Immunological and Chemical Identification of Intracellular Forms of Adenovirus Type 2 Terminal Protein

Green, Maurice; Symington, Janey; Brackmann, Karl H.; Cartas, Maria; Thornton, Helen; Young, Leann

doi:10.1128/jvi.40.2.541-550.1981

Cited by 15 publications

(2 citation statements)

References 33 publications

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“…In this manuscript, the contribution of the exon at genome coordinate 39 to the production of functional pTP from cloned DNA in plasmid vectors has been determined. Although the addition of this upstream exon had no apparent effect on the molecular mass or the amounts of an 80-kDa polypeptide that could be immunoprecipitated with antibody against TP (14), there was a dramatic effect on the activity of the expressed pTP: only the construct containing the HindIII J fragment (genome coordinate 39) was active. Thus, the 3.…”

Section: Discussionmentioning

confidence: 96%

Adenovirus preterminal protein synthesized in COS cells from cloned DNA is active in DNA replication in vitro

Pettit

Horwitz

Engler

1988

J Virol

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Replication of the DNA genome of human adenovirus serotype 2 requires three virus-encoded proteins. Two of these proteins, the preterminal protein (pTP) and the adenovirus DNA polymerase, are transcribed from a single promoter at early times after virus infection. The mRNAs for these proteins share several exons, including one encoded near adenovirus genome coordinate 39. By using plasmids containing DNA fragments postulated to encode the various exons of pTP mRNA, the contributions of each exon to the synthesis of an active pTP have been measured. Only plasmids that contain both the open reading frame for pTP (genome coordinates 29.4 to 23.9) and the HindIII J fragment that contains the exon at genome coordinate 39 can express functional pTP.

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Section: Discussionmentioning

confidence: 96%

Adenovirus preterminal protein synthesized in COS cells from cloned DNA is active in DNA replication in vitro

Pettit

Horwitz

Engler

1988

J Virol

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“…OKtht information. The immunoprecipitate was analyzed by a of both sodium dodecyl sulfate-polyacrylamide gel electrocells; this phoresis (SDS-PAGE) and autoradiography (14). dried gels by autoradiography, were digested with trypsin and analyzed by two-dimensional electrophoresis (pH 1.7)-thin-layer chromatography (16).…”

Section: Methodsmentioning

confidence: 99%

Identification and purification of a protein encoded by the human adenovirus type 2 transforming region

Green¹,

Brackmann²,

Cartas³

et al. 1982

J Virol

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The human adenovirus type 2 (Ad2) transforming genes are located in early regions Ela (map position 1.3 to 4.5) and Elb (map position 4.6 to 11.2). We have identified and purified to near homogeneity a major 20,000-molecular-weight (20K) protein and have shown that it is coded by Elb. Using an Ad2-transformed cell antiserum which contained antibody to Elb-coded proteins, we immunoprecipitated 53K and 19K proteins from the nucleoplasm and 53K, 19K, and 20K proteins from the cytoplasmic S-100 fraction of Ad2 productively infected and Ad2-transformed cells. The 19K protein was present in both the nucleoplasm and the cytoplasm, whereas the 20K protein was found only in the cytoplasm. The 53K and 19K proteins are known Ad2 Elb-coded proteins. The 20K protein was purified to near homogeneity in 20 to 50% yields by sequential DEAE-Sephacel chromatography and reverse-phase high-performance liquid chromatography. Purified 20K protein shares most of its methionine-labeled tryptic peptides with Elb-53K, as shown by reverse-phase high-performance liquid chromatography, and therefore is closely related to the 53K protein. The 19K protein does not appear to share tryptic peptides with either 20K or 53K protein. To provide more direct evidence that 20K protein is virus-coded, we translated Elb-specific mRNA in vitro. Both immunoprecipitation analysis and high-performance liquid chromatography purification of the translated product identified a 20K protein that has the same tryptic peptides as the 20K protein isolated from infected and from transformed cells. These findings suggest that the Ad2 20K protein is a primary translation product of an Ad2 Elb mRNA.

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The Origin of Adenovirus DNA Replication

Tamanoi

Stillman

1983

Current Topics in Microbiology and Immunology

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Immunological and Chemical Identification of Intracellular Forms of Adenovirus Type 2 Terminal Protein

Cited by 15 publications

References 33 publications

Adenovirus preterminal protein synthesized in COS cells from cloned DNA is active in DNA replication in vitro

Adenovirus preterminal protein synthesized in COS cells from cloned DNA is active in DNA replication in vitro

Identification and purification of a protein encoded by the human adenovirus type 2 transforming region

The Origin of Adenovirus DNA Replication

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