated completely their activities. Both proteolytic fractions were able to hydrolyze k-casein and to produce peptides of 16kDa, a similar SDS-PAGE profile to commercial chymosin. RP-HPLC and mass spectrometry analyses of the k-casein peptides showed that the peptidases from C. procera or C. grandiflora hydrolyzed k-casein similar to commercial chymosin. The cheeses made with both latex peptidases exhibited yields, dry masses, and soluble proteins similar to cheeses prepared with commercial chymosin. In conclusion, C. procera and C. grandiflora latex peptidases with the ability to coagulate milk can be used as alternatives to commercial animal chymosin in the cheese manufacturing process.

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Section: Cheese Manufacture and Partial Characterizationmentioning

confidence: 67%

Insights into milk-clotting activity of latex peptidases from Calotropis procera and Cryptostegia grandiflora

Freitas

Leite

Oliveira

et al. 2016

Food Research International

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“…It seems that the water-soluble fraction of C. procera latex elicits mainly proinflammatory responses, whereas the water-insoluble fraction elicits anti-inflammatory responses. Findings suggesting proinflammatory action of the water-soluble fraction include edema after subcutaneous injection [5,10], arthritis induced by intraarticular injection [25], neutrophil migration elicited by intraperitoneal injection [2], and allergic responses induced by subcutaneous injection [26]. On the other hand, antiinflammatory actions of the water-insoluble fraction are suggested by findings that oral administration of methanol extract of C. procera latex inhibits inflammation in Freund's complete adjuvant-induced monoarthritis [27], and inhibits edema induced by carrageenin, histamine, serotonin, prostaglandinE2 or bradykinin [28].…”

Section: Discussionmentioning

confidence: 99%

Extract from Calotropis procera latex activates murine macrophages

et al. 2009

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Calotropis procera latex has long been used in traditional medicines. Extracts from C. procera latex have been reported to have various pharmacological actions, including protection from myocardial infarction, hepatoprotective action, antitumor activity, antinociceptive, and pro- and anti-inflammatory actions. To evaluate the immunomodulatory functions of the water-soluble C. procera extract (CPE), we investigated its ability to activate macrophages-effector cells in inflammatory and immune responses. Intraperitoneal injection of CPE in mice (2 mg/mouse) induced migration of macrophages to the intraperitoneal cavity, confirming the proinflammatory effects of water-soluble CPE. The direct effects of CPE on macrophages were then assessed by measuring the production of nitric oxide (NO) as an indicator for macrophage activation. Addition of CPE (1-10 microg/ml) to the culture medium of the murine monocyte/macrophage cell line RAW264.7 caused an increase in NO production in a time- and dose-dependent manner. CPE-elicited NO production was blocked by application of an inhibitor of inducible nitric oxide synthase (iNOS). Expression of iNOS mRNA was induced by treatment of cultured macrophages with CPE. Injection of CPE in mice also resulted in an increase in plasma NO level. The results suggest that CPE activates macrophages and facilitates NO production via up-regulation of iNOS gene expression.

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“…All parts of this plant have the ability to produce large quantities of latex when cut or broken [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

“…All parts of this plant have the ability to produce large quantities of latex when cut or broken [7][8][9].The plant latex could be separated after centrifugation into three layers: top layer containing Natural Rubber; middle layer of serum, and the bottom layer of lipids [9,10]. The chemical composition of latex is very complex, it contains (wt/wt) natural rubber poly (cis-1, 4-isoprene) 25 to 35%, which consider the main constituent of Natural rubber latex, and it is consisting of isoprene units C 5 H 8 in the cisconfiguration, a highly unsaturated hydrocarbon, with an average molecular weight about 10 6 Da [11][12][13].…”

mentioning

confidence: 99%

Biodegradation of Natural Rubber Latex of Calotropis procera by Two Endophytic Fungal Species

Mohamed¹,

Ismail²,

Abdel‐Mageed³

et al. 2017

J Bioremediat Biodegrad

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IntroductionCalotropis is a small genus belonging to family Asclepiadaceae, Calotropis gigantea and C. procera are the two most common species in this genus used in traditional medicine, and only these two species are usually reported in literature [1], and are distributed in tropical and subtropical Africa, Asia, and America [2][3][4][5].Calotropis procera (Ait) WT Aiton is a small wild-growing tropical plant, known by different names like sodom apple, usher, dead sea apple, swallow wort, and milk weed [6,7]. All parts of this plant have the ability to produce large quantities of latex when cut or broken [7][8][9].The plant latex could be separated after centrifugation into three layers: top layer containing Natural Rubber; middle layer of serum, and the bottom layer of lipids [9,10]. The chemical composition of latex is very complex, it contains (wt/wt) natural rubber poly (cis-1, 4-isoprene) 25 to 35%, which consider the main constituent of Natural rubber latex, and it is consisting of isoprene units C 5 H 8 in the cisconfiguration, a highly unsaturated hydrocarbon, with an average molecular weight about 10 6 Da [11][12][13].Natural Rubber is a basic material for manufacturing tires, latex gloves, condoms, seals, balloons, balls for sports and many other things [14]. Degradation potential of the microorganism is dependent upon colonization on natural rubber and accompanied by a loss in the weight of the rubber hydrocarbon and a decline in the relative viscosity of the polymer solution [13].Many other reports have been published on the biodegradation of natural rubber hydrocarbon as a sole carbon source by bacteria [15][16][17][18][19][20][21][22], fungi [13,23,24]. Several microbes were used to biodegrade Calotropis Procera latex [25,26].The aim of this work is to assess the ability of mesophilic endophytic fungi isolated from Calotropis procera leaves and latex on sugar-free Czapek agar and to test their ability to metabolize and degrade rubber latex of C. procera as a sole carbon source. AbstractIn this study eight species related to Aspergillus (3 species), Fusarium (1), Penicillium (1), Emericella (1), Nigrospora (1) and trichoderma (1) were isolated from leaves and latex of Calotropis procera. Only P. chrysogenium and A. niger were able to grow on natural rubber but other species were not. The degrading ability of (Penicillium chrysogenum and Aspergillus niger), isolated from latex of calotropis procera was assessed. The degradation of rubber latex was determined by measuring the increase in protein content of the fungus (mg/g dry wt), reduction in molecular weight (g/mol) and inherent viscosity (dl/g) of the latex. Moreover, the degradation was also confirmed by observing the growth of these species strain using scanning electron microscopy.

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Immunological and allergenic responses induced by latex fractions of Calotropis procera (Ait.) R.Br.

Cited by 32 publications

References 22 publications

Insights into milk-clotting activity of latex peptidases from Calotropis procera and Cryptostegia grandiflora

Insights into milk-clotting activity of latex peptidases from Calotropis procera and Cryptostegia grandiflora

Extract from Calotropis procera latex activates murine macrophages

Biodegradation of Natural Rubber Latex of Calotropis procera by Two Endophytic Fungal Species

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