Immunological and biochemical responses in mice treated with mercuric chloride

Dieter, Michael P.; Luster, Michael I.; Boorman, Gary A.; Jameson, C. W.; Dean, Jack H.; Cox, James H.

doi:10.1016/0041-008x(83)90006-6

Cited by 87 publications

(27 citation statements)

References 38 publications

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“…Several chemicals in the mixture of groundwater contaminants are cytotoxic, including arochlor, benzene, phenol, and heavy metals (Faith et al 1979;Levis and Majone 1979;Tunek et al 1981;Wierda et al 1981;Beran et al 1983;Bolcsak and Nerland 1983;Dieter et al 1983Dieter et al , 1988Keller and Snyder 1986;Hong et al 1989). The myelotoxic effects observed are probably a composite response by the various chemicals and/or their metabolites rather than the effect of one individual component.…”

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confidence: 98%

Enhancement of myelotoxicity induced by repeated irradiation in mice exposed to a mixture of groundwater contaminants

1993

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As part of a program on the toxicology of chemical mixtures at the National Institute of Environmental Health Sciences/National Toxicology Program (NIEHS/NTP), hematopoietic functions were studied in female B6C3F1 mice treated with 0, 1%, and 5% of a chemical mixture stock of 25 groundwater contaminants in drinking water for 31.5 weeks. The toxicologic interaction between continuous exposure to groundwater contaminants and stress induced by multiple irradiation on hematopoiesis was investigated. For those mice receiving both the chemical mixture and irradiation, the exposure to the former was continuous throughout the 31.5-week experimental period, whereas whole body irradiations (4 times at 200 rads/each) were carried out at 7-week intervals with the first one at 3.5 weeks. Myelotoxicity assessment was made by determining the number of granulocyte-macrophage progenitor cells (CFU-GM) 1 week after each irradiation and also at 6 weeks following irradiation as a measure of recovery from stress. Non-irradiated mice treated with 5% chemical mixture solution showed suppression of CFU-GM after 15.5 weeks and became progressively more affected (only 70% of controls by 31.5 weeks of treatment). The population of CFU-GM in mice treated with 5% chemical mixture for 4.5 weeks plus irradiation (1 week after first irradiation) was only 22% of the non-irradiated vehicle control group. This combined (i.e., chemical mixture plus irradiation) suppression of CFU-GM intensified after repeated irradiation until the number of CFU-GM was only 10.7% following the fourth irradiation at 25.5 weeks. Thus, irradiation caused a significant reduction in CFU-GMs in all mice but the effects were more pronounced in mice treated with a chemical mixture.(ABSTRACT TRUNCATED AT 250 WORDS)

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confidence: 98%

Enhancement of myelotoxicity induced by repeated irradiation in mice exposed to a mixture of groundwater contaminants

1993

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“…Similarly, Sauvé et al (2002b) showed that Cd, Cu, Ni, and Zn caused significant immunosuppressive effects with concentrations inducing 50 % inhibition ranging from 10 −5 to 10 −4 M in Eisenia fetida, Lumbricus terrestris, Aporrectodea turgida, and Tubifex tubifex. Finally, an increase of phagocytic activity after exposure to contaminants was previously demonstrated in bivalves, rodents, reptiles, birds, fishes, bovine, whales and primates Dieter et al 1983;Voccia et al 1994;De Guise et al 1997). Among hypothetical mechanisms, Brousseau et al (2000) proposed that cadmium may stimulate directly some metabolic processes involved in phagocytosis of Mya arenaria haemocytes.…”

Section: Discussionmentioning

confidence: 83%

Does a short-term exposure to cadmium chloride affects haemocyte parameters of the marine gastropod Haliotis tuberculata?

Ladhar-Chaabouni¹,

Machreki-Ajmi²,

Serpentini

et al. 2014

Environ Sci Pollut Res

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In this study, a model based on primary cultured haemocytes from the gastropod mollusc Haliotis tuberculata was established to investigate the effects of cadmium chloride in vitro. Cells were exposed for 24 h to CdCl2 concentrations of 0, 1 and 100 μg ml(-1). The effects of cadmium on haemocyte parameters were investigated using morphological, spectrophotometric and flow cytometry analysis. Results showed that cadmium has no significant effects on cell viability and phagocytotic activity under the tested conditions. However, haemocytes became more rounded after cadmium exposure, which could explain the significant decrease of cell area beginning at 1 μg ml(-1) of CdCl2.

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“…5 Cytotoxicity of Cd to haemocytes assessed by the neutral red assay (a) and XTT assay (b). Haemocytes were exposed to indicated Cd concentrations for 24 h. Data are means of 11 measurements (Olabarrieta et al 2001) as rodents, reptiles, birds, fishes, bovines, whales and primates exposed to mercury and cadmium (Bernier et al 1995;De Guise et al 1997;Dieter et al 1983;Voccia et al 1994). For bivalves, it was proposed that the stimulation of phagocytosis by divalent cations might be the result of decreased surface charge through binding on negatively charged groups, leading to an enhanced binding and subsequent phagocytosis on nonpolar particles (Cheng and Sullivan 1984).…”

Section: Phagocytosis Activitymentioning

confidence: 99%

The cell cultures and the use of haemocytes from marine molluscs for ecotoxicology assessment

Ladhar-Chaabouni

Hamza-Chaffai

2015

Cytotechnology

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Among aquatic organisms suitable for biological monitoring, molluscs occupy a prominent place due to their wide geographic distribution, their abundance and accessibility in the field as well as in aquaculture. Molluscs reflect the degree of environmental contamination and are the most useful bioindicator tools. The study of modulation of immune system or immunomodulation in marine molluscs has become one of the privileged ways for evaluating the physiological effects of environmental factors. Physiological responses of molluscs to environmental stresses could be mediated by haemocytes. These cells are continually exposed to the external environment due to the open circulatory system of molluscs and are affected by pollutants. In fact, several studies showed the effects of different environmental contaminants on haemocyte functions (viability, phagocytosis, ROS production) as well as on proteins involved in cytoskeletal structure maintenance using the in vitro approaches. In ecotoxicology, in vitro approach is an alternative to animal testing due to the reduced use of experimental animals, low cost and rapid performance. Although several studies showed the importance of using in vitro cell models to determine the effects of different environmental contaminants on haemocyte parameters in marine molluscs, a few reviews highlight these effects. The main purpose of this paper is to summarize the recent data on the effect of some xenobiotics on haemocyte parameters in some mollusc species and then suggest future research prospects.

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Immunological and biochemical responses in mice treated with mercuric chloride

Cited by 87 publications

References 38 publications

Enhancement of myelotoxicity induced by repeated irradiation in mice exposed to a mixture of groundwater contaminants

Enhancement of myelotoxicity induced by repeated irradiation in mice exposed to a mixture of groundwater contaminants

Does a short-term exposure to cadmium chloride affects haemocyte parameters of the marine gastropod Haliotis tuberculata?

The cell cultures and the use of haemocytes from marine molluscs for ecotoxicology assessment

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