Immunological and Clinical Specificity of the Immunochemical Determination of Prostatic Acid Phosphatase

Shaw, Leslie M.; Na, Yang; Neat, Marilyn; Croop, William

doi:10.1111/j.1749-6632.1982.tb40305.x

Cited by 22 publications

(2 citation statements)

References 23 publications

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“…Immunoreactive PAP in spot (untimed) urine samples has re cently been reported by Shaw cl al. [10], thus confirming our results. The origin and characteristics of this PAP immunoreactivity is not known.…”

Section: Discussionsupporting

confidence: 83%

Analysis of Prostatic Acid Phosphatase in Urine Voided before and after Massage of the Prostate in Infertile Men

1984

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Prostatic acid phosphatase (PAP) – a specific marker of prostatic secretion – has been analyzed by radioimmunoassay in samples of urine voided before and after massage of the prostate. In all patients there was an increase in urine PAP concentration after massage irrespective of the occurrence of expressed prostatic secretion. The mean PAP values increased from 4.9 to 45.8 µg/l. No significant change in urine creatinine concentration was observed. No lactoferrin was detected in the urine which should indicate that no secretion from the seminal vesicles was expressed into the urethra during massage of the prostate. Analysis of PAP in urine after massage of the prostate could increase the value of a urine sediment and microbial analysis in the investigation of male infertility.

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Section: Discussionsupporting

confidence: 83%

Analysis of Prostatic Acid Phosphatase in Urine Voided before and after Massage of the Prostate in Infertile Men

1984

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“…The failure to detect other types of P-Tyr-protein phosphatase activity may simply reflect the fact that the acid phosphatase is present in large amounts in prostate (0.86 mg/g of wet tissue) [48]. Due to the limited availability of [32P]P-Tyr-proteins, the substrate specificity of the prostatic enzyme has been studied using nanomolar concentrations of substrates.…”

Section: Discussionmentioning

confidence: 99%

A phosphotyrosyl‐protein phosphatase activity associated with acid phosphatase from human prostate gland

Chernoff

Chen

et al. 1984

European Journal of Biochemistry

108

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Using [32P]P-Tyr-IgG and [32P]P-Tyr-casein phosphorylated by pp6O"-"c as substrates, studies on the phosphotyrosyl-protein phosphatase activity in human prostate gland indicate that it is associated with prostatic acid phosphatase. Evidence to support this conclusion include the following : (a) these two enzymatic activities co-purify to apparent homogeneity; (b) they co-migrated on polyacrylamide gel electrophoresis, ion-exchange and gel filtration chromatographies; (c) the exhibit identical thermostability; and (d) the phosphotyrosyl-protein phosphatase activity is sensitive to inhibition by p-nitrophenyl phosphate and by several classical inhibitors of prostatic acid phosphatase including I.(+)-tartrate, molybdate, vanadate and NaF. The purified enzyme exhibits high specificity towards phosphotyrosyl-proteins with little activity towards several phosphoseryl-proteins and phosphothreonyl-proteins examined. The present findings indicate that prostatic acid phosphatase may function in vivo as a phosphotyrosyl-protein phosphatase.Several transforming proteins, such as-pp60" coded by onc genes of viral or cellular origin have been shown to possess phosphotyrosyl-protein (P-Tyr-protein) kinase activity [ 1 -91. P-Tyr-protein kinase activity has also been shown to be associated with receptors for growth-promoting peptides, such as epidermal growth factor (EGF) [lo, 1 I], platelet-derived growth factor (PDGF) [12, 131 and insulin [14, 151. These findings have led to the hypothesis that phosphorylation at Tyr residues in proteins plays an important role in cellular transformation and in regulation of cellular growth [16,17].Several studies concerning P-Tyr-protein phosphatase activity in animal tissues have appeared recently. Alkaline phosphatases of animal and bacterial origin [18,19] and an acid phosphatase activity in the plasma membrane of human astrocytoma [20] have been reported to exhibit high specificity towards P-Tyr-histones. On the other hand, various P-Tyrprotein phosphatases lacking activity towards low-molecularweight non-protein phosphoesters, such asp-nitrophenyl phosphate (pNpP), have also been described [21-241. In this communication we examine P-Tyr-protein phosphatase activity in human prostate gland. The results indicate that the major active species of P-Tyr-protein phosphatase activity in this tissue is associated with acid phosphatasese, which has served as a useful biological marker of metastatic prostatic carcinoma for the last four decades [25 -281.

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Immunoelectron microscopic demonstration of prostatic acid phosphatase in human hyperplastic prostate

Gang

Lin

et al. 1985

The Prostate

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Immunoelectron microscopic studies were done on prostatic tissues obtained from patients with benign hyperplasia. Rabbit IgG-peroxidase conjugate against purified human prostatic acid phosphatase band 2 (HPAP-2) was used for studies. Under the light microscope, the columnar secretory epithelia of prostatic glands showed different intensity and distribution of immunostaining whereas the basal cells were unstained. Under the electron microscope, the secretory epithelial cells often showed electron-dense reaction product in the Golgi apparatus and secretory vesicles and vacuoles, and only sparingly in the cisternae of nuclear envelope and rough ER. Sometimes, fusion of secretory vacuolar membrane and plasma membrane and discharge of the vacuolar contents into the extracellular space were noted. The surfaces of microvilli at the apical portion of the columnar epithelia and the lumen of the glandular acini always showed reaction product. These findings suggest that HPAP-2 may be synthesized in the rough ER and transported to the Golgi apparatus where it is concentrated and transferred to the secretory vesicles and vacuoles. HPAP-2 is finally discharged into the extracellular spaces through exocytosis, a secretory mechanism similar to that of other secretory proteins.

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Immunological and Clinical Specificity of the Immunochemical Determination of Prostatic Acid Phosphatase

Cited by 22 publications

References 23 publications

Analysis of Prostatic Acid Phosphatase in Urine Voided before and after Massage of the Prostate in Infertile Men

Analysis of Prostatic Acid Phosphatase in Urine Voided before and after Massage of the Prostate in Infertile Men

A phosphotyrosyl‐protein phosphatase activity associated with acid phosphatase from human prostate gland

Immunoelectron microscopic demonstration of prostatic acid phosphatase in human hyperplastic prostate

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